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16-hydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 2-(16-hydroxyhexadecanoyl)-sn-glycerol 3-phosphate
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?
9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
dicarboxylic acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-dicarboxylic acyl-sn-glycerol 3-phosphate
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-
?
hexadecane-1,16-dioyl-CoA + sn-glycerol 3-phosphate
CoA + 2-hexadecane-1,16-dioyl-sn-glycerol 3-phosphate
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?
hexadecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 2-hexadecanoyl-sn-glycerol 3-phosphate
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-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-omega-hydroxy-acyl-sn-glycerol 3-phosphate
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-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-omega-hydroxy-acyl-sn-glycerol 3-phosphate
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-
?
sn-glycerol 3-phosphate + (9Z)-17-carboxyheptadec-9-enoyl-CoA
CoA + 1-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate + 2-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate
(9Z)-17-carboxyheptadec-9-enoyl-CoA = C18:1 DCA-CoA
((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate = C18:1 DCA-LPA, ratio of sn-2:sn-1-labeled products is 5:1 or greater
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?
sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
H9XTI6; H9XTI4; H9XTI5
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reaction mainly occurs in sn-2 position. Intrinsic phosphatase activity of the enzymes finally leads to formation of 2-(15-carboxypentadecanoyl)-sn-glycerol
-
?
sn-glycerol 3-phosphate + palmitoyl-CoA
CoA + 2-palmitoyl-sn-glycerol 3-phosphate
H9XTI6; H9XTI4; H9XTI5
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phosphatase activity of the enzymes does not act on non-substituted acyl residues
-
?
additional information
?
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9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate
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-
-
?
9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate
the principal putative substrate of SlGPAT6 is 9(10),16-dihydroxyhexadecanoic acid (C16:0 diOH) is the major cutin monomer present in tomato fruit cuticles
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-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
isozyme GPAT4 acylates glycerol-3-phosphate at the sn-2 position
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?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
isozyme GPAT8 acylates glycerol-3-phosphate at the sn-2 position
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-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
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-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
isozyme GPAT4 acylates glycerol-3-phosphate at the sn-2 position
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-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
isozyme GPAT8 acylates glycerol-3-phosphate at the sn-2 position
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-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
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-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
15-carboxypentadecanoyl-CoA = C16:0 DCA-CoA
(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate = C16:0 DCA-LPA, ratio of sn-2:sn-1-labeled products is 5:1 or greater
-
?
sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
15-carboxypentadecanoyl-CoA = C16:0 DCA-CoA
(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate = C16:0 DCA-LPA, sn-2:sn-1-labeled products is 2:1
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?
additional information
?
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omega-hydroxy-acyl-CoA is the preferred substrate compared to dicarboxylic acyl-CoA. The isozyme shows a regiospecificity that prefers sn-2 before sn-1
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additional information
?
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omega-hydroxy-acyl-CoA is the preferred substrate compared to dicarboxylic acyl-CoA. The isozyme shows a regiospecificity that prefers sn-2 before sn-1
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additional information
?
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the isozyme prefers dicarboxylic acyl-CoA before omega-hydroxy acyl-CoA and acyl-CoA as substrates. The isozyme shows a regiospecificity that prefers sn-2 before sn-1
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additional information
?
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the isozyme prefers dicarboxylic acyl-CoA before omega-hydroxy acyl-CoA and acyl-CoA as substrates. The isozyme shows a regiospecificity that prefers sn-2 before sn-1
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additional information
?
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H9XTI6; H9XTI4; H9XTI5
all three Gpat4 isoforms exhibit sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested
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?
additional information
?
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all three Gpat4 isoforms exhibit sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested
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?
additional information
?
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analysis of SlGPAT6 substrate specificity of wild-type and mutant enzymes, overview
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9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate
the principal putative substrate of SlGPAT6 is 9(10),16-dihydroxyhexadecanoic acid (C16:0 diOH) is the major cutin monomer present in tomato fruit cuticles
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-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
dicarboxylic acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-dicarboxylic acyl-sn-glycerol 3-phosphate
-
-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-omega-hydroxy-acyl-sn-glycerol 3-phosphate
-
-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-omega-hydroxy-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
-
-
-
?
