Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
biotechnology
-
bond strength of two etch-and-rinse adhesives to chondroitinase ABC treated dentin is investigated. Human extracted molars are treated with chondroitinase ABC. Increased mean values of microtensile bond strength and reduced nanoleakage expression are shown for both adhesives after chondroitinase ABC treatment of the dentin surface. This study supports the hypothesis that adhesion can be enhanced by removal of chondroitin 4/6 sulfate and dermatan sulfate, probably due to a reduced amount of water content and enlarged interfibrillar spaces
molecular biology
-
glycosaminoglycans are extracted from cooked haddock muscle. Reverse phase chromatography and digestion with chondroitinase ABC (Chase) is used. FeCl3 is mixed with the purified glycosaminoglycans, and Fe uptake is measured by ferritin formation using an in vitro digestion/Caco-2 cell model. The identificative analyses suggest that chondroitin/dermatan sulfate-related structures promote Fe uptake by Caco-2 cells
medicine
-
cervical dorsal column injury and treatment with chondroitinase ABC treatment leads to robust sprouting of both injured and intact descending projections as well as uninjured primary afferents. Enzyme treatment of uninjured animals does not induce sprouting in any system, so both denervation and chondroitin sulfate proteoglycan degradation are required to promote sprouting within the spinal cord. No increase in connectivity of nociceptive neurons or development of mechanical allodynia or thermal hyperalgesia
medicine
-
incubation of immature articular cartilage in serum-supplemented medium results in expansive growth with a marked increase in tissue volume associated with diminution of tensile integritiy. Treatment with chondroitinase ABC on day 0 leads to a marked reduction of glycosaminoglycan content and enhancement of tensile integrity. Subsequent incubation with enzyme leads to maturational growth with minimal changes in tissue volume and maintenance of tensile intregrity at the enhanced levels
medicine
-
masked intravitreal injection of chondroitinase, human or porcine plasmin into eye of pigs. Spontaneous posterior vitreous detachment occurs more frequently in human plasmin-treated and all plasmin treated eyes than in placebo controls. Treatment with chondroitinase fails to produce an effect
medicine
-
sciatic nerve transsection and end-to-end repair, with one nerve injected with chondroitinase ABC and the contralateral nerve treated with vehicle alone. Intrafascial retrograde axonal growth is equivalent in both control and chondroitinase treated conditions. Chondroitinase treatment causes a pronounced reduction in extrofacial retrograde axional growth. The decrease in axon egress from the nerve is coincident with an increase in antegrade regeneration and improved recovery of motor function
medicine
-
treatment with chondroitinase ABC improves motor function and reduces limiting ability of scar tissue to promote axonal regeneration via changing the structure of chondroitin sulfate proteoglycans
medicine
-
adult rats that undergo unilateral cervical spared-root lesion exhibit profound sensory deficits for 4 weeks after injury. Delivery of a focal intraspinal injection of the chondroitin sulfate proteoglycan-degrading enzyme chondroitinase ABC (ChABC) is sufficient to restore sensory function after lesion. In vivo electrophysiological recordings confirm that behavioral recovery observed in ChABC-treated rats is consequent on reorganization of intact C7 primary afferent terminals and not regeneration of rhizotomized afferents back into the spinal cord within adjacent segments. These data confirm that intact spinal circuits have a profound influence on functional restoration after spinal cord injury
medicine
-
antisense vimentin cDNA and ChABC are administered to hemisected rat spinal cords. Using RT-PCR, Western blotting and immunohistochemistry, it is shown that the combined treatment reduces the formation of glial scar and cystic cavities through degrading chondroitin sulfate proteoglycans molecules and inhibiting intermediate filament proteins
medicine
-
effects of degrading chondroitin sulfate glycosaminoglycan with Ch'ase ABC in the injured spinal cords of adult cats is assessed. Recovery of skilled locomotion (ladder, peg, and beam) is significantly accelerated in Ch'ase ABC-treated cats compared to controls. Ch'ase ABC-treated cats also show greater recovery of specific skilled locomotor features including intralimb movement patterns and significantly greater paw placement onto pegs. These findings show that intraspinal cleavage of CS GAGs can enhance recovery of function following spinal cord injury in large animals with sophisticated motor behaviors and axonal growth requirements similar to those encountered in humans
medicine
-
