Reference on EC 3.5.5.1 - nitrilase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Levy-Schil, S.; Soubrier, F.; Crutz-Le Coq, A.M.; Faucher, D.; Crouzet, J.; Petre, D.
Aliphatic nitrilase from a soil-isolated Comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure
Gene
161
15-20
1995
Comamonas testosteroni (Q59329), Comamonas testosteroni
Nagawasa, T.; Wieser, M.; Nakamura, T.; Iwahara, H.; Yoshida, T.; Gekko, K.
Nitrilase of Rhodococcus rhodochrous J1. Conversion into active form by subunit association
Eur. J. Biochem.
267
138-144
2000
Rhodococcus rhodochrous
Grsic-Rausch, S.; Kobelt, P.; Siemens, J.M.; Bischoff, M.; Ludwig-Muller, J.
Expression and localization of nitrilase during symptom development of the clubroot disease in Arabidopsis
Plant Physiol.
122
369-378
2000
Arabidopsis thaliana
Almatawah, Q.A.; Cowan, D.A.
Thermostable nitrilase catalysed production of nicotinic acid from 3-cyanopyridine
Enzyme Microb. Technol.
25
718-724
1999
Aeribacillus pallidus
-
Almatawah, Q.A.; Cramp, R.; Cowan, D.A.
Characterization of an inducible nitrilase from a thermophilic bacillus
Extremophiles
3
283-291
1999
Aeribacillus pallidus
Dhillon, J.; Chhatre, S.; Shanker, R.; Shivaraman, N.
Transformation of aliphatic and aromatic nitriles by a nitrilase from Pseudomonas sp.
Can. J. Microbiol.
45
811-815
1999
Pseudomonas sp.
-
Layh, N.; Willetts, A.
Enzymatic nitrile hydrolysis in low water systems
Biotechnol. Lett.
20
329-331
1998
Pseudomonas sp.
-
Kobayashi, M.; Goda, M.; Shimizu, S.
Nitrilase catalyzes amide hydrolysis as well as nitrile hydrolysis
Biochem. Biophys. Res. Commun.
253
662-666
1998
Rhodococcus rhodochrous
Bartling, D.; Seedorf, M.; Schmidt, R.C.; Weiler, E.W.
Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: Key enzymes in biosynthesis of the plant hormone indole-3-acetic acid
Proc. Natl. Acad. Sci. USA
91
6021-6025
1994
Arabidopsis thaliana
Kobayashi, M.; Komeda, H.; Yanaka, N.; Nagasawa, T.; Yamada, H.
Nitrilase from Rhodococcus rhodochrous J1. Sequencing and overexpression of the gene and identification of an essential cysteine residue
J. Biol. Chem.
267
20746-20751
1992
Rhodococcus rhodochrous
Bartling, D.; Seedorf, M.; Mithfer, A.; Weiler, E.W.
Cloning and expression of an Arabidopsis nitrilase which can convert indole-3-acetonitrile to the plant hormone, indole-3-acetic acid
Eur. J. Biochem.
205
417-424
1992
Arabidopsis thaliana
Stevenson, D.E.; Feng, R.; Dumas, F.; Groleau, D.; Mihoc, A.; Storer, A.C.
Mechanistic and structural studies on Rhodococcus ATCC 39484 nitrilase
Biotechnol. Appl. Biochem.
15
283-302
1992
Rhodococcus sp.
Bhalla, T.C.; Miura, A.; Wakamoto, A.; Ohba, Y.; Furuhashi, K.
Asymmetric hydrolysis of alpha-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34
Appl. Microbiol. Biotechnol.
37
184-190
1992
Rhodococcus rhodochrous
-
Stalker, D.M.; McBride, K.E.
Cloning and expression in Escherichia coli of a Klebsiella ozaenae plasmid-borne gene encoding a nitrilase specific for the herbicide bromoxynil
J. Bacteriol.
169
955-960
1987
Klebsiella pneumoniae subsp. ozaenae
McBride, K.E.; Kenny, J.W.; Stalker, D.M.
Metabolism of the herbicide bromoxynil by Klebsiella pneumoniae subsp. ozaenae
Appl. Environ. Microbiol.
52
325-330
1986
Klebsiella pneumoniae subsp. ozaenae
Bandypadhyay, A.K.; Nagasawa, T.; Asano, Y.; Fujishiro, K.; Tani, Y.; Yamada, H.
