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IUBMB CommentsDoes not hydrolyse disaccharides or oligosaccharides containing linear 1,2-alpha-glucosidic linkages.
The expected taxonomic range for this enzyme is: Flavobacterium sp.
Synonyms
dextranhydrolase,
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dextran 1,2-alpha glucosidase
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dextran alpha-(1-->2)-debranching enzyme
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dextran alpha-1,2 debranching enzyme
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glucosidase, dextran 1,2-alpha
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Glcalpha(1-6)Glcalpha(1-6)[Glcalpha(1-2)]Glcalpha(1-6)Glcalpha(1-6)Glcalpha(1-6)Glcalpha(1-6) + H2O = Glcalpha(1-6)Glcalpha(1-6)Glcalpha(1-6)Glcalpha(1-6)Glcalpha(1-6)Glcalpha(1-6) + alpha-D-glucopyranose
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hydrolysis of O-glycosyl bond
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(1->2)-alpha-D-glucosyl-branched-dextran 2-glucohydrolase
Does not hydrolyse disaccharides or oligosaccharides containing linear 1,2-alpha-glucosidic linkages.
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2(3),2(4)-di-O-alpha-glucosyl-isomaltotetraose + H2O
isomaltotetraose + 2 alpha-D-glucose
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2(3)-O-alpha-D-glucosyl-isomaltotetraose + H2O
isomaltotetraose + alpha-D-glucose
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2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O
isomaltotriose + alpha-D-glucose
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B-1298 soluble dextran + H2O
D-glucose + ?
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incubation at 40°C for 24 h, specifically splitz alpha-1,2-glucosidic linkages
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B-1299 soluble dextran + H2O
D-glucose + ?
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incubation at 40°C for 24 h
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B-1397 soluble dextran + H2O
D-glucose + ?
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incubation at 40°C for 24 h
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dextran + H2O
alpha-D-glucose
mutan IG-1 + H2O
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major linkages: alpha-1,3-, alpha-1,6-, 18% as effective as soluble dextran for induction of enzyme
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additional information
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dextran + H2O
alpha-D-glucose
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B-1299, soluble, major linkages: alpha-1,6-, alpha-1,3-, alpha-1,2-, most effective substrate for induction of enzyme
hydrolysis of 1,2-alpha-D-glucosidic linkages at the branched points, producing free D-glucose
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dextran + H2O
alpha-D-glucose
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clinical dextran, major linkages: alpha-1,6-, alpha-1,3-, 55% as effective as soluble dextran for induction of enzyme
hydrolysis of 1,2-alpha-D-glucosidic linkages at the branched points, producing free D-glucose
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additional information
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effect of different carbon sources on enzyme activity, no activity with alpha-1,3-glucan, starch, glucose or sucrose
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additional information
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hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran
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additional information
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hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran
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Al3+
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1.33 mM, 0.9% relative activity
Cu2+
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1.33 mM, 1.7% relative activity
D-glucono-1,5-lactone
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strong inhibitor, 34.2% relative activity
Fe3+
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1.33 mM, 2.6% relative activity
Hg2+
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1.33 mM, 1.2% relative activity
iodoacetic acid
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still 60.1% relative activity
Sodium dodecyl sulfate
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strong inhibitor, 37.9% relative activity
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additional information
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effect of different carbon sources on enzyme activity, inducibility of enzyme
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0.21
B-1298 soluble dextran
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mg/ml
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0.27
B-1299 soluble dextran
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mg/ml
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0.91
B-1397 soluble dextran
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mg/ml
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additional information
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5.8 micro-g glucose/ml, substrate B-1298 soluble dextran
additional information
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5.8 micro-g glucose/ml, substrate B-1299 soluble dextran
additional information
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5.8 micro-g glucose/ml, substrate B-1397 soluble dextran
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brenda
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125000
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gel electrophoresis
125000
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x * 125000, SDS-PAGE
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6.5 - 9
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incubation at 4°C for 24 h
697134
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40
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up to 40°C on heating for 10 min
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Mitsuishi,Y.; Kobayashi, M.; Matsuda, K.
Dextran alpha-1,2 debranching enzyme from Flavobacterium sp. M-73: Its production and purification
Agric. Biol. Chem.
43
2283-2290
1979
Flavobacterium sp.
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brenda
Mitsuishi , M.Y.; Kobayashi , K.M.; Matsuda , M.K.
Dextran alpha-(1 yields 2)-debranching enzyme from Flavobacterium Sp. M-73. Properties and mode of action
Carbohydr. Res.
83
303-313
1980
Flavobacterium sp.
brenda
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3.2.1.115 branched-dextran exo-1,2-alpha-glucosidase
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