Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
5-hydroxy-4H-benzopyran-4-one + H2O
deshydroxyvermelone
1-naphthol + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
vermelone
1,8-dihydroxynaphthalene + H2O
additional information
?
-
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
5-hydroxy-4H-benzopyran-4-one + H2O
-
-
-
r
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
5-hydroxy-4H-benzopyran-4-one + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
vermelone
1,8-dihydroxynaphthalene + H2O
-
-
-
r
vermelone
1,8-dihydroxynaphthalene + H2O
-
-
-
r
additional information
?
-
-
molecular dynamics simulation
-
-
?
additional information
?
-
-
among 1175 isolates collected from 6 prefectures of Kyushu in 2002 and 2003, 647 are resistant to MBI-D fungicides (inhibitors of melanin biosynthesis), each due to a single point mutation of the scytalone dehydratase gene. Isolates possessing the mutation in the scytalone dehydratase gene would have been selected and then multiplied rapidly in each region of Kyushu as a result of the wide-spread introduction of MBI-D fungicides in a short period
-
-
?
additional information
?
-
-
comparison of scytalone dehydratase activities between scytalone dehydratase inhibitor-sensitive and inhibitor-resistant Magnaporthe oryzae isolates overview
-
-
?
additional information
?
-
pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods
-
-
?
additional information
?
-
-
pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods
-
-
?
additional information
?
-
pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
scytalone
1,3,8-trihydroxynaphthalene + H2O
additional information
?
-
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
r
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
scytalone
1,3,8-trihydroxynaphthalene + H2O
-
-
-
?
additional information
?
-
-
among 1175 isolates collected from 6 prefectures of Kyushu in 2002 and 2003, 647 are resistant to MBI-D fungicides (inhibitors of melanin biosynthesis), each due to a single point mutation of the scytalone dehydratase gene. Isolates possessing the mutation in the scytalone dehydratase gene would have been selected and then multiplied rapidly in each region of Kyushu as a result of the wide-spread introduction of MBI-D fungicides in a short period
-
-
?
additional information
?
-
pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods
-
-
?
additional information
?
-
-
pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods
-
-
?
additional information
?
-
pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
comparison of infection rates of inhibitor MBI-D-sensitive and MBI-D-resistant isolates
evolution
phylogenetic analyses reveal that Scd1 sequences of Sclerotinia sclerotiorum and Botrytis cinerea form a monophyletic group
evolution
-
phylogenetic analyses reveal that Scd1 sequences of Sclerotinia sclerotiorum and Botrytis cinerea form a monophyletic group
-
malfunction
the sal1 mutant strain shows melanin deficiency and accumulates the intermediate scytalone, it is successfully complemented by introducing the gene fragment of Cochliobolus heterostrophus
malfunction
deficiency of the melanin biosynthesis genes SCD1 (encoding enzyme scytalone dehydratase) and THR1 (encoding a trihydroxynaphthalene reductase) affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum
malfunction
-
mutational acquisition of resistance to MBI-D negatively affects the pathogenicity of Magnaporthe oryzae due to decreased scytalone dehydratase activity
malfunction
-
deficiency of the melanin biosynthesis genes SCD1 (encoding enzyme scytalone dehydratase) and THR1 (encoding a trihydroxynaphthalene reductase) affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum
-
malfunction
-
the sal1 mutant strain shows melanin deficiency and accumulates the intermediate scytalone, it is successfully complemented by introducing the gene fragment of Cochliobolus heterostrophus
-
metabolism
-
the enzyme is involved in dihydroxynaphthalene melanin biosynthetis
metabolism
-
the enzyme is involved in melanin biosynthesis
metabolism
the enzyme is involved in melanin biosynthesis
metabolism
the enzyme is involved in melanin biosynthesis
metabolism
the enzyme is involved in melanin biosynthesis
metabolism
-
the enzyme is involved in dihydroxynaphthalene melanin biosynthetis
-
metabolism
-
the enzyme is involved in melanin biosynthesis
-
metabolism
-
the enzyme is involved in melanin biosynthesis
-
physiological function
