Reference on EC 5.1.3.14 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Spivak, C.T.; Roseman, S.
UDP-N-acetyl-D-glucosamine 2'-epimerase
Methods Enzymol.
9
612-615
1966
Rattus norvegicus
-
Salo, W.L.; Fletcher, H.G.
Studies on the mechanism of action of uridine diphosphate N-acetylglucosamine 2-epimerase
Biochemistry
9
882-885
1970
Rattus norvegicus
Sommar, K.M.; Ellis, D.B.
Uridine diphosphate N-acetyl-D-glucosamine 2-epimerase from rat liver. I. Catalytic and regulatory properties
Biochim. Biophys. Acta
268
581-589
1972
Rattus norvegicus
Sommar, K.M.; Ellis, D.B.
Uridine diphosphate N-acetyl-D-glucosamine 2-epimerase from rat liver. II. Studies on the mechanism of action
Biochim. Biophys. Acta
268
590-595
1972
Rattus norvegicus
Kikuchi, K.; Tsuiki, S.
Purification and properties of UDP-N-acetylglucosamine 2'-epimerase from rat liver
Biochim. Biophys. Acta
327
193-206
1973
Rattus norvegicus
Kawamura, T.; Ichihara, N.; Ishimoto, N.; Ito, E.
Biosynthesis of uridine diphosphate N-acetyl-D-mannosaminuronic acid from uridine diphosphate N-acetyl-D-glucosamine in Escherichia coli: separation of enzymes responsible for epimerization and dehydrogenation
Biochem. Biophys. Res. Commun.
66
1506-1512
1975
Escherichia coli
Salo, W.L.
The incorporation of tritium from tritium-enriched water into UDP-N-acetylglucosamine and UDP-N-acetyl-mannosamine catalyzed by UDP-N-acetylglucosamine 2-epimerase from Escherichia coli
Biochim. Biophys. Acta
452
625-628
1976
Escherichia coli
Kawamura, T.; Kimura, M.; Yamamori, S.; Ito, E.
Enzymatic formation of uridine diphosphate N-acetyl-D-mannosamine
J. Biol. Chem.
253
3595-3601
1978
Bacillus cereus, Priestia megaterium, Bacillus subtilis, Escherichia coli, Micrococcus luteus, Staphylococcus aureus
Kawamura, T.; Ishimoto, N.; Ito, E.
Enzymatic synthesis of uridine diphosphate N-acetyl-D-mannosaminuronic acid
J. Biol. Chem.
254
8457-8465
1979
Escherichia coli
Okubo, H.; Shibata, K.; Ishibashi, H.; Kudo, J.; Miyanaga, O.; Nagano, M.
Glucosamine-6-P synthetase and UDP-GlcNAc 2'-epimerase activities in tumor-bearing animals
Jpn. J. Clin. Chem.
8
99-103
1979
Rattus norvegicus
-
Kikuchi, K.; Tsuiki, S.
UDP-N-acetylglucosamine 2'-epimerase of rat hepatoma and its comparison with the enzyme of rat liver
Tohoku J. Exp. Med.
131
209-214
1980
Mus musculus, Rattus norvegicus
Kawamura, T.; Ishimoto, N.; Ito, E.
UDP-N-acetyl-D-glucosamine 2'-epimerase from Escherichia coli
Methods Enzymol.
83
515-519
1982
Escherichia coli
Van Rinsum, J.; van Dijk, W.; Hooghwinkel, G.J.M.; Ferwerda, W.
Subcellular localization and tissue distribution of sialic acid precursor-forming enzymes
Biochem. J.
210
21-28
1983
Rattus norvegicus
Corfield, A.P.; Clamp, J.R.; Wagner, S.A.
The metabolism of sialic acids in isolated rat colonic mucosal cells
Biochem. Soc. Trans.
11
767-768
1983
Rattus norvegicus
-
Harrington, C.R.; Baddiley, J.
Biosynthesis of wall teichoic acids in Staphylococcus aureus H, Micrococcus varians and Bacillus subtilis W23
Eur. J. Biochem.
153
639-645
1985
Kocuria varians, Staphylococcus aureus
Kawamura, T.; Ichihara, N.; Sugiyama, S.; Yokota, H.; Ishimoto, N.; Ito, E.
Biosynthesis of UDP-N-acetyl-D-glucosaminuronic acid and UDP-N-acetyl-D-mannosaminuronic acid in Micrococcus luteus
J. Biochem.
98
105-116
1985
Micrococcus luteus
Scocca, J.R.