Purification and characterization of benzonitrilases from Arthrobacter sp. strain J-1
Appl. Environ. Microbiol.
51
302-306
1986
Arthrobacter sp., Arthrobacter sp. J-1
Kuwahara, M.; Yanase, H.; Ishida, Y.; Kikuchi, Y.
Metabolism of aliphatic nitriles in Fusarium solani
J. Ferment. Technol.
58
573-577
1980
Fusarium solani
-
Rausch, T.; Hilgenberg, W.
Partial purification of nitrilase from Chinese cabbage
Phytochemistry
19
747-750
1980
Brassica rapa subsp. pekinensis
-
Harper, D.B.
Fungal degradation of aromatic nitriles. Enzymology of C-N cleavage by Fusarium solani
Biochem. J.
167
685-692
1977
Fusarium solani
Harper, D.B.
Microbial metabolism of aromatic nitriles. Enzymology of C-N cleavage by Nocardia sp. (Rhodococcus group) N.C.I.B. 11216
Biochem. J.
165
309-319
1977
Nocardia sp.
Harper, D.B.
Purification and properties of an unusual nitrilase from Nocardia N.C.I.B. 11216
Biochem. Soc. Trans.
4
502-504
1976
Nocardia sp.
Thimann, K.W.; Mahadevan, S.
Nitrilase. I. Occurrence, preparation, and general properties of the enzyme
Arch. Biochem. Biophys.
105
133-141
1964
Hordeum vulgare, no activity in Allium cepa, no activity in Citrus limon, no activity in Croton sp., no activity in Cucumis sativus, no activity in Ginkgo biloba, no activity in Helianthus annuus, no activity in Heliconia sp., no activity in Impatiens balsamina, no activity in Iris sp., no activity in Kalanchoe blossfeldiana, no activity in Lilium sp., no activity in Solanum lycopersicum, no activity in Monstera deliciosa, no activity in Nerium oleander, no activity in Onoclea sensibilis, no activity in Pelargonium zonale, no activity in Pteridium aquilinum, no activity in Rosa sp., no activity in Tradescantia virginiana, no activity in Xanthium pennsylvanicum
O'Reilly, C.; Turner, P.D.
The nitrilase family of CN hydrolyzing enzymes - a comparative study
J. Appl. Microbiol.
95
1161-1174
2003
Acinetobacter sp., Alcaligenes faecalis, Arabidopsis thaliana, Aeribacillus pallidus, Bacillus sp. (in: Bacteria), Brassica rapa, Comamonas testosteroni, Fusarium oxysporum, Fusarium solani, Hordeum vulgare, Acidovorax facilis, Klebsiella pneumoniae, Nocardia sp., Pseudomonas sp., Rhodococcus rhodochrous, Rhodococcus sp., Fusarium solani IMI196840, Rhodococcus sp. ATCC 39484, Acinetobacter sp. AK226, Bacillus sp. (in: Bacteria) OxB-1, Rhodococcus rhodochrous K22, Alcaligenes faecalis JM3, Aeribacillus pallidus Dac521, Acidovorax facilis 72W
Brady, D.; Beeton, A.; Zeevaart, J.; Kgaje, C.; van Rantwijk, F.; Sheldon, R.A.
Characterisation of nitrilase and nitrile hydratase biocatalytic systems
Appl. Microbiol. Biotechnol.
64
76-85
2004
Rhodococcus sp.
Brenner, C.
Catalysis in the nitrilase superfamily
Curr. Opin. Struct. Biol.
12
775-782
2002
Arabidopsis thaliana (P32961), Homo sapiens (Q86X76)
Vorwerk, S.; Biernacki, S.; Hillebrand, H.; Janzik, I.; Muller, A.; Weiler, E.W.; Piotrowski, M.
Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster
Planta
212
508-516
2001
Arabidopsis thaliana (P32961), Arabidopsis thaliana (P32962), Arabidopsis thaliana (P46010), Arabidopsis thaliana
Wieser, M.; Heinzmann, K.; Kiener, A.
Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336
Appl. Microbiol. Biotechnol.
48
174-180
1997
Agrobacterium sp.
-
Petersen, M.; Kiener, A.
Biocatalysis - preparation and functionalization of N-heterocycles
Green Chem.
2
99-106
1999
Alcaligenes faecalis
-
Banerjee, A.; Kaul, P.; Banerjee, U.C.
Enhancing the catalytic potential of nitrilase from Pseudomonas putida for stereoselective nitrile hydrolysis
Appl. Microbiol. Biotechnol.
72
77-87
2006
Pseudomonas putida
Mustacchi, R.; Knowles, C.J.; Li, H.; Dalrymple, I.; Sunderland, G.; Skibar, W.; Jackman, S.A.
The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal
Biotechnol. Bioeng.
91
436-440
2005
Rhodococcus rhodochrous
Vejvoda, V.; Kaplan, O.; Klozova, J.; Masak, J.; Cejkova, A.; Jirku, V.; Stloukal, R.; Martinkova, L.
Mild hydrolysis of nitriles by Fusarium solani strain O1
Folia Microbiol. (Praha)
51
251-256
2006
Fusarium solani, Fusarium solani O1
Kabaivanova, L.; Dobreva, E.; Dimitrov, P.; Emanuilova, E.
Immobilization of cells with nitrilase activity from a thermophilic bacterial strain
J. Ind. Microbiol. Biotechnol.
32
7-11
2005
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) UG-5B
Yildirim, S.; Ruinatscha, R.; Gross, R.; Wohlgemuth, R.; Kohler, H.E.; Witholt, B.; Schmid, A.
Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles
J. Mol. Catal. B
38
76-83
2006
Arabidopsis thaliana, Rhodococcus sp.
-
Kamila, S.; Zhu, D.; Biehl, E.R.; Hua, L.
Unexpected stereorecognition in nitrilase-catalyzed hydrolysis of beta-hydroxy nitriles
Org. Lett.
8
4429-4431
2006
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
Dixon, D.P.; Skipsey, M.; Grundy, N.M.; Edwards, R.
Stress-induced protein S-glutathionylation in Arabidopsis
Plant Physiol.
138
2233-2244
2005
Arabidopsis thaliana (P32961)
Fernandes, B.C.; Mateo, C.; Kiziak, C.; Chmura, A.; Wacker, J.; van Rantwijk, F.; Stolz, A.; Sheldon, R.A.
Nitrile hydratase activity of a recombinant nitrilase
Adv. Synth. Catal.
348
2597-2603
2006
Pseudomonas fluorescens, Pseudomonas fluorescens EBC 191
-
Zhu, D.; Mukherjee, C.; Biehl, E.R.; Hua, L.
Nitrilase-catalyzed selective hydrolysis of dinitriles and green access to the cyanocarboxylic acids of pharmaceutical importance
Adv. Synth. Catal.
349
1667-1670
2007
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
-
Kaul, P.; Stolz, A.; Banerjee, U.C.
Cross-linked amorphous nitrilase aggregates for enantioselective nitrile hydrolysis
Adv. Synth. Catal.
349
2167-2176
2007
Alcaligenes faecalis
-
Zhu, Q.; Fan, A.; Wang, Y.; Zhu, X.; Wang, Z.; Wu, M.; Zheng, Y.
Novel sensitive high-throughput screening strategy for nitrilase-producing strains
Appl. Environ. Microbiol.
73
6053-6057
2007
Bacillus subtilis, Rhodococcus equi, Bacillus subtilis E9, Rhodococcus equi CCTCC.M.205114
Kaplan, O.; Vejvoda, V.; Plihal, O.; Pompach, P.; Kavan, D.; Bojarova, P.; Bezouska, K.; Mackova, M.; Cantarella, M.; Jirku, V.; Kren, V.; Martinkova, L.
Purification and characterization of a nitrilase from Aspergillus niger K10
Appl. Microbiol. Biotechnol.
73
567-575
2006
Aspergillus niger, Aspergillus niger K10
Yeom, S.J.; Kim, H.J.; Lee, J.K.; Kim, D.E.; Oh, D.K.
A determinant residue of substrate specificity in nitrilase from Rhodococcus rhodochrous ATCC 33278 for aliphatic and aromatic nitriles
Biochem. J.
415
401-407
2008
Rhodococcus rhodochrous
Holtze, M.S.; Sorensen, J.; Hansen, H.C.; Aamand, J.
