Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(1-benzofuran-2-yl)(hydroxy)propanedioic acid + H+
(2R)-(1-benzofuran-2-yl)(hydroxy)acetic acid + CO2
93% yield
-
-
?
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
(2R)-(1-benzothiophen-5-yl)(hydroxy)acetic acid + CO2
-
-
-
?
(1-benzothiophen-6-yl)(hydroxy)propanedioic acid + H+
(2R)-(1-benzothiophen-6-yl)(hydroxy)acetic acid + CO2
89% yield
-
-
?
(1E)-but-1-en-1-yl(methyl)propanedioic acid
(2S,3Z)-2-methylhex-3-enoic acid + CO2
-
-
-
?
(4,5-dimethylfuran-2-yl)(hydroxy)propanedioic acid + H+
(2R)-(4,5-dimethylfuran-2-yl)(hydroxy)acetic acid + CO2
96% yield
-
-
?
(6-methoxynaphthalen-2-yl)(methyl)propanedioic acid
(R)-2-(6-methoxynaphthalen-2-yl)-2-methylpropanoic acid + CO2
-
-
-
?
(furan-2-yl)(hydroxy)propanedioic acid + H+
(2R)-(furan-2-yl)(hydroxy)acetic acid + CO2
95% yield
-
-
?
(R)-2-(2-naphthyl)propionate
?
(R)-2-(2-thienyl)propionate
?
(S)-2-(2-naphtyl)propionate
?
(S)-2-(2-thienyl)propionate
?
(S)-2-phenylpropionate
?
-
-
-
-
?
1-Naphthylmalonate
?
-
-
-
-
?
2(thien-2-yl) malonate
? + CO2
-
-
-
?
2-(2-fluoro-4-biphenyl)-2-methylmalonate
(R)-2-methyl-2-(2-fluoro-4-biphenyl)propionate + CO2
-
-
-
?
2-(2-fluoro-4-biphenyl)-2-methylmalonate
(R)-alpha-methyl-alpha-(2-fluoro-4-biphenyl)propionate + CO2
-
-
trivial name (R)flurbiprofen, 92% enantiomeric excess
?
2-(2-fluoro-4-biphenyl)-2-methylmalonate
(S)-2-methyl-2-(2-fluoro-4-biphenyl)propionate + CO2
-
-
-
?
2-(furan-2-yl) malonic acid
(furan-2-yl)acetic acid + CO2
-
-
-
?
2-amino-2-vinylmalonate
(R)-vinylglycine + CO2
-
-
-
?
2-aryl-2-methylmalonate
(2R)-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
2-aryl-2-methylmalonate + H+
2-arylpropionate + CO2
-
-
-
?
2-hydroxy-2-phenylmalonic acid
(2S)-hydroxy(phenyl)ethanoic acid + CO2
-
-
-
?
2-hydroxy-2-phenylmalonic acid
? + CO2
-
-
-
?
2-methyl-2-(naphthalen-2-yl)malonate
2-(naphthalen-2-yl)propionate + CO2
-
-
-
?
2-methyl-2-(thien-2-yl)malonate
2-(thien-2-yl)propionate + CO2
-
-
-
?
2-methyl-2-phenylmalonate
2-phenylpropanoate + CO2
2-methyl-2-phenylpropionate
? + CO2
-
-
-
-
?
2-methyl-2-vinylmalonic acid
(2S)-2-methylbut-3-enoic acid + CO2
for the small 2-methyl-2-vinylmalonate substrate, both the binding and the following transition states contribute to the enantioselectivity
-
-
?
2-methyl-alpha-phenylmalonate
? + CO2
-
-
-
-
?
2-Naphthylmalonate
?
-
-
-
-
?
2-phenyl-2-fluoromalonate
(R)-alpha-fluorophenylacetic acid + CO2
-
-
99% enantiomeric excess
?
2-phenyl-2-methylmalonate
(2R)-phenylpropionate + CO2
2-phenylmalonate
2-phenylacetate + CO2
2-phenylmalonate
phenylacetate + CO2
-
-
-
-
?
2-Thienylmalonate
?
-
-
-
-
?
2-vinyl-2-methylmalonic acid
(2S)-2-methylbut-3-enoic acid + CO2
-
-
-
?
2-vinyl-2-methylmalonic acid
? + CO2
-
-
-
?
alpha-aryl-alpha-methylmalonate
alpha-arylpropionate + CO2
alpha-Methyl-alpha(2-thienyl)malonate
?
-
-
-
-
?
alpha-methyl-alpha-(alpha-thienyl)malonate
alpha-(alpha-thienyl)propionate + CO2
alpha-methyl-alpha-(beta-naphthyl)malonate
alpha-(beta-naphthyl) propionate + CO2
alpha-methyl-alpha-(beta-naphthyl)malonate
alpha-(beta-naphthyl)propionate + CO2
-
-
-
-
?
alpha-Methyl-alpha-phenylmalonate
?
amino(phenyl)propanedioic acid
(2S)-amino(phenyl)ethanoic acid + CO2
-
-
-
?
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid
(2S)-2-hydroxy-4-methylpent-3-enoic acid + CO2
-
-
-
?
hydroxy(4-methylfuran-2-yl)propanedioic acid + H+
(2R)-hydroxy(4-methylfuran-2-yl)acetic acid + CO2
995% yield
-
-
?
hydroxy(5-methoxyfuran-2-yl)propanedioic acid + H+
(2R)-hydroxy(5-methoxyfuran-2-yl)acetic acid + CO2
84% yield
-
-
?
hydroxy(5-methylfuran-2-yl)propanedioic acid + H+
(2R)-hydroxy(5-methylfuran-2-yl)acetic acid + CO2
95% yield
-
-
?
hydroxy(phenyl)propanedioic acid + H+
hydroxy(phenyl)acetic acid + CO2
hydroxy(pyridin-3-yl)propanedioic acid
(2R)-hydroxy(pyridin-3-yl)acetic acid + CO2
-
-
-
?
hydroxy(pyridin-3-yl)propanedioic acid + H+
(2R)-hydroxy(pyridin-3-yl)acetic acid + CO2
97% yield
-
-
?
hydroxy(thiophen-2-yl)propanedioic acid
(2S)-hydroxy(thiophen-2-yl)acetic acid + CO2
-
-
-
?
hydroxy(thiophen-2-yl)propanedioic acid + H+
(2S)-hydroxy(thiophen-2-yl)acetic acid + CO2
97% yield
-
-
?
