Information on EC 4.3.2.8 - gamma-glutamylamine cyclotransferase

for references in articles please use BRENDA:EC4.3.2.8
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.3.2.8
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RECOMMENDED NAME
GeneOntology No.
gamma-glutamylamine cyclotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
epsilon-(gamma-L-glutamyl)-L-lysine = L-lysine + 5-oxo-L-proline
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
epsilon-(gamma-L-glutamyl)-L-lysine gamma-glutamyl cyclotransferase (5-oxo-L-proline producing)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-N-acetyl-epsilon-(L-gamma-glutamyl)-L-lysine methyl ester
alpha-N-acetyl-L-lysine + 5-oxo-L-proline
show the reaction diagram
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-
-
-
?
epsilon-(gamma-L-glutamyl)-D-lysine
D-lysine + 5-oxo-L-proline
show the reaction diagram
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-
-
-
?
epsilon-(gamma-L-glutamyl)-L-lysine
L-lysine + 5-oxo-L-proline
show the reaction diagram
gamma-glutamyldansylcadaverine
dansylcadaverine + 5-oxo-L-proline
show the reaction diagram
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-
-
-
?
L-gamma-glutamyl-(2S)-2-methylbutylamine
(2S)-2-methylbutylamine + 5-oxo-L-proline
show the reaction diagram
-
-
-
?
L-gamma-glutamyl-2-methylbutylamine
2-methylbutylamine + 5-oxo-L-proline
show the reaction diagram
-
-
-
?
L-gamma-glutamyl-beta-alanine
beta-alanine + 5-oxo-L-proline
show the reaction diagram
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-
-
?
L-gamma-glutamyl-n-butylamine
n-butylamine + 5-oxo-L-proline
show the reaction diagram
L-gamma-glutamyl-n-propylamine
n-propylamine + 5-oxo-L-proline
show the reaction diagram
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-
-
?
L-gamma-glutamylbenzylamine
benzylamine + 5-oxo-L-proline
show the reaction diagram
-
-
-
?
L-gamma-glutamylcyclopentylamine
cyclopentylamine + 5-oxo-L-proline
show the reaction diagram
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-
-
?
L-gamma-glutamyldansylcadaverine
dansylcadaverine + 5-oxo-L-proline
show the reaction diagram
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-
-
-
?
L-gamma-glutamylneohexylamine
neohexylamine + 5-oxo-L-proline
show the reaction diagram
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-
-
?
L-gamma-glutamylputrescine
putrescine + 5-oxo-L-proline
show the reaction diagram
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-
-
-
?
L-gamma-glutamylspermidine
spermidine + 5-oxo-L-proline
show the reaction diagram
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-
-
-
?
Nepsilon-(L-gamma-glutamyl)-D-lysine
D-lysine + 5-oxo-L-proline
show the reaction diagram
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-
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?
Nepsilon-(L-gamma-glutamyl)-L-lysine
L-lysine + 5-oxo-L-proline
show the reaction diagram
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-
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?
S-(cyclohexylamine)-L-cysteine
?
show the reaction diagram
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-
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?
S-(n-butylcarbamyl)-L-cysteine
?
