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7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
-
-
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
-
-
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
-
-
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
-
-
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction mechanism of RaSEA, overview
-
-
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine = 5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA + S-adenosyl-L-methionine
5'-deoxyadenosine + archaeosine15 in tRNA + 2 H+ + L-1-piperideine-6-carboxylate + L-methionine
reaction of subunit beta
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
evolution
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
evolution
-
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
-
evolution
-
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
-
evolution
-
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
-
evolution
-
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
-
evolution
-
the hyperthermophilic euryarchaeon Thermococcus kodakarensis lacks an arcTGT orthologue
-
malfunction
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
malfunction
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
malfunction
-
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
-
malfunction
-
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
-
malfunction
-
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
-
malfunction
-
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
-
malfunction
-
in a TK1135 disruption strain (DELTATK1135), the G+ peak disappears from the tRNA fraction and a small q0kN peak is observed
-
metabolism
archaeosine biosynthesis pathway, overview
metabolism
archaeosine biosynthesis pathway, overview
metabolism
-
archaeosine biosynthesis pathway, overview
-
metabolism
-
archaeosine biosynthesis pathway, overview
-
metabolism
-
archaeosine biosynthesis pathway, overview
-
metabolism
-
archaeosine biosynthesis pathway, overview
-
metabolism
-
archaeosine biosynthesis pathway, overview
-
physiological function
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
physiological function
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
physiological function
-
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
-
physiological function
-
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
-
physiological function
-
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
-
physiological function
-
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
-
physiological function
-
archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively. The TK1135 product is involved in G+ biosynthesis and q0kN is an intermediate in the synthesis of G+
-
additional information
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview
additional information
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview. Residues C51, C59 and C62 in the conserved CX3CX2C motif of radical SAM enzyme MaRaSEA are involved in G+ formation and coordinate iron atoms of the [4Fe-4S] cluster in MaRaSEA
additional information
-
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview. Residues C51, C59 and C62 in the conserved CX3CX2C motif of radical SAM enzyme MaRaSEA are involved in G+ formation and coordinate iron atoms of the [4Fe-4S] cluster in MaRaSEA
-
additional information
-
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview
-
additional information
-
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview. Residues C51, C59 and C62 in the conserved CX3CX2C motif of radical SAM enzyme MaRaSEA are involved in G+ formation and coordinate iron atoms of the [4Fe-4S] cluster in MaRaSEA
-
additional information
-
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview. Residues C51, C59 and C62 in the conserved CX3CX2C motif of radical SAM enzyme MaRaSEA are involved in G+ formation and coordinate iron atoms of the [4Fe-4S] cluster in MaRaSEA
-
additional information
-
the archaeosine synthases from Thermococcus kodakarensis is composed of ArcS (subunit alpha) and the radical SAM enzyme TkRaSEA (q0kN-tRNA lyase, subunit beta) both forming a robust complex, but the interaction between TkRaSEA and Thermoplasma acidophilum ArcS is considerably weaker. Interaction analysis, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C51A
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C51A-expressing cells
C59A
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C59A-expressing cells
C62A
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C62A-expressing cells
C51A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C51A-expressing cells
-
C59A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C59A-expressing cells
-
C62A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C62A-expressing cells
-
C51A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C51A-expressing cells
-
C59A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C59A-expressing cells
-
C62A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C62A-expressing cells
-
C51A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C51A-expressing cells
-
C59A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C59A-expressing cells
-
C62A
-
site-directed mutagenesis of a residue from the radical SAM enzymes' conserved CX3CX2C motif. No T1 fragment containing G+ is detected, but signals of U(s4U)AACAAA(q0kN)CGp are observed in the T1 fragments of tRNACys from C62A-expressing cells
-
additional information
construction of a TK1135 disruption strain (DELTATK1135). The G+ peak disappears from the tRNA fraction of strain DELTATK1135 and a small q0kN peak is observed
additional information
-
construction of a TK1135 disruption strain (DELTATK1135). The G+ peak disappears from the tRNA fraction of strain DELTATK1135 and a small q0kN peak is observed
-
additional information
-
construction of a TK1135 disruption strain (DELTATK1135). The G+ peak disappears from the tRNA fraction of strain DELTATK1135 and a small q0kN peak is observed
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.