Transformation of the herbicide 2,6-dichlorobenzonitrile to the persistent metabolite 2,6-dichlorobenzamide (BAM) by soil bacteria known to harbour nitrile hydratase or nitrilase
Biodegradation
17
503-510
2006
Pseudomonas putida, Pseudomonas fluorescens, Rhizobium sp., Pseudomonas putida 11388, Pseudomonas fluorescens 11387, Rhizobium sp. 11401
Naik, S.C.; Kaul, P.; Barse, B.; Banerjee, A.; Banerjee, U.C.
Studies on the production of enantioselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110
Biores. Technol.
99
26-31
2008
Pseudomonas putida, Pseudomonas putida MTCC 5110
Wu, S.; Fogiel, A.J.; Petrillo, K.L.; Hann, E.C.; Mersinger, L.J.; DiCosimo, R.; OKeefe, D.P.; Ben-Bassat, A.; Payne, M.S.
Protein engineering of Acidovorax facilis 72W nitrilase for bioprocess development
Biotechnol. Bioeng.
97
689-693
2007
Acidovorax facilis, Acidovorax facilis 72W
Wu, S.; Fogiel, A.J.; Petrillo, K.L.; Jackson, R.E.; Parker, K.N.; DiCosimo, R.; Ben-Bassat, A.; OKeefe, D.P.; Payne, M.S.
Protein engineering of nitrilase for chemoenzymatic production of glycolic acid
Biotechnol. Bioeng.
99
717-720
2007
Acidovorax facilis, Acidovorax facilis 72W
Vejvoda, V.; Sveda, O.; Kaplan, O.; Prikrylova, V.; Elisakova, V.; Himl, M.; Kubac, D.; Pelantova, H.; Kuzma, M.; Kren, V.; Martinkova, L.
Biotransformation of heterocyclic dinitriles by Rhodococcus erythropolis and fungal nitrilases
Biotechnol. Lett.
29
1119-1124
2007
Fusarium solani
Singh, R.; Sharma, R.; Tewari, N.; Tewari, N.; Rawat, D.S.
Nitrilase and its application as a green catalyst
Chem. Biodivers.
3
1279-1287
2006
Acinetobacter sp., Alcaligenes faecalis, Arabidopsis thaliana, Aeribacillus pallidus, Bacillus sp. (in: Bacteria), Comamonas testosteroni, Fusarium oxysporum, Fusarium solani, Hordeum vulgare, Acidovorax facilis, Klebsiella pneumoniae, Penicillium multicolor, Pseudomonas sp., Rhodococcus rhodochrous, Rhodococcus sp., Fusarium solani IMI196840, Acinetobacter sp. AK226, Bacillus sp. (in: Bacteria) OxB-1, Rhodococcus rhodochrous K22, Aeribacillus pallidus Dac521, Acidovorax facilis 72W
He, Y.C.; Xu, J.H.; Xu, Y.; Ouyang, L.M.; Pan, J.
Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp. ECU0401
Chin. Chem. Lett.
18
677-680
2007
Alcaligenes sp., Alcaligenes sp. ECU0401
-
Rustler, S.; Mueller, A.; Windeisen, V.; Chmura, A.; Fernandes, B.C.; Kiziak, C.; Stolz, A.
Conversion of mandelonitrile and phenylglycinenitrile by recombinant E. coli cells synthesizing a nitrilase from Pseudomonas fluorescens EBC191
Enzyme Microb. Technol.
40
598-606
2007
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
-
Mukherjee, C.; Zhu, D.; Biehl, E.R.; Hua, L.
Exploring the synthetic applicability of a cyanobacterium nitrilase as catalyst for nitrile hydrolysis
Eur. J. Org. Chem.
23
5238-5242
2006
Synechocystis sp.
-
Thuku, R.N.; Weber, B.W.; Varsani, A.; Sewell, B.T.
Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form
FEBS J.
274
2099-2108
2007
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
Kriechbaumer, V.; Park, W.J.; Piotrowski, M.; Meeley, R.B.; Gierl, A.; Glawischnig, E.
Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis
J. Exp. Bot.
58
4225-4233
2007
Zea mays
Yazbeck, D.R.; Durao, P.J.; Xie, Z.; Tao, J.
A metal ion-based method for the screening of nitrilases
J. Mol. Catal. B
39
156-159
2006
Arabidopsis thaliana
-
Xie, Z.; Feng, J.; Garcia, E.; Bernett, M.; Yazbeck, D.; Tao, J.
