T-DNA mutants of atocd and AtOCD RNAi plants have 15% higher proline accumulation at low water potential while p5cs1-4/atocd double mutants have 40% higher proline than p5cs1 at low water potential but no change in proline metabolism gene expression
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hanging-drop vapor diffusion method, crystal structure of the enzyme in complex with NADH, refined to 1.8 A resolution, crystal structure of crystals grown in presence of L-ornithine, refined to 1.6 A resolution
vapor-diffusion method, the best diffraction-quality crystals are obtained from solutions of 40%(v/v) 2-methyl-2,4-pentanediol buffered at pH 6.0 with 0.1 M MES and diffracted X-rays to 1.68 A resolution. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.0, b = 78.3, c = 119.4 A
expression of ocd from Pseudomonas putida in an ornithine overproducing platform strain with deletions of argR and argF (ORN1) from Corynebacterium glutamicum results in proline production with yields up to 0.31 g proline/g glucose