We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Synonyms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate hydrolase
-
-
Formyl-FH(4) hydrolase
-
-
-
-
formyltetrahydrofolate hydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate
-
-
-
-
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate
mechanism
-
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate
dehydrogenase reaction requires full length enzyme, hydrolase reaction occurs in amino-terminal domain of enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MetaCyc
folate transformations I, formaldehyde oxidation VII (THF pathway), purine nucleobases degradation I (anaerobic), purine nucleobases degradation II (anaerobic)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate amidohydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
-
Substrates: -
?
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
Substrates: -
?
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
-
Substrates: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
-
Substrates: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NADP+
-
required for dehydrogenase reaction
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.007 - 0.049
10-formyltetrahydrofolate
0.007
10-formyltetrahydrofolate
-
30°C, pH 7.8, wild type
0.0083
10-formyltetrahydrofolate
-
30°C, pH 7.8, H125A mutant
0.009
10-formyltetrahydrofolate
-
30°C, pH 7.8, H113A mutant
0.0095
10-formyltetrahydrofolate
-
30°C, pH 7.8, H100A mutant
0.049
10-formyltetrahydrofolate
2.0 mM methionine added
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cow
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
no activity in Columba sp.
pigeon
-
-
brenda
-
-
-
brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
31800
calculated from the sequence of the cloned gene
32000
6 * 32000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hexamer
6 * 32000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolase domain, amino-terminal domain of enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
H100A
-
soluble, shows enzymic activity
H106A
-
insoluble, no enzymatic activity
H106E
-
insoluble, no enzymatic activity
H106K
-
insoluble, no enzymatic activity
H106N
-
insoluble, no enzymatic activity
H106Q
-
insoluble, no enzymatic activity
H106R
-
insoluble, no enzymatic activity
H113A
-
insoluble, shows enzymic activity
H125A
-
soluble, shows enzymic activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
purU gene cloned into overexpression vector pT7-7, pT7-PU1 tranformed into Escherichia coli BL21(DE3)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Huennekens, F.M.
Enzymatic deacylation of N10-formyltetrahydrofolic acid
Fed. Proc.
16
199
1957
Bos taurus, no activity in Columba sp.
-
brenda
Nagy, P.L.; Marolewski, A.; Benkovic, S.J.; Zalkin, H.
Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli
J. Bacteriol.
3
1292-1298
1995
Escherichia coli (P37051), Escherichia coli
brenda
Krupenko, S.A.; Vlasov, A.P.; Wagner, C.
On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis
J. Biol. Chem.
276
24030-24037
2001
Rattus norvegicus
brenda
Chumanevich, A.A.; Krupenko, S.A.; Davies, C.
The crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase: mechanism of hydrolysis and its interplay with the dehydrogenase domain
J. Biol. Chem.
279
14355-14364
2004
Rattus norvegicus
brenda
Suzuki, H.; Tamamura, R.; Yajima, S.; Kanno, M.; Suguro, M.
Corynebacterium sp. U-96 contains a cluster of genes of enzymes for the catabolism of sarcosine to pyruvate
Biosci. Biotechnol. Biochem.
69
952-956
2005
Corynebacterium sp.
brenda
html completed
show
top
