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IUBMB Comments This enzyme has been characterized from the bacterium Pseudomonas putida KT2440 and is involved in the degradation pathway of syringate and 3,4,5-trihydroxybenzoate. It catalyses the interconversion of two of the tautomers of 4-oxalomesaconate, a reaction that can also occur spontaneously.
The enzyme appears in viruses and cellular organisms
Synonyms oma tautomerase, more
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4-oxalomesaconic acid tautomerase
EC 5.3.3.16
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formerly
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4-oxalomesaconic acid tautomerase
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4-oxalomesaconic acid tautomerase
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OMA tautomerase
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pmdU
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(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate = (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate
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MetaCyc
gallate degradation I, gallate degradation II, methylgallate degradation, protocatechuate degradation I (meta-cleavage pathway), syringate degradation
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4-oxalomesaconate keto-enol-isomerase
This enzyme has been characterized from the bacterium Pseudomonas putida KT2440 and is involved in the degradation pathway of syringate and 3,4,5-trihydroxybenzoate. It catalyses the interconversion of two of the tautomers of 4-oxalomesaconate, a reaction that can also occur spontaneously.
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(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
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Substrates: - Products: -
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(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
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(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
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Substrates: - Products: -
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(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
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4-chloronitrobenzene
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4-chloronitrobenzene
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(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
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Substrates: - Products: -
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(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
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Substrates: - Products: -
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Highest Expressing Human Cell Lines
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malfunction
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disruption of the galD gene does not prevent growth of the Pseudomonas putida KTGALdgalD mutant strain in 5 mM gallic acid, but the mutant strain grows poorly at gallic acid concentrations lower than 2 mM
malfunction
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disruption of the galD gene does not prevent growth of the Pseudomonas putida KTGALdgalD mutant strain in 5 mM gallic acid, but the mutant strain grows poorly at gallic acid concentrations lower than 2 mM
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physiological function
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a functional galD gene is important for an efficient gallic acid degradation
physiological function
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a functional galD gene is important for an efficient gallic acid degradation
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37600
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calculated from amino acid sequence
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expressed in Escherichia coli BL21(DE3) cells
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Nogales, J.; Canales, A.; Jimenez-Barbero, J.; Serra, B.; Pingarron, J.M.; Garcia, J.L.; Diaz, E.
Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida
Mol. Microbiol.
79
359-374
2011
Pseudomonas putida, Pseudomonas putida KT 2240
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Ni, B.; Zhang, Y.; Chen, D.W.; Wang, B.J.; Liu, S.J.
Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5-cleavage pathway in bacteria
Appl. Microbiol. Biotechnol.
97
6031-6041
2013
Comamonas testosteroni, Comamonas testosteroni CNB-1
brenda
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