Identification of N-acetylhexosamines produced by enzymes of the N-acetylneuraminic acid metabolic pathway by borate complex anion-exchange chromatography of the corresponding N-acetylhexosaminitols
Anal. Biochem.
156
61-66
1986
Homo sapiens
Sala, R.F.; Morgan, P.M.; Tanner, M.E.
Enzymic formation and release of a stable glycal intermediate: the mechanism of the reaction catalyzed by UDP-N-acetylglucosamine 2-epimerase
J. Am. Chem. Soc.
118
3033-3034
1996
Escherichia coli
-
Zeitler, R.; Banzer, J.P.; Bauer, C.; Reutter, W.
Inhibition of the biosynthesis of N-acetylneuraminic acid by metal ions and selenium in vitro
Biometals
5
103-109
1992
Rattus norvegicus
Gal, B.; Ruano, M.J.; Puente, R.; Garcia-Pardo, L.A.; Rueda, R.; Gil, A.; Hueso, P.
Developmental changes in UDP-N-acetylglucosamine 2-epimerase activity in rat and guinea-pig liver
Comp. Biochem. Physiol. B
118
13-15
1997
Cavia porcellus, Rattus norvegicus
Stsche, R.; Hinderlich, S.; Weise, C.; Effertz, K.; Lucka, L.; Moormann, P.; Reutter, W.
A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase
J. Biol. Chem.
272
24319-24324
1997
Rattus norvegicus (O35826)
Hinderlich, S.; Stsche, R.; Zeitler, R.; Reutter, W.
A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Purification and characterization of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
J. Biol. Chem.
272
24313-24318
1997
Rattus norvegicus
Morgan, P.M.; Sala, R.F.; Tanner, M.E.
Elimination in the reactions catalyzed by UDP-N-acetylglucosamine 2-epimerase
J. Am. Chem. Soc.
119
10269-10277
1997
Escherichia coli
-
Corfield, A.P.; Hutton, C.W.; Clamp, J.R.; Dieppe, P.A.
Changes in sialic acid metabolism occur in colon and liver during the acute-phase response
Biochem. Soc. Trans.
14
628-629
1986
Rattus norvegicus
-
Corfield, A.P.; Rainey, J.B.; Clamp, J.R.; Wagner, S.A.
Changes in the activity of the enzymes involved in sialic acid metabolism in isolated rat colonic mucosal cells on administration of azoxymethane
Biochem. Soc. Trans.
11
766-767
1983
Rattus norvegicus
-
Effertz, K.; Hinderlich, S.; Reutter, W.
Selective loss of either the epimerase or kinase activity of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase due to site-directed mutagenesis based on sequence alignments
J. Biol. Chem.
274
28771-28778
1999
Rattus norvegicus
Lucka, L.; Krause, M.; Danker, K.; Reutter, W.; Horstkorte, R.
Primary structure and expression analysis of human UDP-N-acetyl-glucosamine-2-epimerase/N-acetylmannosamine kinase, the bifunctional enzyme in neuraminic acid biosynthesis
FEBS Lett.
454
341-344
1999
Homo sapiens (Q9Y223)
Sri Kannathasan, V.; Staines, A.G.; Dong, C.J.; Field, R.A.; Preston, A.G.; Maskell, D.J.; Naismith, J.H.
Overexpression, purification, crystallization and data collection on the Bordetella pertussis wlbD gene product, a putative UDP-GlcNAc 2'-epimerase
Acta Crystallogr. Sect. D
57
1310-1312
2001
Bordetella pertussis
Campbell, R.E.; Mosimann, S.C.; Tanner, M.E.; Strynadka, N.C.
The structure of UDP-N-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferases
Biochemistry
39
14993-15001
2000
Escherichia coli (P27828)
Blume, A.; Chen, H.; Reutter, W.; Schmidt, R.R.; Hinderlich, S.
2',3'-Dialdehydo-UDP-N-acetylglucosamine inhibits UDP-N-acetylglucosamine 2-epimerase, the key enzyme of sialic acid biosynthesis
FEBS Lett.
521
127-132
2002
Rattus norvegicus
Chou, W.K.; Hinderlich, S.; Reutter, W.; Tanner, M.E.
Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase
J. Am. Chem. Soc.
125
2455-2461
2003
Rattus norvegicus
Vann, W.F.; Daines, D.A.; Murkin, A.S.; Tanner, M.E.; Chaffin, D.O.; Rubens, C.E.; Vionnet, J.; Silver, R.P.