immediate and long-term effects of a single injection of chABC on chondroitin sulphate proteoglycans, chondroitin sulphate glycosaminoglycan and axon regeneration after unilateral nigrostriatal lesions in adult rats are investigated. In chABC-treated animals, the total chondroitin sulphate glycosaminoglycan remain at low level throughout the 28 day experimental period. This suggests the persistence of active chABC for at least 10 days after injection which is able to digest chondroitin sulphate proteoglycans released from cells during this time. Results suggest that a single injection of chABC can produce an environment conducive to CNS repair for over 10 days
medicine
-
it is shown that cervical spinal cord injury (SCI) results in the significantly increased expression of the chondroitin sulfate proteoglycans (CSPGs) NG2, neurocan, and brevican in the distant denervated dorsal column nuclei (DCN). The CSPGs present a potent barrier to reinnervation by regenerating axons from microtransplanted adult sensory neurons following SCI that can be overcome by chondroitinase ABC (chABC) application and increased neurotrophin-3 (NT-3) expression within the nucleus
medicine
-
rats with a crush in the dorsal funiculi of the C4 segment of the spinal cord are treated with chondroitinase ABC delivered to the lateral ventricle. Treatment is started at the time of injury or after a 2, 4 or 7 days delay. Behavioural testing over 6 weeks shows that acutely treated animals show improve skilled forelimb reaching compared to controls. Chondroitinase-treated animals show greater axon regeneration than controls. The treatment effect on contact placing, stride length and axon regeneration is not dependent on the timing of the start of treatment, but in skilled paw reaching acutely treated animals recover better function
medicine
-
topical injection of chondroitinase ABC after 15-mm tibial nerve resection can significantly increase the critical length of nerve gap repair by tubulization or artificial nerve placement
medicine
-
development of thermostabilized chondroitinase ABC and a system for its sustained local delivery in vivo, obviating the need for chronically implanted catheters and pumps. Presence of with 1M trehalose significantly enhances chondroitinase ABC thermal stability and prolongs enzyme activity. Thermostabilized chondroitinase ABC remains active at 37°C in vitro for up to 4 weeks. Chondroitin sulfate proteoglycan levels remain low in vivo up to 6 weeks post-spinal cord injury when thermostabilized chondroitin sulfate proteoglycan ABC is delivered by a hydrogel-microtube scaffold system. Axonal growth and functional recovery following the sustained local release of thermostabilized chondroitinase ABC versus a single treatment of unstabilized chondroitinase ABC demonstrate significant differences in chondroitin sulfate proteoglycan digestion. Animals treated with thermostabilized chondroitinase ABC in combination with sustained neurotrophin-3 delivery show significant improvement in locomotor function and enhanced growth of cholera toxin B subunit-positive sensory axons and sprouting of serotonergic fibers
medicine
-
intraspinal delivery of chondroitinase ABC, following T10 hemisections in adult cats, enhances adaptive movement features during a skilled locomotor task and/or promotes plasticity of spinal and supraspinal circuitry. Chondroitinase ABC enhances crossing of a peg walkway post-spinal cord injury and significantly improves ipsilateral hindlimb trajectories and integration into a functional forelimb-hindlimb coordination pattern. Recovery of these complex movements is associated with significant increases in neurofilament immunoreactivity immediately below the spinal cord injury in the ipsilateral white and contralateral gray matter. Significantly more retrogradely labeled right axotomized red nucleus neurons are seen in chondroitinase ABC-treated compared with control-treated cats indicating that a larger number of red nucleus neurons in chondroitinase ABC-treated cats had axons below the lesion level
medicine
-
role of chondroitinase ABC in the primary and secondary injury post stroke in hypertension. Renovascular hypertensive Sprague-Dawley rats underwent middle cerebral artery occlusion, and were subjected to continuous intra-infarct infusion of chondroitinase ABC for 7 days 24 h later. The intra-infarct infusion of chondroitinase ABC, by degrading accumulated chondroitin sulfate proteoglycans, rescues neuronal loss and increases the levels of growth-associated protein GAP-43 and synaptophysin in both the ipsilateral cortex and ventroposterior thalamic nucleus, indicating enhancd neuron survival as well as augmented axonal growth and synaptic plasticity, eventually improving overall neurological function
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Aguiarand, J.A.K.; Michelacci, Y.M.