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid
(2S)-2-hydroxybut-3-enoic acid + CO2
-
-
-
?
m-Chlorophenylmalonate
?
-
-
-
-
?
m-Fluorophenylmalonate
?
-
-
-
-
?
mandelate
?
-
14% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
methyl(2-methylprop-1-en-1-yl)propanedioic acid
(2S)-2,4-dimethylpent-3-enoic acid + CO2
-
-
-
?
methyl(2-naphthyl)malonic acid
(2R)-2-(2-naphthyl)propanoic acid + CO2
-
-
-
-
?
methyl(2-thienyl)malonic acid
(2S)-2-(2-thienyl)propanoic acid + CO2
-
-
-
-
?
methyl(naphthalen-2-yl)propanedioic acid
(R)-2-methyl-2-(naphthalen-2-yl)propanoic acid + CO2
-
-
-
?
methyl(phenyl)propanedioate
(2R)-2-phenylpropanoate + CO2
methyl(phenyl)propanedioic acid
(2R)-2-phenylpropanoic acid + CO2
-
-
-
?
methyl(phenyl)propanedioic acid
(2S)-2-phenylpropanoic acid + CO2
-
-
-
?
methyl(thiophen-2-yl)propanedioate
(2S)-2-thiophen-2-ylpropanoate + CO2
methyl[(1E)-prop-1-en-1-yl]propanedioic acid
(2S,3Z)-2-methylpent-3-enoic acid + CO2
-
-
-
?
o-Chlorophenylmalonate
?
-
-
-
-
?
p-Chlorophenylmalonate
?
-
-
-
-
?
p-Methoxyphenylmalonate
?
phenylglycine
?
-
5% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
Phenylmalonate
Phenylacetate + CO2
[2-(2-oxoethyl)phenyl]propanedioate
2-hydroxy-2,3-dihydro-1H-indene-1-carboxylate + CO2
additional information
?
-
(R)-2-(2-naphthyl)propionate
?
-
-
-
-
?
(R)-2-(2-naphthyl)propionate
?
-
-
-
-
?
(R)-2-(2-thienyl)propionate
?
-
-
-
-
?
(R)-2-(2-thienyl)propionate
?
-
-
-
-
?
(R)-2-phenylpropionate
?
-
-
-
-
?
(R)-2-phenylpropionate
?
-
-
-
-
?
(S)-2-(2-naphtyl)propionate
?
-
-
-
-
?
(S)-2-(2-naphtyl)propionate
?
-
-
-
-
?
(S)-2-(2-thienyl)propionate
?
-
-
-
-
?
(S)-2-(2-thienyl)propionate
?
-
-
-
-
?
2-aminophenylmalonate
?
-
-
-
?
2-aminophenylmalonate
?
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
optically active
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
optically active
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-aryl-2-methylmalonate
2-arylpropionate + CO2
-
-
-
?
2-hydroxymalonate
?
-
-
-
?
2-hydroxymalonate
?
-
-
-
?
2-methyl-2-phenylmalonate
2-phenylpropanoate + CO2
-
-
-
?
2-methyl-2-phenylmalonate
2-phenylpropanoate + CO2
the enantioselectivity in the case of 2-methyl-2-phenylmalonate substrate is dictated already in the substrate binding, because only one binding mode is energetically accessible
-
-
?
2-methyl-2-phenylmalonate
2-phenylpropanoate + CO2
-
-
-
?
2-phenyl-2-methylmalonate
(2R)-phenylpropionate + CO2
-
-
-
-
?
2-phenyl-2-methylmalonate
(2R)-phenylpropionate + CO2
-
-
-
?
2-phenyl-2-methylmalonate
(2R)-phenylpropionate + CO2
-
-
more than 99% enantiomeric excess
?
2-phenyl-2-methylmalonate
(2R)-phenylpropionate + CO2
-
-
-
?
2-phenylmalonate
2-phenylacetate + CO2
-
-
-
?
2-phenylmalonate
2-phenylacetate + CO2
-
-
-
?
alpha-aryl-alpha-methylmalonate
alpha-arylpropionate + CO2
-
-
-
?
alpha-aryl-alpha-methylmalonate
alpha-arylpropionate + CO2
-
-
-
?
alpha-methyl-alpha-(alpha-thienyl)malonate
alpha-(alpha-thienyl)propionate + CO2
-
-
-
-
?
alpha-methyl-alpha-(alpha-thienyl)malonate
alpha-(alpha-thienyl)propionate + CO2
-
350% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
alpha-methyl-alpha-(alpha-thienyl)malonate
alpha-(alpha-thienyl)propionate + CO2
-
350% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
alpha-methyl-alpha-(alpha-thienyl)malonate
alpha-(alpha-thienyl)propionate + CO2
-
-
-
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
alpha-(beta-naphthyl) propionate + CO2
-
-
-
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
alpha-(beta-naphthyl) propionate + CO2
-
720% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
alpha-(beta-naphthyl) propionate + CO2
-
720% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
alpha-(beta-naphthyl) propionate + CO2
-
-
-
-
?
alpha-Methyl-alpha-phenylmalonate
?
-
-
-
-
?
alpha-Methyl-alpha-phenylmalonate
?
-
-
-
-
?
alpha-phenylbutyrate
?
-
23% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
alpha-phenylbutyrate
?
-
23% activity compared to phenylmalonate, mutant enzyme G74C
-
-
?
hydroxy(phenyl)propanedioic acid + H+
hydroxy(phenyl)acetic acid + CO2
-
-
-
?
hydroxy(phenyl)propanedioic acid + H+
hydroxy(phenyl)acetic acid + CO2
96% yield
-
-
?
methyl(phenyl)propanedioate
(2R)-2-phenylpropanoate + CO2
-
-
-
-
?
methyl(phenyl)propanedioate
(2R)-2-phenylpropanoate + CO2
-
-
-
-
?
methyl(thiophen-2-yl)propanedioate
(2S)-2-thiophen-2-ylpropanoate + CO2
-
-
-
-
?
methyl(thiophen-2-yl)propanedioate
(2S)-2-thiophen-2-ylpropanoate + CO2
-
-
-
-
?
o-Fluorophenylmalonate
?