The NeuC protein of Escherichia coli K1 is a UDP N-acetylglucosamine 2-epimerase
J. Bacteriol.
186
706-712
2004
Escherichia coli, Escherichia coli K1
Stolz, F.; Reiner, M.; Blume, A.; Reutter, W.; Schmidt, R.R.
Novel UDP-glycal derivatives as transition state analogue inhibitors of UDP-GlcNAc 2-epimerase
J. Org. Chem.
69
665-679
2004
Rattus norvegicus
Keppler, O.T.; Hinderlich, S.; Langner, J.; Schwartz-Albiez, R.; Reutter, W.; Pawlita, M.
UDP-GlcNAc 2-epimerase: a regulator of cell surface sialylation
Science
284
1372-1376
1999
Homo sapiens
Blume, A.; Weidemann, W.; Stelzl, U.; Wanker, E.E.; Lucka, L.; Donner, P.; Reutter, W.; Horstkorte, R.; Hinderlich, S.
Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Biochem. J.
384
599-607
2004
Rattus norvegicus
Murkin, A.S.; Chou, W.K.; Wakarchuk, W.W.; Tanner, M.E.
Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase
Biochemistry
43
14290-14298
2004
Neisseria meningitidis
Samuel, J.; Tanner, M.E.
Active site mutants of the "non-hydrolyzing" UDP-N-acetylglucosamine 2-epimerase from Escherichia coli
Biochim. Biophys. Acta
1700
85-91
2004
Escherichia coli
Krause, S.; Hinderlich, S.; Amsili, S.; Horstkorte, R.; Wiendl, H.; Argov, Z.; Mitrani-Rosenbaum, S.; Lochmueller, H.
Localization of UDP-GlcNAc 2-epimerase/ManAc kinase (GNE) in the Golgi complex and the nucleus of mammalian cells
Exp. Cell Res.
304
365-379
2005
Homo sapiens
Giordanengo, V.; Ollier, L.; Lanteri, M.; Lesimple, J.; March, D.; Thyss, S.; Lefebvre, J.C.
Epigenetic reprogramming of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) in HIV-1-infected CEM T cells
FASEB J.
18
1961-1963
2004
Homo sapiens (Q9Y223)
Hinderlich, S.; Salama, I.; Eisenberg, I.; Potikha, T.; Mantey, L.R.; Yarema, K.J.; Horstkorte, R.; Argov, Z.; Sadeh, M.; Reutter, W.; Mitrani-Rosenbaum, S.
The homozygous M712T mutation of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase results in reduced enzyme activities but not in altered overall cellular sialylation in hereditary inclusion body myopathy
FEBS Lett.
566
105-109
2004
Homo sapiens (Q9Y223), Homo sapiens
Sparks, S.E.; Ciccone, C.; Lalor, M.; Orvisky, E.; Klootwijk, R.; Savelkoul, P.J.; Dalakas, M.C.; Krasnewich, D.M.; Gahl, W.A.; Huizing, M.
Use of a cell-free system to determine UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities in human hereditary inclusion body myopathy
Glycobiology
15
1102-1110
2005
Homo sapiens
Noguchi, S.; Keira, Y.; Murayama, K.; Ogawa, M.; Fujita, M.; Kawahara, G.; Oya, Y.; Imazawa, M.; Goto, Y.I.; Hayashi, Y.K.; Nonaka, I.; Nishino, I.
Reduction of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase activity and sialylation in distal myopathy with rimmed vacuoles
J. Biol. Chem.
279
11402-11407
2004
Homo sapiens
Blume, A.; Benie, A.J.; Stolz, F.; Schmidt, R.R.; Reutter, W.; Hinderlich, S.; Peters, T.
Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: I. Investigation of the UDP-GlcNAc 2-epimerase functionality
J. Biol. Chem.
279
55715-55721
2004
Rattus norvegicus
Blume, A.; Ghaderi, D.; Liebich, V.; Hinderlich, S.; Donner, P.; Reutter, W.; Lucka, L.
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells
Protein Expr. Purif.
35
387-396
2004
Rattus norvegicus
Penner, J.; Mantey, L.R.; Elgavish, S.; Ghaderi, D.; Cirak, S.; Berger, M.; Krause, S.; Lucka, L.; Voit, T.; Mitrani-Rosenbaum, S.; Hinderlich, S.