Cloning and optimization of a nitrilase for the synthesis of (3S)-3-cyano-5-methyl hexanoic acid
J. Mol. Catal. B
41
75-80
2006
Arabidopsis thaliana
-
Vejvoda, V.; Kaplan, O.; Bezouska, K.; Pompach, P.; Sulc, M.; Cantarella, M.; Benada, O.; Uhnakova, B.; Rinagelova, A.; Lutz-Wahl, S.; Fischer, L.; Kren, V.; Martinkova, L.
Purification and characterization of a nitrilase from Fusarium solani O1
J. Mol. Catal. B
50
99-106
2008
Fusarium solani, Fusarium solani O1
-
Ishikawa, T.; Okazaki, K.; Kuroda, H.; Itoh, K.; Mitsui, T.; Hori, H.
Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development
Mol. Plant Pathol.
8
623-637
2007
Brassica rapa (A3QYW3), Brassica rapa
Jenrich, R.; Trompetter, I.; Bak, S.; Olsen, C.E.; Moller, B.L.; Piotrowski, M.
Evolution of heteromeric nitrilase complexes in Poaceae with new functions in nitrile metabolism
Proc. Natl. Acad. Sci. USA
104
18848-18853
2007
Sorghum bicolor
Banerjee, A.; Dubey, S.; Kaul, P.; Barse, B.; Piotrowski, M.; Banerjee, U.C.
enantioselective nitrilase from Pseudomonas putida: cloning, heterologous expression, and bioreactor studies
Mol. Biotechnol.
41
35-41
2008
Pseudomonas putida
Luo, H.; Fan, L.; Chang, Y.; Ma, J.; Yu, H.; Shen, Z.
Gene cloning, overexpression, and characterization of the nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli
Appl. Biochem. Biotechnol.
160
393-400
2008
Rhodococcus rhodochrous (A4LA85), Rhodococcus rhodochrous tg1-A6 (A4LA85), Rhodococcus rhodochrous tg1-A6
Chmura, A.; Shapovalova, A.A.; van Pelt, S.; van Rantwijk, F.; Tourova, T.P.; Muyzer, G.; Sorokin, D.Y.
Utilization of arylaliphatic nitriles by haloalkaliphilic Halomonas nitrilicus sp. nov. isolated from soda soils
Appl. Microbiol. Biotechnol.
81
371-378
2008
Halomonas nitrilica
Barglow, K.T.; Saikatendu, K.S.; Bracey, M.H.; Huey, R.; Morris, G.M.; Olson, A.J.; Stevens, R.C.; Cravatt, B.F.
Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes
Biochemistry
47
13514-13523
2008
Mus musculus
Zhang, H.; Hou, Y.J.; Han, S.Y.; Zhang, E.C.; Huebner, K.; Zhang, J.
Mammalian nitrilase 1 homologue Nit1 is a negative regulator in T cells
Int. Immunol.
21
691-703
2009
Mus musculus
Kaul, P.; Banerjee, U.C.
Predicting enzyme behavior in nonconventional media: correlating nitrilase function with solvent properties
J. Ind. Microbiol. Biotechnol.
35
713-720
2008
Alcaligenes faecalis, Alcaligenes faecalis MTCC 126
Ankati, H.; Zhu, D.; Yang, Y.; Biehl, E.R.; Hua, L.
Asymmetric synthesis of both antipodes of beta-hydroxy nitriles and beta-hydroxy carboxylic acids via enzymatic reduction or sequential reduction/hydrolysis
J. Org. Chem.
74
1658-1662
2009
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
Gupta, V.; Gaind, S.; Verma, P.; Sood, N.; Srivastava, A.
Purification and characterization of intracellular nitrilases from Rhodococcus sp. - potential role of periplasmic nitrilase
Afr. J. Microbiol. Res.
4
1148-1153
2010
Rhodococcus rhodochrous, Rhodococcus fascians, Rhodococcus fascians MTCC-1531, Rhodococcus rhodochrous MTCC-291
-
Malandra, A.; Cantarella, M.; Kaplan, O.; Vejvoda, V.; Uhnakova, B.; Stepankova, B.; Kubac, D.; Martinkova, L.