-
-
-
-
?
o-Fluorophenylmalonate
?
-
-
-
-
?
p-Fluorophenylmalonate
?
-
-
-
-
?
p-Fluorophenylmalonate
?
-
-
-
-
?
p-Methoxyphenylmalonate
?
-
-
-
-
?
p-Methoxyphenylmalonate
?
-
-
-
-
?
p-Methylphenylmalonate
?
-
-
-
-
?
p-Methylphenylmalonate
?
-
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
phenylmalonate is phenylpropanedioate
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
phenylmalonate is phenylpropanedioate
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
best substrate
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
100% activity, mutant enzyme G74C
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
100% activity, mutant enzyme G74C
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
Phenylmalonate
Phenylacetate + CO2
-
-
-
?
[2-(2-oxoethyl)phenyl]propanedioate
2-hydroxy-2,3-dihydro-1H-indene-1-carboxylate + CO2
-
aldole reaction
-
-
?
[2-(2-oxoethyl)phenyl]propanedioate
2-hydroxy-2,3-dihydro-1H-indene-1-carboxylate + CO2
-
aldole reaction
-
-
?
additional information
?
-
-
not active with (2S)-2-methyl-2-phenylbutanoic acid
-
-
?
additional information
?
-
-
not active with (2S)-2-methyl-2-phenylbutanoic acid
-
-
?
additional information
?
-
-
mutant enzyme G74C is unable to use iso-propyl, n-propyl, as well as carboxylic acid derivatives, such as alcohol, amide, nitrile, and esters as substrates
-
-
?
additional information
?
-
2-methyl-2-(4-isobutylphenyl)malonic acid is not a substrate of wild type AMDase
-
-
?
additional information
?
-
reaction follows a mechanism in which decarboxylation of the substrate first takes place, followed by a stereoselective protonation by a cysteine residue. The enediolate intermediate and the transition states are stabilized by a number of hydrogen bonds that make up the dioxyanion hole, resulting in feasible energy barriers
-
-
?
additional information
?
-
-
the enzyme does not accept alpha,alpha-dialkyl malonic acids as substrates
-
-
?
additional information
?
-
the enzyme does not accept alpha,alpha-dialkyl malonic acids as substrates
-
-
?
additional information
?
-
-
mutant enzyme G74C is unable to use iso-propyl, n-propyl, as well as carboxylic acid derivatives, such as alcohol, amide, nitrile, and esters as substrates
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8.2
(1-benzofuran-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
0.82 - 3.1
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
3.1
(1-benzothiophen-6-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
14.4
(1E)-but-1-en-1-yl(methyl)propanedioic acid
wild type enzyme
12.7
(4,5-dimethylfuran-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
8.6
(furan-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
6.09
1-Naphthylmalonate
-
-
10.1
2-(furan-2-yl) malonic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
10.4 - 18.3
2-aryl-2-methylmalonate
4.1 - 30.2
2-hydroxy-2-phenylmalonic acid
22.2 - 26.9
2-methyl-2-phenylmalonate
19.4
2-Naphthylmalonate
-
-
9.9 - 10.7
2-phenyl-2-methylmalonate
3.3 - 7.5
2-phenylmalonate
87.4
2-Thienylmalonate
-
-
0.8 - 7.8
2-vinyl-2-methylmalonic acid
12.5
alpha-Methyl-alpha(2-thienyl)malonate
-
-
1.01 - 14.3
alpha-methyl-alpha-(alpha-thienyl)malonate
0.43 - 1.25
alpha-methyl-alpha-(beta-naphthyl)malonate
25.5
alpha-methyl-alpha-phenylmalonate
-
-
4.3 - 13.3
alpha-phenylmalonate
7.3
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid
wild type enzyme
13.3
hydroxy(4-methylfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
16.1
hydroxy(5-methoxyfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
9.8
hydroxy(5-methylfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
30.2
hydroxy(phenyl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
6.4 - 13
hydroxy(pyridin-3-yl)propanedioic acid
2.2 - 4.3
hydroxy(thiophen-2-yl)propanedioic acid
9.1
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid
wild type enzyme
9.84
m-Chlorophenylmalonate
-
-
35.7
m-Fluorophenylmalonate
-
-
3.5
methyl(2-methylprop-1-en-1-yl)propanedioic acid
wild type enzyme
0.43 - 1.56
methyl(2-naphthyl)malonic acid
5.03 - 14.5
methyl(2-thienyl)malonic acid
26.9
methyl(phenyl)propanedioic acid
wild type enzyme
12.8
methyl[(1E)-prop-1-en-1-yl]propanedioic acid
wild type enzyme
12.6
o-Chlorophenylmalonate
-
-
27.5
o-Fluorophenylmalonate
-
-
8.32
p-Chlorophenylmalonate
-
-
13.5
p-Fluorophenylmalonate
-
-
9.36
p-Methoxyphenylmalonate
-
-
8.89
p-methylphenylmalonate
-
-
0.82
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
2.1
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
3.1
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
10.4
2-aryl-2-methylmalonate
mutant enzyme G74C, pH and temperature not specified in the publication
11.4
2-aryl-2-methylmalonate
mutant enzyme G74C/V43A, pH and temperature not specified in the publication
18.3
2-aryl-2-methylmalonate
mutant enzyme G74C/M159L, pH and temperature not specified in the publication
4.1
2-hydroxy-2-phenylmalonic acid
mutant enzyme M159C
14.3
2-hydroxy-2-phenylmalonic acid
mutant enzyme G190S/P14V/P15G
15.3
2-hydroxy-2-phenylmalonic acid
mutant enzyme P14V/P15G
30.2
2-hydroxy-2-phenylmalonic acid
wild type enzyme
22.2
2-methyl-2-phenylmalonate
-
-
26.9
2-methyl-2-phenylmalonate
-
9.9
2-phenyl-2-methylmalonate
-
ybbR-tagged AMDase, in Tris buffer (0.1 M, pH 8)
10.7
2-phenyl-2-methylmalonate
-
wild type enzyme, in MOPS buffer (5-10 mM, pH 7.2)
3.3
2-phenylmalonate
mutant enzyme G74C/C188G, pH and temperature not specified in the publication
4.6
2-phenylmalonate
mutant enzyme Y48F/G74C/M159L/C188G, pH and temperature not specified in the publication
5.1
2-phenylmalonate
wild type enzyme, pH and temperature not specified in the publication
5.9
2-phenylmalonate
mutant enzyme G74C/M159L/C188G, pH and temperature not specified in the publication
7.1
2-phenylmalonate
mutant enzyme Y48F/G74C/C188G, pH and temperature not specified in the publication
7.5
2-phenylmalonate
mutant enzyme G74C/C188S, pH and temperature not specified in the publication