Influence of UDP-GlcNAc 2-epimerase/ManNAc kinase mutant proteins on hereditary inclusion body myopathy
Biochemistry
45
2968-2977
2006
Escherichia coli (P27828), Escherichia coli
Reinke, S.O.; Hinderlich, S.
Prediction of three different isoforms of the human UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
FEBS Lett.
581
3327-3331
2007
Mus musculus (Q3UW64), Homo sapiens (Q9Y223), Homo sapiens
Bork, K.; Reutter, W.; Weidemann, W.; Horstkorte, R.
Enhanced sialylation of EPO by overexpression of UDP-GlcNAc 2-epimerase/ManAc kinase containing a sialuria mutation in CHO cells
FEBS Lett.
581
4195-4198
2007
Rattus norvegicus
Gagiannis, D.; Orthmann, A.; Danssmann, I.; Schwarzkopf, M.; Weidemann, W.; Horstkorte, R.
Reduced sialylation status in UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE)-deficient mice
Glycoconj. J.
24
125-130
2007
Mus musculus
Wang, Z.; Sun, Z.; Li, A.V.; Yarema, K.J.
Roles for UDP-GlcNAc 2-epimerase/ManNAc 6-kinase outside of sialic acid biosynthesis: modulation of sialyltransferase and BiP expression, GM3 and GD3 biosynthesis, proliferation, and apoptosis, and ERK1/2 phosphorylation
J. Biol. Chem.
281
27016-27028
2006
Homo sapiens
Ghaderi, D.; Strauss, H.M.; Reinke, S.; Cirak, S.; Reutter, W.; Lucka, L.; Hinderlich, S.
Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods
J. Mol. Biol.
369
746-758
2007
Rattus norvegicus
Castilho, A.; Pabst, M.; Leonard, R.; Veit, C.; Altmann, F.; Mach, L.; Gloessl, J.; Strasser, R.; Steinkellner, H.
Construction of a functional CMP-sialic acid biosynthesis pathway in Arabidopsis
Plant Physiol.
147
331-339
2008
Mus musculus
Velloso, L.M.; Bhaskaran, S.S.; Schuch, R.; Fischetti, V.A.; Stebbins, C.E.
A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerases. [Erratum to document cited in CA148:302197]
EMBO Rep.
9
1251
2008
Bacillus anthracis
-
Reinke, S.O.; Eidenschink, C.; Jay, C.M.; Hinderlich, S.
Biochemical characterization of human and murine isoforms of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE)
Glycoconj. J.
26
415-422
2009
Homo sapiens, Mus musculus
Namboori, S.C.; Graham, D.E.
Acetamido sugar biosynthesis in the Euryarchaea
J. Bacteriol.
190
2987-2996
2008
Methanococcus maripaludis (Q6LZC4)
Amsili, S.; Zer, H.; Hinderlich, S.; Krause, S.; Becker-Cohen, M.; MacArthur, D.G.; North, K.N.; Mitrani-Rosenbaum, S.
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) binds to alpha-actinin 1: novel pathways in skeletal muscle?
PLoS ONE
3
e2477
2008
Homo sapiens
Reinke, S.O.; Lehmer, G.; Hinderlich, S.; Reutter, W.
Regulation and pathophysiological implications of UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) as the key enzyme of sialic acid biosynthesis
Biol. Chem.
390
591-599
2009
Homo sapiens, Mus musculus, Rattus norvegicus
Voermans, N.C.; Guillard, M.; Doedee, R.; Lammens, M.; Huizing, M.; Padberg, G.W.; Wevers, R.A.; van Engelen, B.G.; Lefeber, D.J.
Clinical features, lectin staining, and a novel GNE frameshift mutation in hereditary inclusion body myopathy
Clin. Neuropathol.
29
71-77
2010
Homo sapiens
Saechao, C.; Valles-Ayoub, Y.; Esfandiarifard, S.; Haghighatgoo, A.; No, D.; Shook, S.; Mendell, J.R.; Rosales-Quintero, X.; Felice, K.J.; Morel, C.F.; Pietruska, M.; Darvish, D.
Novel GNE mutations in hereditary inclusion body myopathy patients of non-Middle Eastern descent
Genet. Test. Mol. Biomarkers
14
157-162
2010
Homo sapiens
Weidemann, W.; Klukas, C.; Klein, A.; Simm, A.; Schreiber, F.; Horstkorte, R.
Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression
Glycobiology
20
107-117
2010
Mus musculus
Kurochkina, N.; Yardeni, T.; Huizing, M.
Molecular modeling of the bifunctional enzyme UDP-GlcNAc 2-epimerase/ManNAc kinase and predictions of structural effects of mutations associated with HIBM and sialuria
Glycobiology
20
322-337
2010
Homo sapiens (Q9Y223), Homo sapiens
Moeller, H.; Boehrsch, V.; Lucka, L.; Hackenberger, C.P.; Hinderlich, S.
Efficient metabolic oligosaccharide engineering of glycoproteins by UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) knock-down
Mol. Biosyst.
7
2245-2251
2011
Homo sapiens
Xu, Y.; Brenning, B.; Clifford, A.; Vollmer, D.; Bearss, J.; Jones, C.; McCarthy, V.; Shi, C.; Wolfe, B.; Aavula, B.; Warner, S.; Bearss, D.J.; McCullar, M.V.; Schuch, R.; Pelzek, A.; Bhaskaran, S.S.; Stebbins, C.E.; Goldberg, A.R.; Fischetti, V.A.; Vankayalapati, H.
Discovery of novel putative inhibitors of UDP-GlcNAc 2-epimerase as potent antibacterial agents
ACS Med. Chem. Lett.
4
1142-1147
2013
Bacillus anthracis (Q81K32), Staphylococcus aureus (Q9REV4), Staphylococcus aureus
Chen, S.C.; Huang, C.H.; Shin Yang, C.; Liu, J.S.; Kuan, S.M.; Chen, Y.
Crystal structures of the archaeal UDP-GlcNAc 2-epimerase from Methanocaldococcus jannaschii reveal a conformational change induced by UDP-GlcNAc
Proteins
82
1519-1526
2014
Methanocaldococcus jannaschii (Q58899), Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661 (Q58899)
Zhang, L.; Muthana, M.M.; Yu, H.; McArthur, J.B.; Qu, J.; Chen, X.
Characterizing non-hydrolyzing Neisseria meningitidis serogroup A UDP-N-acetylglucosamine (UDP-GlcNAc) 2-epimerase using UDP-N-acetylmannosamine (UDP-ManNAc) and derivatives
Carbohydr. Res.
419
18-28
2016
Neisseria meningitidis serogroup A / serotype 4A (A0A0U1RGY0), Neisseria meningitidis serogroup A /serotype 4A (A0A0U1RGY0), Neisseria meningitidis serogroup A / serotype 4A Z2491 (A0A0U1RGY0), Neisseria meningitidis serogroup A /serotype 4A Z2491 (A0A0U1RGY0)
Wang, Y.T.; Missiakas, D.; Schneewind, O.
GneZ, a UDP-GlcNAc 2-epimerase, is required for S-layer assembly and vegetative growth of Bacillus anthracis
J. Bacteriol.
196
2969-2978
2014
Bacillus anthracis (Q81K32), Bacillus anthracis (Q81X16), Bacillus anthracis
Chen, S.; Huang, C.; Shin Yang, C.; Liu, J.; Kuan, S.; Chen, Y.
Crystal structures of the archaeal UDP-GlcNAc 2-epimerase from Methanocaldococcus jannaschii reveal a conformational change induced by UDP-GlcNAc
Proteins
82
1519-1526
2014
Bacillus subtilis (P39131), Bacillus subtilis, Methanocaldococcus jannaschii (Q58899), Methanocaldococcus jannaschii, Bacillus subtilis 168 (P39131)
Mann, P.A.; Mueller, A.; Wolff, K.A.; Fischmann, T.; Wang, H.; Reed, P.; Hou, Y.; Li, W.; Mueller, C.E.; Xiao, J.; Murgolo, N.; Sher, X.; Mayhood, T.; Sheth, P.R.; Mirza, A.; Labroli, M.; Xiao, L.; McCoy, M.; Gill, C.J.; Pinho, M.G.; Schneider, T.; Roemer, T.
Chemical genetic analysis and functional characterization of staphylococcal wall teichoic acid 2-epimerases reveals unconventional antibiotic drug targets
PLoS Pathog.
12
e1005585
2016
Staphylococcus epidermidis, Staphylococcus aureus (P95709), Staphylococcus aureus (Q9REV4), Staphylococcus aureus, Staphylococcus aureus COL (P95709), Staphylococcus aureus COL (Q9REV4), Staphylococcus epidermidis CLB26329
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