Continuous hydrolysis of 4-cyanopyridine by nitrilases from Fusarium solani O1 and Aspergillus niger K10
Appl. Microbiol. Biotechnol.
85
277-284
2009
Aspergillus niger, Fusarium solani, Fusarium solani O1, Aspergillus niger K10
Williamson, D.S.; Dent, K.C.; Weber, B.W.; Varsani, A.; Frederick, J.; Thuku, R.N.; Cameron, R.A.; van Heerden, J.H.; Cowan, D.A.; Sewell, B.T.
Structural and biochemical characterization of a nitrilase from the thermophilic bacterium, Geobacillus pallidus RAPc8
Appl. Microbiol. Biotechnol.
88
143-153
2010
Aeribacillus pallidus (Q0PIV8), Aeribacillus pallidus, Aeribacillus pallidus RAPc8 (NRRL: B-59396) (Q0PIV8)
Bayer, S.; Birkemeyer, C.; Ballschmiter, M.
A nitrilase from a metagenomic library acts regioselectively on aliphatic dinitriles
Appl. Microbiol. Biotechnol.
89
91-98
2011
uncultured bacterium (E1A0Z9)
Vesela, A.B.; Franc, M.; Pelantova, H.; Kubac, D.; Vejvoda, V.; Sulc, M.; Bhalla, T.C.; Mackova, M.; Lovecka, P.; Jan?, P.; Demnerova, K.; Martinkova, L.
Hydrolysis of benzonitrile herbicides by soil actinobacteria and metabolite toxicity
Biodegradation
21
761-770
2010
Nocardia globerula, Rhodococcus rhodochrous, Rhodococcus sp., Rhodococcus sp. NDB 1165, Nocardia globerula NHB-2, Rhodococcus rhodochrous PA-34
Kumar, S.; Mohan, U.; Kamble, A.L.; Pawar, S.; Banerjee, U.C.
Cross-linked enzyme aggregates of recombinant Pseudomonas putida nitrilase for enantioselective nitrile hydrolysis
Biores. Technol.
101
6856-6858
2010
Pseudomonas putida, Pseudomonas putida MTCC 5110
Schreiner, U.; Hecher, B.; Obrowsky, S.; Waich, K.; Klempier, N.; Steinkellner, G.; Gruber, K.; Rozzell, J.; Glieder, A.; Winkler, M.
Directed evolution of Alcaligenes faecalis nitrilase
Enzyme Microb. Technol.
47
140-146
2010
Alcaligenes faecalis (P20960), Alcaligenes faecalis JM3 (P20960)
-
Yeom, S.J.; Lee, J.K.; Oh, D.K.
A positively charged amino acid at position 129 in nitrilase from Rhodococcus rhodochrous ATCC 33278 is an essential residue for the activity with meta-substituted benzonitriles
FEBS Lett.
584
106-110
2010
Rhodococcus rhodochrous, Rhodococcus rhodochrous ATCC 33278
Agerbirk, N.; Olsen, C.E.; Poulsen, E.; Jacobsen, N.; Hansen, P.R.
Complex metabolism of aromatic glucosinolates in Pieris rapae caterpillars involving nitrile formation, hydroxylation, demethylation, sulfation, and host plant dependent carboxylic acid formation
Insect Biochem. Mol. Biol.
40
126-137
2010
Arabis hirsuta
Thuku, R.; Brady, D.; Benedik, M.; Sewell, B.
Microbial nitrilases: Versatile, spiral forming, industrial enzymes
J. Appl. Microbiol.
106
703-727
2009
Arthrobacter sp., Bradyrhizobium japonicum, Brassica napus, Fusarium solani, Rhodococcus rhodochrous, Rhodococcus sp., Fusarium oxysporum f. sp. melonis, Aspergillus niger (A9QXE0), Arabidopsis thaliana (P32961), Aeribacillus pallidus (Q0PIV8), Bradyrhizobium japonicum USDA 110, Rhodococcus sp. NCIMB 11216, Fusarium solani IMI 196840, Fusarium solani O1, Aeribacillus pallidus Dac521 (Q0PIV8), Rhodococcus rhodochrous ATCC 39484, Aspergillus niger K10 (A9QXE0), Rhodococcus sp. NCIMB 11215, Rhodococcus rhodochrous PA-34
Winkler, M.; Kaplan, O.; Vejvoda, V.; Klempier, N.; Martinkova, L.
