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IUBMB Comments The enzyme, isolated from the bacterium Streptomyces wedmorensis, is involved in fosfomycin biosynthesis. The enzyme also is active as EC 5.4.2.9 phosphoenolpyruvate mutase.
The expected taxonomic range for this enzyme is: Streptomyces wedmorensis
Synonyms
Fom1, Fom1CyTase,
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Fom1
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Fom1
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Fom1 contains a cytidylyltransferase domain at its N-terminus
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2-hydroxyethylphosphonate + CTP = cytidine 5'-{[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate} + diphosphate
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CTP:2-hydroxyethylphosphonate cytidylyltransferase
The enzyme, isolated from the bacterium Streptomyces wedmorensis, is involved in fosfomycin biosynthesis. The enzyme also is active as EC 5.4.2.9 phosphoenolpyruvate mutase.
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2-hydroxyethylphosphonate + CTP
cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate] + diphosphate
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2-hydroxyethylphosphonate + CTP
cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate] + diphosphate
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Co2+
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required for activity. 5 mM used in assay conditions
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native enzyme in complex with cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate], hanging drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.5) and 4.5 M NaCl
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Ni2+-affinity column chromatography
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and B834 (DE3) cells
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Cho, S.H.; Kim, S.Y.; Tomita, T.; Shiraishi, T.; Park, J.S.; Sato, S.; Kudo, F.; Eguchi, T.; Funa, N.; Nishiyama, M.; Kuzuyama, T.
Fosfomycin biosynthesis via transient cytidylylation of 2-hydroxyethylphosphonate by the bifunctional Fom1 enzyme
ACS Chem. Biol.
12
2209-2215
2017
Streptomyces wedmorensis
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