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The expected taxonomic range for this enzyme is: Thermoplasma acidophilum
Synonyms
minichromosome maintenance protein,
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minichromosome maintenance protein
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DNA 3'-5' helicase (ATP-hydrolysing)
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ATP + H2O
ADP + phosphate
the protein binds single-stranded DNA, has DNA-dependent ATPase activity and ATP-dependent 3'->5' helicase activity
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Mg2+
binding to ATP and to DNA is required for helicase activity
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additional information
the enzyme is more active in Tris and Bicine buffers than Hepes and phosphate buffers
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additional information
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the enzyme is more active in Tris and Bicine buffers than Hepes and phosphate buffers
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53 - 70
53°C: about 45% of maximal activity, 70°C: about % of maximal activity
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UniProt
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Q9HK10_THEAC
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
698
0
78899
TrEMBL
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Haugland, G.; Rollor, C.; Birkeland, N.; Kelman, Z.
Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum
Extremophiles
13
81-88
2009
Thermoplasma acidophilum (Q9HK10), Thermoplasma acidophilum
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