ratio of N-acetylation of arylamines, O-aectylation of arylhydroxylamines and N,O-acetylation of arylhydroxamic acids, which are all performed by a single protein, may be highly dependent on the conformation of the enzyme
7-substituted-N-hydroxy-2-acetylamino fluorenes form adducts with DNA, which is inhibited by addition of ascorbic acid, 4-(2-biphenyl)-2-hydroxytetronic acid, and partly by N-tert-butyl-alpha-phenylnitrone, triethylphosphite, potassium O-ethylxanthate, thiourea, and N-acetylmethionine, not in case of 7-acetyl-N-hydroxy-2-acetylamino fluorene
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sitting and hanging drop methods, 20°C, 10 mg/ml protein solution: 10 mM Tris-HCl, pH 7.9, 0.1 M NaCl, 3 mM dithiothreitol, reservoir solution: 0.8 M Na,K tartrate, 0.1 M MES, pH 7.1, X-ray diffraction