Information on EC 3.1.3.104 - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase

for references in articles please use BRENDA:EC3.1.3.104
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.3.104
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RECOMMENDED NAME
GeneOntology No.
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O = 5-amino-6-(D-ribitylamino)uracil + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
flavin biosynthesis I (bacteria and plants)
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flavin biosynthesis II (archaea)
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flavin biosynthesis III (fungi)
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toxoflavin biosynthesis
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Riboflavin metabolism
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphohydrolase
Requires Mg2+. The enzyme, which is found in plants and bacteria, is part of a pathway for riboflavin biosynthesis. Most forms of the enzyme has a broad substrate specificity [1,3].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O
5-amino-6-(D-ribitylamino)uracil + phosphate
show the reaction diagram
6-phospho-D-gluconate + H2O
D-gluconate + phosphate
show the reaction diagram
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
D-ribitol 1-phosphate + H2O
D-ribitol + phosphate
show the reaction diagram
D-ribitol 5-phosphate + H2O
D-ribitol + phosphate
show the reaction diagram
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
FMN + H2O
riboflavin + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O
5-amino-6-(D-ribitylamino)uracil + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
about 50% of the activity with Mg2+
Mg2+
Mg2+ affords maximum catalytic activity over 1-5 mM, half-maximal velocity at 0.2 mM
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.054 - 0.128
5-amino-6-(5-phospho-D-ribitylamino)uracil
1.03
D-ribitol 1-phosphate
pH 25°C, pH 7.5
1.08
D-ribitol 5-phosphate
pH 25°C, pH 7.5
0.0142 - 0.135
FMN
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 2.8
5-amino-6-(5-phospho-D-ribitylamino)uracil
1.8
D-ribitol 1-phosphate
pH 25°C, pH 7.5
1.5
D-ribitol 5-phosphate
pH 25°C, pH 7.5
0.59
FMN
pH 8.0, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16 - 22.1
5-amino-6-(5-phospho-D-ribitylamino)uracil
1.7
D-ribitol 1-phosphate
pH 25°C, pH 7.5
1.4
D-ribitol 5-phosphate
pH 25°C, pH 7.5
41.5
FMN
pH 8.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0041
substrate D-glucose 6-phosphate, pH not specified in the publication, temperature not specified in the publication
0.0061
substrate ribose 5-phosphate, pH not specified in the publication, temperature not specified in the publication
0.0063
substrate 6-phospho-D-gluconate, pH not specified in the publication, temperature not specified in the publication
0.69
pH not specified in the publication, temperature not specified in the publication
1.7
pH not specified in the publication, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46300
gel filtration
72100
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 25000, calculated; 2 * 36000, calculated
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli