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Information on EC 6.5.1.4 - RNA 3'-terminal-phosphate cyclase (ATP)

for references in articles please use BRENDA:EC6.5.1.4
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IUBMB Comments
The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in an ATP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a histidine residue [5,6]. The adenylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]-3'-(5'-diphosphoadenosine). Finally, the enzyme catalyses an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the adenylate. The enzyme also has a polynucleotide 5' adenylylation activity . cf. EC 6.5.1.5, RNA 3'-terminal-phosphate cyclase (GTP).
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Word Map
  • 6.5.1.4
  • phosphodiester
  • adenylate
  • ligases
  • nucleotidylation
  • cyclases
  • adenylylation
  • eucarya
  • pre-rrna
  • polynucleotide
  • 3',5'-phosphodiester
  • 2'-phosphate
  • oligoribonucleotides
  • alpha-32patp
  • cyclase-like
  • ribozyme
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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[RNA]-3'-(3'-phospho-ribonucleoside)
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[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
Synonyms
rcl1p, rna 3'-terminal phosphate cyclase, rna 3'-phosphate cyclase, rna cyclase, st-rtc, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [RNA 3'-phosphate cyclase]-L-histidine = [RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
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(1a)
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ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
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[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside) = [RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
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(1b)
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[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine) = [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
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(1c)
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