Information on EC 2.7.8.42 - Kdo2-lipid A phosphoethanolamine 7''-transferase

for references in articles please use BRENDA:EC2.7.8.42
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.8.42
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RECOMMENDED NAME
GeneOntology No.
Kdo2-lipid A phosphoethanolamine 7''-transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A = diacylglycerol + 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A
show the reaction diagram
(1)
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diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA = diacylglycerol + 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA
show the reaction diagram
(2)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lipopolysaccharide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
diacylphosphatidylethanolamine:alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid-A 7''-phosphoethanolaminetransferase
The enzyme has been characterized from the bacterium Escherichia coli. It is activated by Ca2+ ions and is is silenced by the sRNA MgrR.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme has a dual role in modifying the flagellar rod protein, FlgG, and the lipid A domain of Campylobacter jejuni lipooligosaccharide with a phosphoethanolamine residue
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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Hfq-dependent small RNA MgrR negatively regulates expression of EptB. Envelope stress response protein Sigma E has positively regulates EptB. The effects of Sigma E and deletion of MgrR on levels of EptB mRNA are independent, and the same 5' end is found in both cases. Sigma E acts directly at the level of transcription initiation for EptB, from the same start point as Sigma 70. When Sigma E is active, synthesis of EptB transcript may outstrip MgrR-dependent degradation. A second sRNA, ArcZ, also directly and negatively regulates EptB
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A
diacylglycerol + 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A
show the reaction diagram
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in the presence of Ca2+, enzyme transfers the phosphatidylethanolamine group. Stoichiometric amounts of diacylglycerol are generated during the transfer to Kdo2-lipid A
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?
diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA
diacylglycerol + 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA
show the reaction diagram
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the phosphoethanolamine substituent is located on the outer Kdo moiety
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?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Hfq-dependent small RNA MgrR negatively regulates expression of EptB. Envelope stress response protein Sigma E has positively regulates EptB. The effects of Sigma E and deletion of MgrR on levels of EptB mRNA are independent, and the same 5? end is found in both cases. Sigma E acts directly at the level of transcription initiation for EptB, from the same start point as Sigma 70. When Sigma E is active, synthesis of EptB transcript may outstrip MgrR-dependent degradation. A second sRNA, ArcZ, also directly and negatively regulates EptB
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membranes from Escherichia coli grown on 5-50 mM CaCl2 contain a phosphoethanolamine transferase that uses the precursor Kdo2-[4*-32P]lipid IVA as an acceptor. Transferase is not present in membranes of Escherichia coli grown with 5 mM MgCl2, BaCl2, or ZnCl2
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