0.8
2-vinyl-2-methylmalonic acid
mutant enzyme G190A
7.8
2-vinyl-2-methylmalonic acid
wild type enzyme
1.01
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
3.51
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
5.03
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
5.03
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
12.5
alpha-methyl-alpha-(alpha-thienyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
12.5
alpha-methyl-alpha-(alpha-thienyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C
14.3
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
0.43
alpha-methyl-alpha-(beta-naphthyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
0.43
alpha-methyl-alpha-(beta-naphthyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C
0.51
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
0.52
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
1.11
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
1.25
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
1.25
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme G74C/S188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
4.3
alpha-phenylmalonate
-
mutant enzyme Cys101Ser
4.9
alpha-phenylmalonate
-
mutant enzyme Cys188Ser
9.1
alpha-phenylmalonate
-
mutant enzyme Cys171Ser
11.5
alpha-phenylmalonate
-
mutant enzyme Cys148Ser
13.3
alpha-phenylmalonate
-
wild-type enzyme
6.4
hydroxy(pyridin-3-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
6.4
hydroxy(pyridin-3-yl)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
7.3
hydroxy(pyridin-3-yl)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
13
hydroxy(pyridin-3-yl)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
2.2
hydroxy(thiophen-2-yl)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
3.5
hydroxy(thiophen-2-yl)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
4.3
hydroxy(thiophen-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
4.3
hydroxy(thiophen-2-yl)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
0.43
methyl(2-naphthyl)malonic acid
-
wild-type enzyme
1.25
methyl(2-naphthyl)malonic acid
-
mutant enzyme G74C/C188S
1.26
methyl(2-naphthyl)malonic acid
-
mutant enzyme C188S
1.56
methyl(2-naphthyl)malonic acid
-
mutant enzyme G74C
5.03
methyl(2-thienyl)malonic acid
-
mutant enzyme G74C/C188S
6.24
methyl(2-thienyl)malonic acid
-
mutant enzyme G74C
12.5
methyl(2-thienyl)malonic acid
-
wild-type enzyme
14.5
methyl(2-thienyl)malonic acid
-
mutant enzyme C188S
0.3
Phenylmalonate
mutant enzyme M159V
0.43
Phenylmalonate
mutant enzyme M159G
3.5
Phenylmalonate
mutant enzyme P14V/P15G
5
Phenylmalonate
mutant enzyme M159S
10.7
Phenylmalonate
wild type enzyme
12.1
Phenylmalonate
mutant enzyme M159C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
277.9
(1-benzofuran-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
54.8 - 147.7
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
147.7
(1-benzothiophen-6-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
23.9
(1E)-but-1-en-1-yl(methyl)propanedioic acid
wild type enzyme
12.4
(4,5-dimethylfuran-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
61.9
(furan-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
576
1-Naphthylmalonate
-
-
0.022 - 0.27
2-aryl-2-methylmalonate
23.4 - 101
2-hydroxy-2-phenylmalonic acid
55.8 - 279
2-methyl-2-phenylmalonate
1730
2-Naphthylmalonate
-
-
316 - 358
2-phenyl-2-methylmalonate
0.02 - 260
2-phenylmalonate
5320
2-Thienylmalonate
-
-
20.8 - 42.8
2-vinyl-2-methylmalonic acid
200
alpha-Methyl-alpha(2-thienyl)malonate
-
-
0.08 - 200
alpha-methyl-alpha-(alpha-thienyl)malonate
0.05 - 30.9
alpha-methyl-alpha-(beta-naphthyl)malonate
29.8
alpha-methyl-alpha-phenylmalonate
-
-
0.62 - 366
alpha-phenylmalonate
20.7
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid
wild type enzyme
42.4
hydroxy(4-methylfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
30.5
hydroxy(5-methoxyfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
37.1
hydroxy(5-methylfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
101
hydroxy(phenyl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
42.2 - 224.8
hydroxy(pyridin-3-yl)propanedioic acid
34 - 165.9
hydroxy(thiophen-2-yl)propanedioic acid
47.6
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid
wild type enzyme
1420
m-Chlorophenylmalonate
-
-
2180
m-Fluorophenylmalonate
-
-
5.2
methyl(2-methylprop-1-en-1-yl)propanedioic acid
wild type enzyme
0.12 - 31
methyl(2-naphthyl)malonic acid
0.34 - 200
methyl(2-thienyl)malonic acid
279
methyl(phenyl)propanedioic acid
wild type enzyme
46.2
methyl[(1E)-prop-1-en-1-yl]propanedioic acid
wild type enzyme
1090
o-Chlorophenylmalonate
-
-
666
o-Fluorophenylmalonate
-
-
782
p-Chlorophenylmalonate
-
-
195
p-Fluorophenylmalonate
-
-
92
p-Methoxyphenylmalonate
-
-
125
p-methylphenylmalonate
-
-
0.09 - 1143
Phenylmalonate
54.8
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
68.5
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
147.7
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
0.022
2-aryl-2-methylmalonate
mutant enzyme G74C/V43A, pH and temperature not specified in the publication
0.063
2-aryl-2-methylmalonate
mutant enzyme G74C/M159L, pH and temperature not specified in the publication
0.27
2-aryl-2-methylmalonate
mutant enzyme G74C, pH and temperature not specified in the publication
23.4
2-hydroxy-2-phenylmalonic acid
mutant enzyme M159C
94.4
2-hydroxy-2-phenylmalonic acid
mutant enzyme G190S/P14V/P15G
99.8
2-hydroxy-2-phenylmalonic acid
mutant enzyme P14V/P15G
101
2-hydroxy-2-phenylmalonic acid
wild type enzyme
55.8
2-methyl-2-phenylmalonate
-
-
279
2-methyl-2-phenylmalonate
-
316
2-phenyl-2-methylmalonate
-
wild type enzyme, in MOPS buffer (5-10 mM, pH 7.2)
358
2-phenyl-2-methylmalonate
-
ybbR-tagged AMDase, in Tris buffer (0.1 M, pH 8)
0.02
2-phenylmalonate
mutant enzyme G74C/C188S, pH and temperature not specified in the publication
0.05
2-phenylmalonate
mutant enzyme G74C/C188G, pH and temperature not specified in the publication