Preparation and purification of Flavobacterium heparinum chondroitinases AC and B by hydrophobic interaction chromatography
Braz. J. Med. Biol. Res.
32
545-550
1999
Pedobacter heparinus
brenda
Hamai, A.; Hashimoto, N.; Mochizuki, H.; Kato, F.; Makiguchi, Y.; Horie, K.; Suzuki, S.
Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides
J. Biol. Chem.
272
9123-9130
1997
Proteus vulgaris
brenda
Asanbaeva, A.; Masuda, K.; Thonar, E.J.; Klisch, S.M.; Sah, R.L.
Mechanisms of cartilage growth. Modulation of balance between proteoglycan and collagen in vitro using chondroitinase ABC
Arthritis Rheum.
56
188-198
2007
Bos taurus
brenda
Huang, W.; Kuo, W.; Cherng, J.; Hsu, S.; Chen, P.; Huang, S.; Huang, M.; Liu, J.; Cheng, H.
Chondroitinase ABC promotes axonal re-growth and behavior recovery in spinal cord injury
Biochem. Biophys. Res. Commun.
349
963-968
2006
Rattus norvegicus
brenda
Graham, J.B.; Neubauer, D.; Xue, Q.; Muir, D.
Chondroitinase applied to peripheral nerve repair averts retrograde axonal regeneration
Exp. Neurol.
203
185-195
2007
Rattus norvegicus
brenda
Hermel, M.; Schrage, N.F.
Efficacy of plasmin enzymes and chondroitinase ABC in creating posterior vitreous separation in the pig: a masked, placebo-controlled in vivo study
Graefes Arch. Clin. Exp. Ophthalmol.
245
399-406
2007
Proteus vulgaris
brenda
Barritt, A.W.; Davies, M.; Marchand, F.; Hartley, R.; Grist, J.; Yip, P.; McMahon, S.B.; Bradbury, E.J.
Chondroitinase ABC promotes sprouting of intact and injured spinal systems after spinal cord injury
J. Neurosci.
26
10856-10867
2006
Rattus norvegicus
brenda
Tester, N.J.; Plaas, A.H.; Howland, D.R.
Effect of body temperature on chondroitinase ABCs ability to cleave chondroitin sulfate glycosaminoglycans
J. Neurosci. Res.
85
1110-1118
2007
Proteus vulgaris
brenda
Zhang, Z.; Park, Y.; Kemp, M.M.; Zhao, W.; Im, A.R.; Shaya, D.; Cygler, M.; Kim, Y.S.; Linhardt, R.J.
Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern
Anal. Biochem.
385
57-64
2009
Pedobacter heparinus, Proteus vulgaris
brenda
Xia, Y.; Zhao, T.; Li, J.; Li, L.; Hu, R.; Hu, S.; Feng, H.; Lin, J.
Antisense vimentin cDNA combined with chondroitinase ABC reduces glial scar and cystic cavity formation following spinal cord injury in rats
Biochem. Biophys. Res. Commun.
377
562-566
2008
Proteus vulgaris
brenda
Massey, J.M.; Amps, J.; Viapiano, M.S.; Matthews, R.T.; Wagoner, M.R.; Whitaker, C.M.; Alilain, W.; Yonkof, A.L.; Khalyfa, A.; Cooper, N.G.; Silver, J.; Onifer, S.M.
Increased chondroitin sulfate proteoglycan expression in denervated brainstem targets following spinal cord injury creates a barrier to axonal regeneration overcome by chondroitinase ABC and neurotrophin-3
Exp. Neurol.
209
426-445
2008
Proteus vulgaris
brenda
Tester, N.J.; Howland, D.R.
Chondroitinase ABC improves basic and skilled locomotion in spinal cord injured cats
Exp. Neurol.
209
483-496
2008
Proteus vulgaris
brenda
Garcia-Alias, G.; Lin, R.; Akrimi, S.F.; Story, D.; Bradbury, E.J.; Fawcett, J.W.
Therapeutic time window for the application of chondroitinase ABC after spinal cord injury
Exp. Neurol.
210
331-338
2008
Proteus vulgaris
brenda
Nielsen, T.C.; Meikle, P.J.; Hopwood, J.J.; Fuller, M.