show the reaction diagram
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-
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
epsilon-(gamma-L-glutamyl)-L-lysine
L-lysine + 5-oxo-L-proline
show the reaction diagram
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-
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-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
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substantial inhibition
Cu2+
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partial inhibition
p-chloromercuribenzoate
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strong inhibition
additional information
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no inhibition by other modification reagents or other divalent cations
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.23
epsilon-(gamma-L-glutamyl)-L-lysine
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pH 7.5, 37°C
0.011
gamma-glutamyldansylcadaverine
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pH not specified in the publication, temperature not specified in the publication
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0.38
L-gamma-glutamyl-(2S)-2-methylbutylamine, L-gamma-glutamyl-2-methylbutylamine
pH not specified in the publication, temperature not specified in the publication
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0.91
L-gamma-glutamyl-beta-alanine
pH not specified in the publication, temperature not specified in the publication
0.25
L-gamma-glutamyl-n-butylamine
pH not specified in the publication, temperature not specified in the publication
0.32
L-gamma-glutamyl-n-propylamine
pH not specified in the publication, temperature not specified in the publication
0.56
L-gamma-glutamylbenzylamine
pH not specified in the publication, temperature not specified in the publication
0.084
L-gamma-glutamylcyclopentylamine
pH not specified in the publication, temperature not specified in the publication
0.011
L-gamma-glutamyldansylcadaverine
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pH 8.6, pH not specified in the publication, temperature not specified in the publication
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0.12
L-gamma-glutamylneohexylamine
pH not specified in the publication, temperature not specified in the publication
0.27
Nepsilon-(L-gamma-glutamyl)-D-lysine
pH not specified in the publication, temperature not specified in the publication
0.26
Nepsilon-(L-gamma-glutamyl)-L-lysine
pH not specified in the publication, temperature not specified in the publication
0.19
S-(cyclohexylamine)-L-cysteine
pH not specified in the publication, temperature not specified in the publication
0.091
S-(n-butylcarbamyl)-L-cysteine
pH not specified in the publication, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
L-gamma-glutamyl-(2S)-2-methylbutylamine
pH not specified in the publication, temperature not specified in the publication
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0.19
L-gamma-glutamyl-2-methylbutylamine
pH not specified in the publication, temperature not specified in the publication
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0.18
L-gamma-glutamyl-beta-alanine
pH not specified in the publication, temperature not specified in the publication
0.21
L-gamma-glutamyl-n-butylamine
pH not specified in the publication, temperature not specified in the publication
0.23
L-gamma-glutamyl-n-propylamine
pH not specified in the publication, temperature not specified in the publication
0.4
L-gamma-glutamylbenzylamine
pH not specified in the publication, temperature not specified in the publication
0.076
L-gamma-glutamylcyclopentylamine
pH not specified in the publication, temperature not specified in the publication
0.18
L-gamma-glutamylneohexylamine
pH not specified in the publication, temperature not specified in the publication
0.25
Nepsilon-(L-gamma-glutamyl)-D-lysine
pH not specified in the publication, temperature not specified in the publication
0.2
Nepsilon-(L-gamma-glutamyl)-L-lysine
pH not specified in the publication, temperature not specified in the publication
0.26
S-(cyclohexylamine)-L-cysteine
pH not specified in the publication, temperature not specified in the publication
0.27
S-(n-butylcarbamyl)-L-cysteine
pH not specified in the publication, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
L-gamma-glutamyl-(2S)-2-methylbutylamine
pH not specified in the publication, temperature not specified in the publication
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0.51
L-gamma-glutamyl-2-methylbutylamine
pH not specified in the publication, temperature not specified in the publication
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0.19
L-gamma-glutamyl-beta-alanine
pH not specified in the publication, temperature not specified in the publication
0.83
L-gamma-glutamyl-n-butylamine
pH not specified in the publication, temperature not specified in the publication
0.72
L-gamma-glutamyl-n-propylamine
pH not specified in the publication, temperature not specified in the publication
0.71
L-gamma-glutamylbenzylamine
pH not specified in the publication, temperature not specified in the publication
0.9
L-gamma-glutamylcyclopentylamine
pH not specified in the publication, temperature not specified in the publication
1.45
L-gamma-glutamylneohexylamine
pH not specified in the publication, temperature not specified in the publication
0.92
Nepsilon-(L-gamma-glutamyl)-D-lysine
pH not specified in the publication, temperature not specified in the publication
0.78
Nepsilon-(L-gamma-glutamyl)-L-lysine
pH not specified in the publication, temperature not specified in the publication
1.5
S-(cyclohexylamine)-L-cysteine
pH not specified in the publication, temperature not specified in the publication
2.8
S-(n-butylcarbamyl)-L-cysteine
pH not specified in the publication, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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protein is already present at the dormant stage and gradually activated during germination in soybean seeds
Manually annotated by BRENDA team
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
Mus musculus;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
gel fitlration
27000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structure of GGACT in complex with the reaction product 5-oxoproline
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E82A
enzyme is unstable and readily precipitates; no catalytic activity
E82Q
no catalytic activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
endpoint-assay, L-lysine is converted to alpha-keto epsilon-aminocaproate semicarbazone in the presence of semicarbazide, excess L-lysine alpha-oxidase, and catalase