Biocatalytic application of nitrilases from Fusarium solani O1 and Aspergillus niger K10
J. Mol. Catal. B
59
243-247
2009
Aspergillus niger, Fusarium solani, Fusarium solani O1, Aspergillus niger K10
-
Vejvoda, V.; Kubac, D.; Davidova, A.; Kaplan, O.; Sulc, M.; Sveda, O.; Chaloupkova, R.; Martinkova, L.
Purification and characterization of nitrilase from Fusarium solani IMI196840
Process Biochem.
45
1115-1120
2010
Fusarium solani, Fusarium solani IMI196840, Fusarium solani O1
-
Zhang, Z.; Xu, J.; He, Y.; Ouyang, L.; Liu, Y.; Imanaka, T.
Efficient production of (R)-(-)-mandelic acid with highly substrate/product tolerant and enantioselective nitrilase of recombinant Alcaligenes sp.
Process Biochem.
45
887-891
2010
Alcaligenes sp. (C5IIS9), Alcaligenes sp. ECU0401 (C5IIS9)
-
Yang, C.; Wang, X.; Wei, D.
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Cereibacter sphaeroides, Cereibacter sphaeroides LHS-305
Baum, S.; Williamson, D.S.; Sewell, T.; Stolz, A.
Conversion of sterically demanding alpha,alpha-disubstituted phenylacetonitriles by the arylacetonitrilase from Pseudomonas fluorescens EBC191
Appl. Environ. Microbiol.
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Alcaligenes faecalis, Synechocystis sp., Rhodococcus sp., Pseudomonas fluorescens (Q5EG61), Pseudomonas fluorescens, Pseudomonas fluorescens EBC191 (Q5EG61), Pseudomonas fluorescens EBC191, Alcaligenes faecalis ATCC 8750
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Purification and characterization of heterologously expressed nitrilases from filamentous fungi
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Talaromyces marneffei (B6Q5I3), Fusarium verticillioides (Q19A54), Fusarium verticillioides, Talaromyces marneffei ATCC 18224 (B6Q5I3), Talaromyces marneffei ATCC 18224
Zhang, Z.J.; Xu, J.H.; He, Y.C.; Ouyang, L.M.; Liu, Y.Y.
Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(-)-mandelic acid production
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Alcaligenes sp. (C5IIS9), Alcaligenes sp. ECU0401 (C5IIS9)
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Optimization of nitrilase production from Alcaligenes faecalis MTCC 10757 (IICT-A3): effect of inducers on substrate specificity
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Alcaligenes faecalis, Alcaligenes faecalis MTCC 10757
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Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
BMC Biotechnol.
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Aspergillus niger (A9QXE0), Aspergillus niger K10 (A9QXE0), Aspergillus niger K10
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Biosynthesis of iminodiacetic acid from iminodiacetonitrile by immobilized recombinant Escherichia coli harboring nitrilase
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Acidovorax facilis (Q1W2L4), Acidovorax facilis
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Expression control of nitrile hydratase and amidase genes in Rhodococcus erythropolis and substrate specificities of the enzymes
Antonie van Leeuwenhoek
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Rhodococcus erythropolis (B1GY01), Rhodococcus erythropolis A4 (B1GY01)
Duca, D.; Rose, D.R.; Glick, B.R.
Characterization of a nitrilase and a nitrile hydratase from Pseudomonas sp. UW4 that converts indole-3-acetonitrile to produce indole-3-acetic acid
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Pseudomonas sp. (K9NKH3), Pseudomonas sp.
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Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization
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Fusarium proliferatum, Fusarium proliferatum CA3-1
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Characterization of a nitrilase from Arthrobacter aurescens CYC705 for synthesis of iminodiacetic acid
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Paenarthrobacter aurescens (S5M6X9), Paenarthrobacter aurescens, Paenarthrobacter aurescens CYC705 (S5M6X9)
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Characterization and functional cloning of an aromatic nitrilase from Pseudomonas putida CGMCC3830 with high conversion efficiency toward cyanopyridine
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Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties
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Arabidopsis thaliana (P32961), Arabidopsis thaliana
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Classifying nitrilases as aliphatic and aromatic using machine learning technique
3 Biotech
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Aspergillus luchuensis (G7XTA8), Aspergillus luchuensis IFO 4308 (G7XTA8)
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Characterization of a new nitrilase from Hoeflea phototrophica DFL-43 for a two-step one-pot synthesis of (S)-?-amino acids
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Hoeflea phototrophica, Hoeflea phototrophica DFL-43
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Improving catalytic performance of an arylacetonitrilase by semirational engineering
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uncultured organism (Q6RWE0)
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Pseudomonas putida
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Paenarthrobacter aurescens, Paenarthrobacter aurescens CYC705
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Pannonibacter carbonis, Pannonibacter carbonis Q4.6
Sonbol, S.A.; Ferreira, A.J.; Siam, R.
Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance
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uncultured Burkholderiaceae bacterium (A0A140D8I8), Cereibacter sphaeroides (G5DDB2), Cereibacter sphaeroides LHS-305 (G5DDB2)
Yu, S.; Yao, P.; Li, J.; Feng, J.; Wu, Q.; Zhu, D.
Improving the catalytic efficiency and stereoselectivity of a nitrilase from Synechocystis sp. PCC6803 by semi-rational engineering en route to chiral gamma-amino acids
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Synechocystis sp. PCC 6803 (Q55949)
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Capsella rubella (R0GJB9), Capsella rubella (R0H4W3), Capsella rubella
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Engineering the residues on "A" surface and C-terminal region to improve thermostability of nitrilase
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Acidovorax facilis (A0A0U1TDG4), Acidovorax facilis ZJB09122 (A0A0U1TDG4)
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Cloning, overexpression, and characterization of a thermostable nitrilase from an antarctic Pyrococcus sp
Extremophiles
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Pyrococcus sp. MC-FB
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A new thermophilic nitrilase from an antarctic hyperthermophilic microorganism
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Pyrococcus sp. M24D13
Lehmann, T.; Janowitz, T.; Sanchez-Parra, B.; Alonso, M.P.; Trompetter, I.; Piotrowski, M.; Pollmann, S.
Arabidopsis nitrilase 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings
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Arabidopsis thaliana (P32961), Arabidopsis thaliana
Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.
Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles
Funct. Integr. Genomics
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Fusarium proliferatum (A0A0E3D8K7), Fusarium proliferatum, Fusarium proliferatum AUF-2 (A0A0E3D8K7), Fusarium proliferatum AUF-2
Liu, Z.Q.; Lu, M.M.; Zhang, X.H.; Cheng, F.; Xu, J.M.; Xue, Y.P.; Jin, L.Q.; Wang, Y.S.; Zheng, Y.G.
Significant improvement of the nitrilase activity by semi-rational protein engineering and its application in the production of iminodiacetic acid
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Acidovorax facilis (Q1W2L4), Acidovorax facilis
Xu, Z.; Huang, J.W.; Xia, C.J.; Zou, S.P.; Xue, Y.P.; Zheng, Y.G.
Enhanced catalytic stability and reusability of nitrilase encapsulated in ethyleneamine-mediated biosilica for regioselective hydrolysis of 1-cyanocycloalkaneacetonitrile
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Acidovorax facilis (Q1W2L4), Acidovorax facilis
Badoei-Dalfard, A.; Ramezani-Pour, N.; Karami, Z.
Production and characterization of a nitrilase from Pseudomonas aeruginosa RZ44 and its potential for nitrile biotransformation
Iran. J. Biotechnol.
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Pseudomonas aeruginosa, Pseudomonas aeruginosa RZ44
Cai, W.; Su, E.; Zhu, S.; Ren, Y.; Ren, Y.; Wei, D.
Characterization of a nitrilase from Arthrobacter aurescens cyc705 for synthesis of iminodiacetic acid
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Paenarthrobacter aurescens (S5M6X9)
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Chen, H.; Zhang, T.; Sun, T.; Ni, Z.; Le, Y.; Tian, R.; Chen, Z.; Zhang, C.
Clostridium thermocellum nitrilase expression and surface display on Bacillus subtilis spores
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Acetivibrio thermocellus (A3DHT2), Acetivibrio thermocellus
Ricano-Rodrguez, J.; Ramrez-Lepe, M.
Purification and characterization of a cyanide-degrading nitrilase from Trichoderma harzianum VSL291
Turk. J. Biol.
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Trichoderma harzianum, Trichoderma harzianum VSL291
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