We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)- (phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
cpep phosphonomutase, carboxyphosphonoenolpyruvate phosphonomutase, carboxyphosphoenolpyruvate mutase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
carboxyphosphoenolpyruvate mutase
-
-
carboxyphosphonoenolpyruvate phosphonomutase
-
-
-
-
CPEP phosphonomutase
-
-
-
-
CPEP mutase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-carboxyvinyl carboxyphosphonate = 3-(hydroxyphosphinoyl)pyruvate + CO2
catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)-(phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
substituted phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-carboxyvinyl carboxyphosphonate phosphorylmutase (decarboxylating)
Catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)- (phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-carboxyvinyl carboxyphosphonate
3-(hydrohydroxyphosphoryl)pyruvate + CO2
-
-
-
?
carboxyphosphinopyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphoenolpyruvate
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
carboxyphosphoenolpyruvate
?
-
-
-
?
carboxyphosphoenolpyruvate
?
-
-
-
-
?
carboxyphosphoenolpyruvate
?
-
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
first natural phosphinate
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
first natural phosphinate
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-carboxyvinyl carboxyphosphonate
3-(hydrohydroxyphosphoryl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
first natural phosphinate
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
first natural phosphinate
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect
Mg2+
-
increases activity, 1-10 mM MgSO4, relative activity 250%
Mg2+
-
reaction shows metal dependence
Mn2+
-
increases activity, about 10times more active compared with the activity in presence of Mg2+
Mn2+
-
1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
-
enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4
Phosphonopyruvate
-
5 mM, 50% inhibition
additional information
-
phosphoenolpyruvate shows almost no effect
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0022
carboxyphosphinopyruvate
-
pH 6.5, 30°C
0.25 - 0.27
carboxyphosphoenolpyruvate
0.25
carboxyphosphoenolpyruvate
-
pH 6.5, 27°C
0.27
carboxyphosphoenolpyruvate
-
-
0.27
carboxyphosphoenolpyruvate
-
pH 6.5, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00076
carboxyphosphinopyruvate
-
pH 7.5, 30°C
0.02
carboxyphosphoenolpyruvate
0.02
carboxyphosphoenolpyruvate
-
-
0.02
carboxyphosphoenolpyruvate
-
pH 7.5, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
specific activity 37.6 U/mg x 1000
additional information
-
specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.1
calculated from amino acid sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
no activity in Streptomyces hygroscopicus
-
-
-
brenda
-
UniProt
brenda
-
UniProt
brenda
SF1293
-
-
brenda
-
-
-
brenda
ATCC 21705
-
-
brenda
HP782 and mutant NP71
-
-
brenda
HP782, HP5-29 and SF1293 mutant NP71
-
-
brenda
SF1293
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
the enzyme plays a role that is related to the tillering regulation in rice
physiological function
between a heat-tolerant strain (HTS) and heat-sensitive coisogenic rice strain (HSS), following short-term extreme high nighttime temperature stress at the first stage of seed ripening, 56 genes are differentially expressed. Unique SNPs located on the genes peroxidase precursor, glutathione S-transferase GSTU6, glycosyl hydrolases, carboxyvinylcarboxyphosphonate phosphorylmutase, and prolamin precursor PROLM3 are present in HTS but absent from HSS and show slight alterations in gene expression between HTS and HSS. Carboxyvinyl-carboxyphosphonate phosphorylmutase is downregulated 1.37fold in HTS but upregulated 7.50fold in HSS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
32000
-
2 * 32000, homodimer, SDS-PAGE
32700
-
calculated from amino acid composition
32800
-
calculated from amino acid composition
38000
x * 38000, calculated from amino acid sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 38000, calculated from amino acid sequence
dimer
-
2 * 32000, homodimer, SDS-PAGE
dimer
-
2 * 32000, homodimer, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.5 - 8
-
stable in this range
643769, 643773
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month
-
4°C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cloned and expressd at high levels in Escherichia coli
-
gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3)
-
mutant NP71 constructed by gene replacement technique
-
nucleotide sequence of gene isolated and cloned and expressed in Streptomyces lividans 66 using expression vector pIJ680
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
the mRNA level is down-regulated in the high-tillering d10 dwarf plants
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
agriculture
-
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
agriculture
-
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Hidaka, T.; Hara, O.; Imai, S.; Anzai, H.; Murakami, T.; Nakaoka, K.; Seto, H.
Biochemical mechanism of C-P bond formation of bialaphos: use of gene manipulation for the analysis of the C-P bond formation step
Agric. Biol. Chem.
54
2121-2125
1990
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
brenda
Hidaka, T.; Imai, S.; Hara, O.; Anzai, H.; Murakami, T.; Nagaoka, K.; Seto, H.
Carboxyphosphoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation
J. Bacteriol.
172
3066-3072
1990
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
brenda
Hidaka, T.; Seto, H.
Comparison of two C-P bond-forming enzymes involved in the biosynthesis of bialaphos
Agric. Biol. Chem.
54
2467-2468
1990
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
-
brenda
Freeman, S.; Pollack, S.J.; Knowles, J.R.
Synthesis of the unusual metabolite carboxyphosphoenolpyruvate. Cloning and expression of carboxyphosphonoenolpyruvate mutase
J. Am. Chem. Soc.
114
377-378
1992
Streptomyces hygroscopicus
-
brenda
Hidaka, T.; Hidaka, M.; Uozumi, T.; Seto, H.
Nucleotide sequence of a carboxyphosphoenolpyruvate phosphonomutase gene isolated from a bialaphos-producing organism, Streptomyces hygroscopicus, and its expression in Streptomyces lividans
Mol. Gen. Genet.
233
476-478
1992
Streptomyces hygroscopicus
brenda
Pollack, S.J.; Freeman, S.; Pompliano, D.L.; Knowles, F.R.
Cloning, overexpression and mechanistic studies of carboxyphosphoenolpyruvate mutase from Streptomyces hygroscopicus
Eur. J. Biochem.
209
735-743
1992
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus
brenda
Chen, F.; Jiang, L.; Zheng, J.; Huang, R.; Wang, H.; Hong, Z.; Huang, Y.
Identification of a co-segregative protein associated with the tillering trait in rice (Oryza sativa L.)
Plant OMICS
6
36-45
2013
Oryza sativa (Q2QWN6)
-
brenda
Zhou, H.; Zhang, H.; Wang, Z.; He, C.; You, L.; Fu, D.; Song, J.; Huang, Y.; Liao, J.
Discovery of unique single nucleotide polymorphisms in rice in response to high nighttime temperature stress using a hybrid sequencing strategy
Environ. Exp. Bot.
162
48-57
2019
Oryza sativa Japonica Group (Q2QWN6)
-
brenda
Select items on the left to see more content.
html completed