role of the enzyme in mycelial growth, sclerotial development and fungal pathogenicity. SCD1 contributes to the initial formation of sclerotia
physiological function
-
role of the enzyme in mycelial growth, sclerotial development and fungal pathogenicity. SCD1 contributes to the initial formation of sclerotia
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
F162A
-
crystal structure
V75M
-
inhibition of the mutant enzyme by (1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide is more than 200fold reduced in comparison with that of the wild-type, about 2fold reduced turnover number for scytalone as compared to wild-type enzyme
additional information
C-terminal deletion mutants: deletion of 6 amino acids, 95% loss of activity
additional information
-
kinetic data of several point mutants
additional information
-
kinetic data of several point mutants
additional information
-
inhibitors that display variations in the hydrophilic region of the SD binding site, kinetic data for wild type and mutant enzymes
additional information
-
fungal isolates resistant to the melanin biosynthesis inhibitors-dehydratase (MBI-D), which inhibits scytalone dehydratase, are detected in Japan. The isolates have a single point mutation in the gene encoding scytalone dehydratase. In fitness tests, resistant isolates show reduced fitness, compared with that of sensitive isolates. Enzyme kinetics analyses of the scytalone dehydratases from MBI-D-sensitive and MBI-D-resistant isolates reveal that the activity of MBI-D-sensitive-type enzymes is approximately double that of MBI-D-resistant-type enzymes. The sensitive-type enzyme has higher scytalone dehydration activity than that of resistant-type enzyme at temperatures ranging from 15-35°C and at pHs ranging from pH 6.0-9.0. Acquisition of resistance to MBI-D negatively affects the pathogenicity of Magnaporthe oryzae due to decreased scytalone dehydratase activity
additional information
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods
additional information
-
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods
additional information
-
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Wheeler, M.H.; Greenblatt, G.A.
The inhibition of melanin biosynthetic reactions in Pyricularia oryzae by compounds that prevent rice blast disease
Exp. Mycol.
12
151-160
1988
Pyricularia oryzae
-
brenda
Tajima, S.; Kubo, Y.; Furusawa, I.; Shishiyama, J.
Purification of a melanin biosynthetic enzyme converting scytalone to 1,3,8-trihydroxynaphthalene from Cochliobolus miyabeanus
Exp. Mycol.
13
69-76
1989
Bipolaris oryzae
-
brenda
Butler, M.J.; Lazarovits, G.; Higgins, V.J.; Lachance, M.A.
Partial purification and characterization of a dehydratase associated with the pentaketide melanogenesis pathway of Phaeococcomyces sp. and other fungi
Exp. Mycol.
12
367-376
1988
Alternaria solani, Cladosporium cucumerinum, no activity in yeast extracts that do not form pentaketide melanins, Phaeococcomyces sp., Verticillium albo-atrum, Verticillium dahliae
-
brenda
Lundqvist, T.; Weber, P.C.; Hodge, C.N.; Braswell, E.H.; Rice, J.; Pierce, J.
Preliminary crystallographic studies on scytalone dehydratase from Magnaporthe grisea
J. Mol. Biol.
232
999-1002
1993
Pyricularia grisea
brenda
Lundqvist, T.; Rice, J.; Hodge, C.N.; Basarab, G.S.; Pierce, J.; Lindqvist, Y.
Crystal structure of scytalone dehydratase - a disease determinant of the rice pathogen, Magnaporthe grisea
Structure
2
937-944
1994
Pyricularia grisea
brenda
Basarab, G.S.; Steffens, J.J.; Wawrzak, Z.; Schwartz, R.S.; Lundqvist, T.; Jordan, D.B.
Catalytic mechanism of scytalone dehydratase: site-directed mutagenisis, kinetic isotope effects, and alternate substrates
Biochemistry
38
6012-6024
1999
Pyricularia grisea
brenda
Zheng, Y.J.; Bruice, T.C.
Role of a critical water in scytalone dehydratase-catalyzed reaction
Proc. Natl. Acad. Sci. USA
95
4158-4163
1998
Pyricularia grisea
brenda
Jordan, D.B.; Zheng, Y.J.; Lockett, B.A.; Basarab, G.S.
Stereochemistry of the enolization of scytalone by scytalone dehydratase
Biochemistry
39
2276-2282
2000
Pyricularia grisea
brenda
Jordan, D.B.; Basarab, G.S.; Steffens, J.J.; Schwartz, R.S.; Doughty, J.G.
Tight binding inhibitors of scytalone dehydratase: effects of site-directed mutations
Biochemistry
39
8593-8602
2000
Pyricularia grisea
brenda
Zheng, Y.J.; Basarab, G.S.; Jordan, D.B.
Roles of substrate distortion and intramolecular hydrogen bonding in enzymatic catalysis by scytalone dehydratase
Biochemistry
41
820-826
2002
Pyricularia grisea
brenda
Motoyama, T.; Nakasako, M.; Yamaguchi, I.
Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K
Acta Crystallogr. Sect. D
58
148-150
2002
Pyricularia oryzae
brenda
Nakasako, M.; Motoyama, T.; Kurahashi, Y.; Yamaguchi, I.
Cryogenic X-ray crystal structure analysis for the complex of scytalone dehydratase of a rice blast fungus and its tight-binding inhibitor, carpropamid: the structural basis of tight-binding inhibition
Biochemistry
37
9931-9939
1998
Pyricularia oryzae (P56221), Pyricularia oryzae
brenda
Wawrzak, Z.; Sandalova, T.; Steffens, J.J.; Basarab, G.S.; Lundqvist, T.; Lindqvist, Y.; Jordan, D.B.
High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH
Proteins
35
425-439
1999
Pyricularia grisea
brenda
Motoyama, T.; Imanishi, K.; Yamaguchi, I.
cDNA cloning, expression, and mutagenesis of scytalone dehydratase needed for pathogenicity of the rice blast fungus, Pyricularia oryzae
Biosci. Biotechnol. Biochem.
62
564-566
1998
Pyricularia grisea (P56221)
brenda
Yamada, N.; Motoyama, T.; Nakasako, M.; Kagabu, S.; Kudo, T.; Yamaguchi, I.
Enzymatic characterization of scytalone dehydratase Val75Met variant found in melanin biosynthesis dehydratase inhibitor (MBI-D) resistant strains of the rice blast fungus
Biosci. Biotechnol. Biochem.
68
615-621
2004
Pyricularia oryzae
brenda
Kihara, J.; Moriwaki, A.; Ueno, M.; Tokunaga, T.; Arase, S.; Honda, Y.
Cloning, functional analysis and expression of a scytalone dehydratase gene (SCD1) involved in melanin biosynthesis of the phytopathogenic fungus Bipolaris oryzae
Curr. Genet.
45
197-204
2004
Bipolaris oryzae
brenda
Okimoto, N.; Nakamura, T.; Suenaga, A.; Futatsugi, N.; Hirano, Y.; Yamaguchi, I.; Ebisuzaki, T.
Cooperative motions of protein and hydration water molecules: molecular dynamics study of scytalone dehydratase
J. Am. Chem. Soc.
126
13132-13139
2004
Pyricularia grisea
brenda
Suzuki, F.; Arai, M.; Yamaguchi, J.
Genetic analysis of Pyricularia grisea population by rep-PCR during development of resistance to scytalone dehydratase inhibitors of melanin biosynthesis
Plant Dis.
91
176-184
2007
Pyricularia grisea
brenda
Butler, M.J.; Gardiner, R.B.; Day, A.W.
Melanin synthesis by Sclerotinia sclerotiorum
Mycologia
101
296-304
2009
Sclerotinia sclerotiorum
brenda
Tseng, M.N.; Chung, P.C.; Tzean, S.S.
Enhancing the stress tolerance and virulence of an entomopathogen by metabolic engineering of dihydroxynaphthalene melanin biosynthesis genes
Appl. Environ. Microbiol.
77
4508-4519
2011
Alternaria alternata, Alternaria alternata BCRC30501
brenda
Suudi, M.; Park, J.M.; Kang, W.R.; Park, S.R.; Hwang, D.J.; Ahn, I.P.
Quantification of rice brown leaf spot through Taqman real-time PCR specific to the unigene encoding Cochliobolus miyabeanus scytalone dehydratase1 involved in fungal melanin biosynthesis
J. Microbiol.
50
947-954
2012
Bipolaris oryzae (Q7Z8L5), Bipolaris oryzae
brenda
Saitoh, Y.; Izumitsu, K.; Morita, A.; Shimizu, K.; Tanaka, C.
Cloning of Sal1, a scytalone dehydratase gene involved in melanin biosynthesis in Cochliobolus heterostrophus
Mycoscience
53
330-334
2012
Bipolaris maydis (A0SXQ9), Bipolaris maydis HITO7711 (A0SXQ9)
brenda
Kimura, N.; Fujimoto, H.
Comparison of scytalone dehydratase activities between scytalone dehydratase inhibitor-sensitive and inhibitor-resistant Magnaporthe oryzae isolates
J. Plant Dis. Protect.
124
525-532
2017
Pyricularia oryzae
-
brenda
Liang, Y.; Xiong, W.; Steinkellner, S.; Feng, J.
Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum
Mol. Plant Pathol.
19
1444-1453
2018
Sclerotinia sclerotiorum (A7F6T4), Sclerotinia sclerotiorum, Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 (A7F6T4)
brenda