0.44
2-phenylmalonate
mutant enzyme Y48F/G74C/C188G, pH and temperature not specified in the publication
3.3
2-phenylmalonate
mutant enzyme G74C/M159L/C188G, pH and temperature not specified in the publication
11
2-phenylmalonate
mutant enzyme Y48F/G74C/M159L/C188G, pH and temperature not specified in the publication
260
2-phenylmalonate
wild type enzyme, pH and temperature not specified in the publication
20.8
2-vinyl-2-methylmalonic acid
mutant enzyme G190A
42.8
2-vinyl-2-methylmalonic acid
wild type enzyme
0.08
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
0.34
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
0.34
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
3.09
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
18.9
alpha-methyl-alpha-(alpha-thienyl)malonate
-
mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
200
alpha-methyl-alpha-(alpha-thienyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
200
alpha-methyl-alpha-(alpha-thienyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C
0.05
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
0.12
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
0.12
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme G74C/S188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C
1.14
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
3.7
alpha-methyl-alpha-(beta-naphthyl)malonate
-
mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
30.9
alpha-methyl-alpha-(beta-naphthyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C
30.9
alpha-methyl-alpha-(beta-naphthyl)malonate
-
wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C
0.62
alpha-phenylmalonate
-
mutant enzyme Cys188Ser
62.3
alpha-phenylmalonate
-
mutant enzyme Cys171Ser
100
alpha-phenylmalonate
-
mutant enzyme Cys148Ser
248
alpha-phenylmalonate
-
mutant enzyme Cys101Ser
366
alpha-phenylmalonate
-
wild-type enzyme
42.2
hydroxy(pyridin-3-yl)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
86.4
hydroxy(pyridin-3-yl)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
224.8
hydroxy(pyridin-3-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
224.8
hydroxy(pyridin-3-yl)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
34
hydroxy(thiophen-2-yl)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
79.3
hydroxy(thiophen-2-yl)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
165.9
hydroxy(thiophen-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
165.9
hydroxy(thiophen-2-yl)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
0.12
methyl(2-naphthyl)malonic acid
-
mutant enzyme G74C/C188S
0.5
methyl(2-naphthyl)malonic acid
-
mutant enzyme G74C
2.54
methyl(2-naphthyl)malonic acid
-
mutant enzyme C188S
31
methyl(2-naphthyl)malonic acid
-
wild-type enzyme
0.34
methyl(2-thienyl)malonic acid
-
mutant enzyme G74C/C188S
0.79
methyl(2-thienyl)malonic acid
-
mutant enzyme G74C
1.63
methyl(2-thienyl)malonic acid
-
mutant enzyme C188S
200
methyl(2-thienyl)malonic acid
-
wild-type enzyme
0.09
Phenylmalonate
-
246
Phenylmalonate
mutant enzyme M159G
316
Phenylmalonate
wild type enzyme
386
Phenylmalonate
mutant enzyme M159S
450
Phenylmalonate
mutant enzyme M159V
489
Phenylmalonate
mutant enzyme M159C
1143
Phenylmalonate
mutant enzyme P14V/P15G
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
33.9
(1-benzofuran-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
9.9 - 66.6
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
48.3
(1-benzothiophen-6-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
1.7
(1E)-but-1-en-1-yl(methyl)propanedioic acid
wild type enzyme
1
(4,5-dimethylfuran-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
6.4
(furan-2-yl)(hydroxy)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
0.0019 - 0.026
2-aryl-2-methylmalonate
3.3 - 6.6
2-hydroxy-2-phenylmalonic acid
29.53 - 36.16
2-phenyl-2-methylmalonate
0.0027 - 50
2-phenylmalonate
5.5 - 25.2
2-vinyl-2-methylmalonic acid
2.8
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid
wild type enzyme
3.2
hydroxy(4-methylfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
1.9
hydroxy(5-methoxyfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
3.8
hydroxy(5-methylfuran-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
3.3
hydroxy(phenyl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
3.2 - 35.3
hydroxy(pyridin-3-yl)propanedioic acid
15.2 - 38.6
hydroxy(thiophen-2-yl)propanedioic acid
5.2
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid
wild type enzyme
1.5
methyl(2-methylprop-1-en-1-yl)propanedioic acid
wild type enzyme
10.4
methyl(phenyl)propanedioic acid
wild type enzyme
3.6
methyl[(1E)-prop-1-en-1-yl]propanedioic acid
wild type enzyme
29.6 - 1499
Phenylmalonate
9.9
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
48.3
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
66.6
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
0.0019
2-aryl-2-methylmalonate
mutant enzyme G74C/V43A, pH and temperature not specified in the publication
0.0034
2-aryl-2-methylmalonate
mutant enzyme G74C/M159L, pH and temperature not specified in the publication
0.026
2-aryl-2-methylmalonate
mutant enzyme G74C, pH and temperature not specified in the publication
3.3
2-hydroxy-2-phenylmalonic acid
wild type enzyme
5.8
2-hydroxy-2-phenylmalonic acid
mutant enzyme M159C
6.5
2-hydroxy-2-phenylmalonic acid
mutant enzyme P14V/P15G
6.6
2-hydroxy-2-phenylmalonic acid
mutant enzyme G190S/P14V/P15G
29.53
2-phenyl-2-methylmalonate
-
wild type enzyme, in MOPS buffer (5-10 mM, pH 7.2)
36.16
2-phenyl-2-methylmalonate
-
ybbR-tagged AMDase, in Tris buffer (0.1 M, pH 8)
0.0027
2-phenylmalonate
mutant enzyme G74C/C188S, pH and temperature not specified in the publication
0.015
2-phenylmalonate
mutant enzyme G74C/C188G, pH and temperature not specified in the publication
0.062
2-phenylmalonate
mutant enzyme Y48F/G74C/C188G, pH and temperature not specified in the publication
0.56
2-phenylmalonate
