Information on EC 6.3.2.14 - enterobactin synthase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
6.3.2.14
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RECOMMENDED NAME
GeneOntology No.
enterobactin synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxamide formation
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-
-
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carboxylic acid amide formation
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of siderophore group nonribosomal peptides
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxybenzoate:L-serine ligase
This enzyme complex catalyses the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyses the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyses the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3-dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
CAS REGISTRY NUMBER
COMMENTARY hide
37318-63-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
chorismate is converted to 2,3-dihydroxybenzoate via the sequential catalytic activities of EntC, -B, and -A; chorismate is converted to 2,3-dihydroxybenzoate via the sequential catalytic activities of EntC, -B, and -A; chorismate is converted to 2,3-dihydroxybenzoate via the sequential catalytic activities of EntC, -B, and -A
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine
enterobactin + 6 AMP + 6 diphosphate
show the reaction diagram
ATP + 2,3-dihydroxybenzoate + L-Ser
Products of ATP breakdown + N-(2,3-dihydroxybenzoyl)-L-Ser
show the reaction diagram
ATP + 2,3-dihydroxybenzoate + L-serine
N-(2,3-dihydroxybenzoyl)-L-serine + AMP + diphosphate
show the reaction diagram
ATP + 2,3-dihydroxybenzoate + phosphopantetheinylated EntB
arylated EntB + AMP + diphosphate
show the reaction diagram
reaction of EntE
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-
?
ATP + 3-hydroxybenzoate + phosphopantetheinylated EntB
arylated EntB + AMP + diphosphate
show the reaction diagram
reaction of EntE
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-
?
ATP + 4-aminosalicylic acid + phosphopantetheinylated EntB
arylated EntB + AMP + diphosphate
show the reaction diagram
reaction of EntE
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-
?
ATP + arylated EntB + L-serine
enterobactin + AMP + diphosphate
show the reaction diagram
reaction of EntF
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-
?
ATP + salicylic acid + phosphopantetheinylated EntB
arylated EntB + AMP + diphosphate
show the reaction diagram
reaction of EntE
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine
enterobactin + 6 AMP + 6 diphosphate
show the reaction diagram
ATP + 2,3-dihydroxybenzoate + L-serine
N-(2,3-dihydroxybenzoyl)-L-serine + AMP + diphosphate
show the reaction diagram
ATP + 2,3-dihydroxybenzoate + phosphopantetheinylated EntB
arylated EntB + AMP + diphosphate
show the reaction diagram
P0ADI4, P10378, P11454
reaction of EntE
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?
ATP + arylated EntB + L-serine
enterobactin + AMP + diphosphate
show the reaction diagram
P0ADI4, P10378, P11454
reaction of EntF
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-O-[N-(2,3-dihydroxybenzoyl)sulfamoyl]adenosine
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5'-O-[N-(salicyl)sulfamoyl]adenosine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0025
2,3-Dihydroxybenzoate
pH 7.8, 25°C, recombinant EntE
0.07
3-hydroxybenzoate
pH 7.8, 25°C, recombinant EntE
3.1
4-aminosalicylic acid
pH 7.8, 25°C, recombinant EntE
0.43
ATP
pH 7.8, 25°C, recombinant EntE
260
L-Ser
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ATP-diphosphate exchange
0.0029
phosphopantetheinylated EntB
pH 7.8, 25°C, recombinant EntE
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0.07
salicylic acid
pH 7.8, 25°C, recombinant EntE
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.8
2,3-Dihydroxybenzoate
pH 7.8, 25°C, recombinant EntE
0.3
3-hydroxybenzoate
pH 7.8, 25°C, recombinant EntE
4.4
4-aminosalicylic acid
pH 7.8, 25°C, recombinant EntE
2.8
ATP
pH 7.8, 25°C, recombinant EntE
12.7
L-Ser
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L-Ser-dependent ATP-diphosphate exchange
2.8
phosphopantetheinylated EntB
pH 7.8, 25°C, recombinant EntE
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0.8
salicylic acid
pH 7.8, 25°C, recombinant EntE
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
880
2,3-Dihydroxybenzoate
pH 7.8, 25°C, recombinant EntE
4.6
3-hydroxybenzoate
pH 7.8, 25°C, recombinant EntE
15
4-aminosalicylic acid
pH 7.8, 25°C, recombinant EntE
390
ATP
pH 7.8, 25°C, recombinant EntE
980
phosphopantetheinylated EntB
pH 7.8, 25°C, recombinant EntE
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11
salicylic acid
pH 7.8, 25°C, recombinant EntE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Escherichia coli (strain K12);
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
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1 * 59000, deduced from nucleotide sequence
66000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 59000, deduced from nucleotide sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant EntE
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recombinant His-tagged EntE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene entE, expression in Escherichia coli strain BL21(DE3); gene entE, expression in Escherichia coli strain BL21(DE3); gene entE, expression of His-tagged EntE in Escherichia coli strain BL21(DE3)
overexpression of EntE in Escherichia coli
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