Information on EC 6.3.2.52 - jasmonoyl-L-amino acid synthetase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.3.2.52
-
RECOMMENDED NAME
GeneOntology No.
jasmonoyl-L-amino acid synthetase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + jasmonate + an L-amino acid = AMP + diphosphate + a jasmonoyl-L-amino acid
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
jasmonate:L-amino acid ligase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
enzyme silencing impairs jasmonic acid-isoleucine-mediated defenses against Manduca sexta
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (+)-jasmonate + L-Ile
AMP + diphosphate + jasmonoyl-L-Ile
show the reaction diagram
-
very low activity
-
-
?
ATP + (+/-)-jasmonate + L-Ile
AMP + diphosphate + jasmonoyl-L-Ile
show the reaction diagram
-
-
-
-
?
ATP + (-)-jasmonate + L-Gln
AMP + diphosphate + jasmonoyl-L-Gln
show the reaction diagram
-
-
-
-
?
ATP + (-)-jasmonate + L-Ile
AMP + diphosphate + jasmonoyl-L-Ile
show the reaction diagram
ATP + (-)-jasmonate + L-Leu
AMP + diphosphate + jasmonoyl-L-Leu
show the reaction diagram
ATP + (-)-jasmonate + L-Phe
AMP + diphosphate + jasmonoyl-L-Phe
show the reaction diagram
-
-
-
-
?
ATP + (-)-jasmonate + L-Val
AMP + diphosphate + jasmonoyl-L-Val
show the reaction diagram
ATP + 12-oxophytodienoate + L-Ile
AMP + diphosphate + 12-oxophytodienoyl-L-Ile
show the reaction diagram
-
low activity
-
-
?
ATP + 13-hydroperoxylinolenoate + L-Ile
AMP + diphosphate + 13-hydroperoxylinolenoyl-L-Ile
show the reaction diagram
-
low activity
-
-
?
ATP + 3-oxocyclopentane octanoate + L-Ile
AMP + diphosphate + 3-oxocyclopentane octanoyl-L-Ile
show the reaction diagram
-
low activity
-
-
?
ATP + 9,10-dihydro-jasmonate + L-Ile
?
show the reaction diagram
-
-
-
-
?
ATP + jasmonate + an L-amino acid
AMP + diphosphate + a jasmonoyl-L-amino acid
show the reaction diagram
ATP + jasmonate + L-Ile
AMP + diphosphate + jasmonoyl-L-Ile
show the reaction diagram
ATP + linolenate + L-Ile
AMP + diphosphate + linolenoyl-L-Ile
show the reaction diagram
-
low activity
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (-)-jasmonate + L-Ile
AMP + diphosphate + jasmonoyl-L-Ile
show the reaction diagram
-
L-Ile is the preferred amino acid
-
-
?
ATP + jasmonate + an L-amino acid
AMP + diphosphate + a jasmonoyl-L-amino acid
show the reaction diagram
ATP + jasmonate + L-Ile
AMP + diphosphate + jasmonoyl-L-Ile
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
(-)-jasmonate
-
at pH 8.6 and 25°C
-
0.03
L-Ile
-
at pH 8.6 and 25°C
1.93
L-Leu
-
at pH 8.6 and 25°C
1.84
L-Phe
-
at pH 8.6 and 25°C
2.49
L-Val
-
at pH 8.6 and 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
jarin-1
Arabidopsis thaliana;
-
pH and temperature not specified in the publication
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00024
-
using (+)-jasmonate as substrate, at pH 8.6 and 25°C
0.00756
-
using 9,10-dihydro-jasmonate as substrate, at pH 8.6 and 25°C
0.0105
-
using (+/-)-jasmonate as substrate, at pH 8.6 and 25°C
0.0298
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using (-)-jasmonate as substrate, at pH 8.6 and 25°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 95000, GST-tagged enzyme, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme transcripts are strongly elicitated by wounding and treatment with Manduca sexta oral secretions
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the enzyme transcript increases dramatically in wounded tissue, reaching a maximum after about 1 h
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the enzyme-dependent jasmonate signaling pathway is activated upon exposing Arabidopsis to (E)-2-hexenal or (Z)-3-hexenal
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