mutant enzyme G74C/M159L/C188G, pH and temperature not specified in the publication
2.5
2-phenylmalonate
mutant enzyme Y48F/G74C/M159L/C188G, pH and temperature not specified in the publication
50
2-phenylmalonate
wild type enzyme, pH and temperature not specified in the publication
5.5
2-vinyl-2-methylmalonic acid
wild type enzyme
25.2
2-vinyl-2-methylmalonic acid
mutant enzyme G190A
3.2
hydroxy(pyridin-3-yl)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
11.8
hydroxy(pyridin-3-yl)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
35.3
hydroxy(pyridin-3-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
35.3
hydroxy(pyridin-3-yl)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
15.2
hydroxy(thiophen-2-yl)propanedioic acid
mutant P14V/P15G, pH not specified in the publication, temperature not specified in the publication
22.4
hydroxy(thiophen-2-yl)propanedioic acid
mutant M159V, pH not specified in the publication, temperature not specified in the publication
38.6
hydroxy(thiophen-2-yl)propanedioic acid
wild type enzyme, pH and temperature not specified in the publication
38.6
hydroxy(thiophen-2-yl)propanedioic acid
wild-type, pH not specified in the publication, temperature not specified in the publication
29.6
Phenylmalonate
wild type enzyme
40.4
Phenylmalonate
mutant enzyme M159C
77.2
Phenylmalonate
mutant enzyme M159S
326
Phenylmalonate
mutant enzyme P14V/P15G
570
Phenylmalonate
mutant enzyme M159G
1499
Phenylmalonate
mutant enzyme M159V
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A68C/C188S
-
no specific activity using phenylmalonate as a substrate
C101S
-
mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser. CD spectra indicate that the conformational differences of Cys101Ser and Cys188Ser compared to that of the native enzyme are not significant. Only Cys188Ser shows a drastic decrease in enzyme activity, indicating that Cys188 is located at the active centre
C148S
-
mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser. CD spectra indicate that the conformational differences of Cys101Ser and Cys188Ser compared to that of the native enzyme are not significant. Only Cys188Ser shows a drastic decrease in enzyme activity, indicating that Cys188 is located at the active centre
C171S
-
mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser. CD spectra indicate that the conformational differences of Cys101Ser and Cys188Ser compared to that of the native enzyme are not significant. Only Cys188Ser shows a drastic decrease in enzyme activity, indicating that Cys188 is located at the active centre
G190A
the mutant shows 4.6% relative activity compared to the wild type enzyme
G190S/P14V/P15G
the mutant shows 1.9% relative activity compared to the wild type enzyme
G74C/C188G
the mutant has a 5.6fold increase in activity compared with the G74C/C188S mutant
G74C/M159L
the mutant shows 14% decarboxylation activity and 165% racemisation activity compared to mutant G74C
G74C/M159L/C188G
the mutant shows a 210fold increase in activity compared with the G74C/C188S mutant
G74C/M159L/C188G/P14V/P15G
inactive
G74C/M159L/C188G/V43I/A125P/V156l
G74C/S36N/G74C/C188S
-
enhanced activity compared to G74C/C188S
G74C/V43A
the mutant with shows a 20fold shift towards promiscuous racemisation based on a reduced activity in the decarboxylation reaction and a 2fold increase in the racemisation activity. The mutant shows an extended substrate range, with a 30fold increase in the reaction rate towards ketoprofen
L72C/C188S
-
no specific activity using phenylmalonate as a substrate
L77C/C188S
-
no specific activity using phenylmalonate as a substrate
M159C
the mutant shows 1.4% relative activity compared to the wild type enzyme using phenylmalonate or methyl(phenyl)propanedioic acid as substrate, the mutant shows 1.7% relative activity compared to the wild type enzyme using 2-hydroxy-2-phenylmalonic acid as substrate
M159G
the mutant shows 19% relative activity compared to the wild type enzyme
M159S
the mutant shows 2.6% relative activity compared to the wild type enzyme
M73C/C188S
-
no specific activity using phenylmalonate as a substrate
S36N
-
activity is about 1/10 compared to that of the wild-type enzyme
S71C/C188S
-
mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme
S76C/C188S
-
no specific activity using phenylmalonate as a substrate
T75C/C188S
-
no specific activity using phenylmalonate as a substrate
V69C/C188S
-
no specific activity using phenylmalonate as a substrate
V70C/C188S
-
no specific activity using phenylmalonate as a substrate
Y48F/G74C/C188G
the mutant shows a 23fold increase in activity compared with the G74C/C188S mutant
Y48F/G74C/M159L/C188G
the mutant shows a 920fold activity increase relative to the G74C/C188S mutant
C188S
-
in contrast to the bellshaped pH profile of the wild-type enzyme, the activity of the mutant enzyme C188S retains its full activity from pH 6.0 to pH 11.0
-
A68C/C188S
-
no specific activity using phenylmalonate as a substrate
-
C188S
-
mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme
-
G74C
-
exhibits racemisation activity towards arylpropionates, in addition to its original decarboxylase activity
-
S71C/C188S
-
mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme
-
V69C/C188S
-
no specific activity using phenylmalonate as a substrate
-
C188S
-
mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser. CD spectra indicate that the conformational differences of Cys101Ser and Cys188Ser compared to that of the native enzyme are not significant. Only Cys188Ser shows a drastic decrease in enzyme activity, indicating that Cys188 is located at the active centre
C188S
-
activity with methyl(2-thienyl)malonic acid is 0.7% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 2.8% of the wild-type activity, with formation of the opposite enantiomer
C188S
in contrast to the bellshaped pH profile of the wild-type enzyme, the activity of the mutant enzyme C188S retains its full activity from pH 6.0 to pH 11.0