Minimum substrate requirements of endoglycosidase activities toward dermatan sulfate by electrospray ionization-tandem mass spectrometry
Glycobiology
18
1119-1128
2008
Proteus vulgaris
brenda
Laparra, J.M.; Tako, E.; Glahn, R.P.; Miller, D.D.
Isolated glycosaminoglycans from cooked haddock enhance nonheme iron uptake by Caco-2 Cells
J. Agric. Food Chem.
56
10346-10351
2008
Proteus vulgaris
brenda
Mazzoni, A.; Pashley, D.H.; Ruggeri, A.J.; Vita, F.; Falconi, M.; Di Lenarda, R.; Breschi, L.
Adhesion to chondroitinase ABC treated dentin
J. Biomed. Mater. Res. B Appl. Biomater.
86B
228-236
2008
Proteus vulgaris
brenda
Hattori, T.; Matsuyama, Y.; Sakai, Y.; Ishiguro, N.; Hirata, H.; Nakamura, R.
Chondrotinase ABC enhances axonal regeneration across nerve gaps
J. Clin. Neurosci.
15
185-191
2008
Proteus vulgaris
brenda
Lin, R.; Kwok, J.C.; Crespo, D.; Fawcett, J.W.
Chondroitinase ABC has a long-lasting effect on chondroitin sulphate glycosaminoglycan content in the injured rat brain
J. Neurochem.
104
400-408
2008
Proteus vulgaris
brenda
Cafferty, W.B.; Bradbury, E.J.; Lidierth, M.; Jones, M.; Duffy, P.J.; Pezet, S.; McMahon, S.B.
Chondroitinase ABC-mediated plasticity of spinal sensory function
J. Neurosci.
28
11998-12009
2008
Proteus vulgaris
brenda
Hrabetova, S.; Masri, D.; Tao, L.; Xiao, F.; Nicholson, C.
Calcium diffusion enhanced after cleavage of negatively charged components of brain extracellular matrix by chondroitinase ABC
J. Physiol.
587
4029-4049
2009
Proteus vulgaris
brenda
Lee, H.; McKeon, R.J.; Bellamkonda, R.V.
Sustained delivery of thermostabilized chABC enhances axonal sprouting and functional recovery after spinal cord injury
Proc. Natl. Acad. Sci. USA
107
3340-3345
2010
Proteus vulgaris
brenda
Muir, E.; Fyfe, I.; Gardiner, S.; Li, L.; Warren, P.; Fawcett, J.; Keynes, R.; Rogers, J.
Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells
J. Biotechnol.
145
103-110
2010
Proteus vulgaris
brenda
Jefferson, S.C.; Tester, N.J.; Howland, D.R.
Chondroitinase ABC promotes recovery of adaptive limb movements and enhances axonal growth caudal to a spinal hemisection
J. Neurosci.
31
5710-5720
2011
Proteus vulgaris
brenda
Klueppel, M.
Efficient secretion of biologically active chondroitinase ABC from mammalian cells in the absence of an N-terminal signal peptide
Mol. Cell. Biochem.
351
1-11
2011
Proteus vulgaris (P59807), Proteus vulgaris
brenda
Nazari-Robati, M.; Khajeh, K.; Aminian, M.; Mollania, N.; Golestani, A.
Enhancement of thermal stability of chondroitinase ABC I by site-directed mutagenesis: an insight from Ramachandran plot
Biochim. Biophys. Acta
1834
479-486
2013
Proteus vulgaris (P59807)
brenda
Chen, X.R.; Liao, S.J.; Ye, L.X.; Gong, Q.; Ding, Q.; Zeng, J.S.; Yu, J.
Neuroprotective effect of chondroitinase ABC on primary and secondary brain injury after stroke in hypertensive rats
Brain Res.
1543
324-333
2014
Proteus vulgaris
brenda
Nazari-Robati, M.; Khajeh, K.; Aminian, M.; Fathi-Roudsari, M.; Golestani, A.
Co-solvent mediated thermal stabilization of chondroitinase ABC I form Proteus vulgaris
Int. J. Biol. Macromol.
50
487-492
2012
Proteus vulgaris (P59807), Proteus vulgaris
brenda
Chen, Z.; Li, Y.; Yuan, Q.
Expression, purification and thermostability of MBP-chondroitinase ABC I from Proteus vulgaris
Int. J. Biol. Macromol.
72
6-10
2014
Proteus vulgaris (P59807), Proteus vulgaris
brenda