Information on EC 3.1.2.B4 - (3S)-malyl-CoA thioesterase

for references in articles please use BRENDA:EC3.1.2.B4
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The expected taxonomic range for this enzyme is: Rhodobacter sphaeroides

EC NUMBER
COMMENTARY hide
3.1.2.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
(3S)-malyl-CoA thioesterase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3S)-malyl-CoA + H2O = (S)-malate + CoA
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(3S)-malyl-CoA hydrolase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Rhodobacter sphaeroides ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S)-malyl-CoA + H2O
(S)-malate + CoA
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
(3S)-malyl-CoA
pH 7.5, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
110
(3S)-malyl-CoA
pH 7.5, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1200
(3S)-malyl-CoA
pH 7.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33400
homodimer or homotrimer, x * 33400 (calculated)
80000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged version expressed in Escherichia coli BL21(DE3)