C188S
-
mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme
C188S
the activity of the mutant is much lower than that of the native enzyme
G74C
-
activity with methyl(2-thienyl)malonic acid is 0.79% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 0.44% of the wild-type activity, with formation of the opposite enantiomer
G74C
-
exhibits racemisation activity towards arylpropionates, in addition to its original decarboxylase activity
G74C
-
results in the formation of racemic products
G74C
the activity of the mutant is much lower than that of the native enzyme
G74C
the mutant of arylmalonate decarboxylase from Bordatella bronchoseptica has a racemising activity towards profens
G74C/C188S
-
very low activity
G74C/C188S
-
activity with methyl(2-thienyl)malonic acid is 0.42% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 0.13% of the wild-type activity, with formation of the opposite enantiomer
G74C/C188S
-
gives the opposite enantiomer of arylpropionate compared to that obtained with the wild type enzyme
G74C/C188S
-
mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme
G74C/C188S
-
results in the inversion of enantioselectivity to give (S)-alpha-arylpropionate
G74C/C188S
mutated AMDase produces arylpropionate of the opposite enantiomorph to that of the wild-type enzyme, although the enzymatic reaction proceeds with a slower rate than that of the wild type
G74C/C188S
the mutation inverts the enantioselectivity of the enzyme, the mutations have little effect on the structure of the active site
G74C/C188S
the mutant has 18000fold reduced activity compared to the wild type enzyme, the (S)-selective arylmalonate decarboxylase variant has a 220fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (99% ee)
G74C/M159L/C188G/V43I/A125P/V156l
contrary to wild-type, mutant synthesizes the (S)-enantiomer of 2-methyl-2-(2-fluoro-4-biphenyl)propionate
G74C/M159L/C188G/V43I/A125P/V156l
S-selective variant
M159V
the mutant shows 51% relative activity compared to the wild type enzyme
M159V
mutant shows increased reaction rates with furanyl malonates
M159V
the mutant has no activity with hydroxy(phenyl)propanedioic acid compared to the wild type enzyme
P14V/P15G
the mutant shows 11% relative activity compared to the wild type enzyme using phenylmalonate as substrate, the mutant shows 1.9% relative activity compared to the wild type enzyme using 2-hydroxy-2-phenylmalonic acid as substrate, the mutant shows 1.5% relative activity compared to the wild type enzyme using hydroxy(2-methylprop-1-en-1-yl)propanedioic acid as substrate
P14V/P15G
mutant displays a trend towards lower Km values than wild-type, with the entire set of malonate substrates tested
P14V/P15G
the mutant has no activity with hydroxy(phenyl)propanedioic acid compared to the wild type enzyme
S36N/G74C/C188S
-
10fold increased activity than the G74C/C188S double mutant enzyme
S36N/G74C/C188S
-
enhanced activity compared to G74C/C188S
G74C/C188S
-
gives the opposite enantiomer of arylpropionate compared to that obtained with the wild type enzyme
-
G74C/C188S
-
mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme
-
G74C/C188S
-
mutated AMDase produces arylpropionate of the opposite enantiomorph to that of the wild-type enzyme, although the enzymatic reaction proceeds with a slower rate than that of the wild type
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Miyamoto, K.; Ohta, H.
Purification and properties of a novel acylmalonate decarboxylase from Alcaligenes bronchisepticus KU 1201
Eur. J. Biochem.
210
475-481
1992
Bordetella bronchiseptica, Bordetella bronchiseptica KU 1201
brenda
Miyamoto, K.; Ohta, H.
Cloning and heterologous expression of a novel arylmalonate decarboxylase gene from Alcaligenes bronchisepticus KU 1201
Appl. Microbiol. Biotechnol.
38
234-248
1992
Bordetella bronchiseptica, Bordetella bronchiseptica KU 1201
brenda
Miyazaki, M.; Kakidani, H.; Hanzawa, S.; Ohta, H.
Cysteine 188 revealed as being critical for the enzyme acticity of arylmalonate decarboxylase by site-directed mutagenesis
Biol. Chem. Soc. Jpn.
70
2765-2769
1997
Bordetella bronchiseptica
-
brenda
Kawasaki, T.; Saito, K.; Ohta, H.
The mode of substrate-recognition mechanism of arylmalonate decarboxylase
Chem. Lett.
4
351-352
1997
Bordetella bronchiseptica
-
brenda
Fukuyama, Y.; Matoishi, K.; Iwasaki, M.; Takizawa, E.; Miyazaki, M.; Ohta, H.; Hanzawa, S.; Kakidani, H.; Sugai, T.
Preparative-scale Enzyme-catalyzed Synthesis of (R)-?-Fluorophenylacetic Acid.
Biosci. Biotechnol. Biochem.
63
1664-1666
1999
Bordetella bronchiseptica
brenda
Terao, Y.; Ijima, Y.; Kakidani, H.; Ohta, H.
Enzymatic synthesis of (R)-flurbiprofen
Bull. Chem. Soc. JPN
76
2395-2397
2003
Bordetella bronchiseptica
-
brenda
Matoishi, K.; Kakidani, H.; Suzuki, M.; Sugai, T.; Ohta, H.; Hanzawa, S.
The first synthesis of both enantiomers of [alpha-2H]phenylacetic acid in high enantiomeric excess
Chem. Commun. (Camb.)
2000
1519-1520
2000
Bordetella bronchiseptica
-
brenda
Ijima, Y.; Matoishi, K.; Terao, Y.; Doi, N.; Yanagawa, H.; Ohta, H.
Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism
Chem. Commun. (Camb.)
2005
877-879
2005
Bordetella bronchiseptica
brenda
Matoishi, K.; Ueda, M.; Miyamoto, K.; Ohta, H.
Mechanism of asymmetric decarboxylation of alpha-aryl-alpha-methylmalonate catalyzed by arylmalonate decarboxylase originated from Alcaligenes bronchisepticus
J. Mol. Catal. B
27
161-168
2004
Bordetella bronchiseptica (Q05115), Bordetella bronchiseptica KU 1201 (Q05115)
-
brenda
Terao, Y.; Miyamoto, K.; Ohta, H.
Improvement of the activity of arylmalonate decarboxylase by random mutagenesis
Appl. Microbiol. Biotechnol.
73
647-653
2006
Bordetella bronchiseptica
brenda
Terao, Y.; Miyamoto, K.; Ohta, H.
Introduction of single mutation changes arylmalonate decarboxylase to racemase
Chem. Commun. (Camb. )
34
3600-3602
2006
Bordetella bronchiseptica, Bordetella bronchiseptica KU1201
brenda
Terao, Y.; Miyamoto, K.; Ohta, H.
