in the presence of heparin non-inactivating cleavage in aminoterminal region at Glu37-Gln38 followed by inactivating cleavages in carboxyterminal region at Ala375-Ser376 and Ala378-Val379, in the absence of heparin inactivating cleavage in carboxyterminal region at Ser383-Ala384
first occuring fragment in the presence of heparin is a still active antithrombin of MW 56000, followed by inactive fragments of MW 50000 and MW 5000, in the absence of heparin only inactive fragments of MW 56000 and MW 5000 occur
first occuring fragment in the presence of heparin is a still active antithrombin of MW 56000, followed by inactive fragments of MW 50000 and MW 5000, in the absence of heparin only inactive fragments of MW 56000 and MW 5000 occur
snake venom zincendopeptidase, hydrolyzes basement membrane proteins involved in the adhesion among capillary endothelial cells, inducing extensive haemorrhages in the bitten preys
snake venom zincendopeptidase, hydrolyzes basement membrane proteins involved in the adhesion among capillary endothelial cells, inducing extensive haemorrhages in the bitten preys
zinc-binding motif, almost tetrahedrally co-ordinated by His142, His146 and His152 and a water molecule anchored to an intermediate Glu residue, Glu143
2 mM Zn2+ protects at 2 mM 2-mercaptoethanol, 2 mM Zn2+ partially protects at 10-50 mM 2-mercaptoethanol, 2 mM Ca2+ partially protects at 2-10 mM 2-mercaptoethanol, no protection at 50 mM 2-mercaptoethanol
2 mM Zn2+ protects at 0.5-1 mM EDTA, no protection at 2 mM EDTA, 2 mM Ca2+ partially protects at 0.5 mM EDTA, weakly at 1 mM EDTA, no protection at 2 mM EDTA; hide powder azure or alpha1-proteinase inhibitor as substrates
Gene Electrotransfer of Plasmid Antiangiogenic Metargidin Peptide (AMEP) in Disseminated Melanoma: Safety and Efficacy Results of a Phase I First-in-Man Study.
Characterization of growth factor-induced serine phosphorylation of tumor necrosis factor-alpha converting enzyme and of an alternatively translated polypeptide.
P2Y2 nucleotide receptors mediate metalloprotease-dependent phosphorylation of epidermal growth factor receptor and ErbB3 in human salivary gland cells.
shares a similar overall topology with astacin, with the collagenase catalytic domains and the Pseudomonas aeruginosa alkaline proteinase and exhibits a virtual identical zinc environment with these other metzincins
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complex with phosphate inhibitor, crystals obtained by sitting drop vapour diffusion technique, space group P3(2)12, cell parameters a : b : 73.5 A, c : 96.9 A
recombinant disintegrin domain of enzyme, shows inhibitory activities on endothelial cell proliferation and adhesion on the integrin substrates fibronectin, vitronectin, and fibrinogen.The domain totally abrogates endothelial cell migration and blocks most capillary formation in a three-dimensional fibrin gel. Athymic mice bearing electrotransferred domain exhibit strong inhibition of tumor angiogenesis
recombinant disintegrin domain of enzyme, shows inhibitory activities on endothelial cell proliferation and adhesion on the integrin substrates fibronectin, vitronectin, and fibrinogen.The domain totally abrogates endothelial cell migration and blocks most capillary formation in a three-dimensional fibrin gel. Athymic mice bearing electrotransferred domain exhibit strong inhibition of tumor angiogenesis
Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc-binding environments (HEXXHXXGXXH and Met-turn) and topologies and should be grouped into a common family, the metzincins
Identification of the cleavage sites resulting from enzymatic inactivation of human antithrombin III by Crotalus adamanteus proteinase II in the the presence and absence of heparin
Refined 2.0 A X-ray crystal structure of the snake venom zinc-endopeptidase adamalysin II. Primary and tertiary structure determination, refinement, molecular structure and comparison with astacin, collagenase and thermolysin