The aldol type reaction catalyzed by arylmalonate decarboxylase - a decarboxylase can catalyze an entirely different reaction, aldol reaction -
Chem. Lett.
36
420-421
2007
Bordetella bronchiseptica, Bordetella bronchiseptica KU1201
-
brenda
Miyamoto, K.; Tsutsumi, T.; Terao, Y.; Ohta, H.
Stereochemistry of decarboxylation of arylmalonate catalyzed by mutant enzymes
Chem. Lett.
36
656-657
2007
Bordetella bronchiseptica
-
brenda
Miyamoto, K.; Yatake, Y.; Tamura, K.; Terao, Y.; Ohta, H.
Purification and characterization of arylmalonate decarboxylase from Achromobacter sp. KU1311
J. Biosci. Bioeng.
104
263-267
2007
Achromobacter sp., Achromobacter sp. KU1311
brenda
Terao, Y.; Ijima, Y.; Miyamoto, K.; Ohta, H.
Inversion of enantioselectivity of arylmalonate decarboxylase via site-directed mutation based on the proposed reaction mechanism
J. Mol. Catal. B
45
15-20
2007
Bordetella bronchiseptica, Bordetella bronchiseptica KU1201
-
brenda
Nakasako, M.; Obata, R.; Okubo, R.; Nakayama, S.; Miyamoto, K.; Ohta, H.
Crystallization and preliminary X-ray diffraction experiments of arylmalonate decarboxylase from Alcaligenes bronchisepticus
Acta Crystallogr. Sect. F
64
610-613
2008
Bordetella bronchiseptica (Q05115), Bordetella bronchiseptica, Bordetella bronchiseptica KU1201 (Q05115)
brenda
Yatake, Y.; Miyamoto, K.; Ohta, H.
Screening, cloning, expression, and purification of an acidic arylmalonate decarboxylase from Enterobacter cloacae KU1313
Appl. Microbiol. Biotechnol.
78
793-799
2008
Enterobacter cloacae, Enterobacter cloacae KU1313
brenda
Tamura, K.; Terao, Y.; Miyamoto, K.; Ohta, H.
Asymmetric decarboxylation of alpha -hydroxy- and alpha -amino-alpha -phenylmalonate catalyzed by arylmalonate decarboxylase from Alcaligenes bronchisepticus
Biocatal. Biotransform.
26
253-257
2008
Bordetella bronchiseptica (Q05115), Bordetella bronchiseptica KU 1201 (Q05115)
-
brenda
Okrasa, K.; Levy, C.; Hauer, B.; Baudendistel, N.; Leys, D.; Micklefield, J.
Structure and mechanism of an unusual malonate decarboxylase and related racemases
Chem. Eur. J.
14
6609-6613
2008
Chelativorans sp. BNC1, Alkaliphilus metalliredigens QYMF, Bordetella bronchiseptica (A0A0H3LJU9), Bordetella bronchiseptica (Q05115), Pyrococcus horikoshii (O58713), Bordetella bronchiseptica KU1201 (Q05115), Bordetella bronchiseptica RB50 (A0A0H3LJU9), Pyrococcus horikoshii OT-3 (O58713)
brenda
Kuettner, E.B.; Keim, A.; Kircher, M.; Rosmus, S.; Straeter, N.
Active-site mobility revealed by the crystal structure of arylmalonate decarboxylase from Bordetella bronchiseptica
J. Mol. Biol.
377
386-394
2008
Bordetella bronchiseptica (Q05115), Bordetella bronchiseptica
brenda
Okrasa, K.; Levy, C.; Wilding, M.; Goodall, M.; Baudendistel, N.; Hauer, B.; Leys, D.; Micklefield, J.
Structure-guided directed evolution of alkenyl and arylmalonate decarboxylases
Angew. Chem. Int. Ed. Engl.
48
7691-7694
2009
Bordetella bronchiseptica (Q05115)
brenda
Obata, R.; Nakasako, M.
Structural basis for inverting the enantioselectivity of arylmalonate decarboxylase revealed by the structural analysis of the Gly74Cys/Cys188Ser mutant in the liganded form
Biochemistry
49
1963-1969
2010
Bordetella bronchiseptica (Q05115)
brenda
Wong, L.S.; Okrasa, K.; Micklefield, J.
Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles
Org. Biomol. Chem.
8
782-787
2010
Bordetella bronchiseptica
brenda
Miyauchi, Y.; Kourist, R.; Uemura, D.; Miyamoto, K.
Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution
Chem. Commun. (Camb. )
47
7503-7505
2011
Bordetella bronchiseptica (Q05115)
brenda
Kourist, R.; Miyauchi, Y.; Uemura, D.; Miyamoto, K.
Engineering the promiscuous racemase activity of an arylmalonate decarboxylase
Chemistry
17
557-563
2011
Bordetella bronchiseptica (Q05115)
brenda
Lind, M.; Himo, F.
Theoretical study of reaction mechanism and stereoselectivity of arylmalonate decarboxylase
ACS Catal.
4
4153-4160
2014
Bordetella bronchiseptica (Q05115)
-
brenda
Gassmeyer, S.; Wetzig, J.; Mgge, C.; Assmann, M.; Enoki, J.; Hilterhaus, L.; Zuhse, R.; Miyamoto, K.; Liese, A.; Kourist, R.
Arylmalonate decarboxylase-catalyzed asymmetric synthesis of both enantiomers of optically pure flurbiprofen
ChemCatChem
8
916-921
2016
Bordetella bronchiseptica (Q05115)
-
brenda
Lewin, R.; Goodall, M.; Thompson, M.L.; Leigh, J.; Breuer, M.; Baldenius, K.; Micklefield, J.
Enzymatic enantioselective decarboxylative protonation of heteroaryl malonates
Chemistry
21
6557-6563
2015
Bordetella bronchiseptica, Bordetella bronchiseptica (Q05115)
brenda
Assmann, M.; Muegge, C.; Gassmeyer, S.K.; Enoki, J.; Hilterhaus, L.; Kourist, R.; Liese, A.; Kara, S.
Improvement of the process stability of arylmalonate decarboxylase by immobilization for biocatalytic profen synthesis
Front. Microbiol.
8
448
2017
Bordetella bronchiseptica (Q05115)
brenda