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evolution
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occurrence of land-plant-specific glycerol-3-phosphate acyltransferases is essential for cuticle formation and gametophore development in Physcomitrella patens, sn-2 GPATs harboring both domains by gene recombination are critical to cuticle formation of early land plants. Phylogenetic analysis
malfunction
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a loss-of-function mutation in tomato SlGPAT6 results in increased susceptibility of leaves to Phytophthora infection, concomitant with changes in haustoria morphology. Modulation of GPAT6 expression alters the outer wall diameter of leaf epidermal cells. Tomato gpat6-a mutants have an impaired cell wall-cuticle continuum and fewer stomata, but show increased water loss
malfunction
glycerol content is strongly reduced in both stem and leaf cutin from all Arabidopsis thaliana mutants analyzed (gpat4/gpat8). Knocking out any of these genes individually, or as a pair (gpat4/gpat8), leads to large reductions in Arabidopsis thaliana cutin monomer loads, especially in the major monomer octadeca-6,9-diene-1,18-dioate (C18:2 DCA). sn-2-GPATs play pivotal roles in providing glycerol as a structural monomer for cutin through regiospecific sn-2 acylation of glycerol-3-phosphate. The double-knockout gpat4/gpat8 has an overall 60-70% reduction of aliphatic monomers in stem and leaf cutin, and the most pronounced decrease occurs in its major monomer, C18:2 dicarboxylic acyl-CoA (DCA)
malfunction
GPAT6 mutant seeds show reduced germination rates and root lengths compared to wild-type in presence of 50-150 mM salt. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT6. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of phosphatidylglycerol (PG)
malfunction
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silencing of Nicotiana benthamiana NbGPAT6a increases leaf susceptibility to infection by the oomycetes Phytophthora infestans and Phytophthora palmivora, whereas overexpression of NbGPAT6a-GFP renders the leaves more resistant. Knockdown or knockout of GPAT6 renders leaves more susceptible to Phytophthora infection but more resistant to Bortrytis cinerea infection
malfunction
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targeted disruption of PpGPAT2 delays growth and gametophore development. Disruption of PpGPAT2 causes increased susceptibility to osmotic and salt stresses in Physcomitrella patens
malfunction
the G163R mutation in gpat6-a confers a loss of SlGPAT6-mediated GPAT activity leading to P23F12 glossy mutant line with with abnormal fruit cutin amounts/compositions and increased fruit brightness. Loss of SlGPAT6 function leads to altered expression of genes involved in cuticle and cell wall formation and remodeling
malfunction
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glycerol content is strongly reduced in both stem and leaf cutin from all Arabidopsis thaliana mutants analyzed (gpat4/gpat8). Knocking out any of these genes individually, or as a pair (gpat4/gpat8), leads to large reductions in Arabidopsis thaliana cutin monomer loads, especially in the major monomer octadeca-6,9-diene-1,18-dioate (C18:2 DCA). sn-2-GPATs play pivotal roles in providing glycerol as a structural monomer for cutin through regiospecific sn-2 acylation of glycerol-3-phosphate. The double-knockout gpat4/gpat8 has an overall 60-70% reduction of aliphatic monomers in stem and leaf cutin, and the most pronounced decrease occurs in its major monomer, C18:2 dicarboxylic acyl-CoA (DCA)
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malfunction
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GPAT6 mutant seeds show reduced germination rates and root lengths compared to wild-type in presence of 50-150 mM salt. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT6. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of phosphatidylglycerol (PG)
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metabolism
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cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position)
metabolism
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cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position)
metabolism
isozymes GPAT4 and GPAT8 are glycerol-3-phosphate 2-O acyltransferases that catalyze the first step in leaf and stem cutin acylglycerol assembly
metabolism
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isozymes GPAT4 and GPAT8 are glycerol-3-phosphate 2-O acyltransferases that catalyze the first step in leaf and stem cutin acylglycerol assembly
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physiological function
The enzyme is involved in cutin and suberin biosynthesis in plants. The common acyl monomer substrates (as CoA esters) are omega-hydroxy fatty acids (omega-OHFAs) and alpha,omega-dicarboxylic acids (DCAs). Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols (2-MAGs).
physiological function
The enzyme is involved in cutin and suberin biosynthesis in plants. The common acyl monomer substrates (as CoA esters) are omega-hydroxy fatty acids and alpha,omega-dicarboxylic acids. Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols (2-MAGs).
physiological function
H9XTI6; H9XTI4; H9XTI5
RNAi-mediated down-regulation of all Gpat4 homologues causes abnormal development of several reproductive organs and reduced seed set. Both pollen grains and developing embryo sacs of the downregulated Gpat4 lines are affected. The gpat4 mature embryos show decreased cutin content and altered monomer composition. The defective embryo development affects the oil body morphology, oil content, and fatty acid composition in Gpat4-lacking seeds
physiological function
glycerol-3-phosphate 2-O-acyltransferases (sn-2-GPATs) are essential for cutin biosynthesis and involved in cutin polymerization, cutin structures and compositions in plant tissues, detailed overview
physiological function
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glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of Nicotiana benthamiana leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration
physiological function
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glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration
physiological function
glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis. Wild-type SlGPAT6 has the dual acyltransferase and phosphatase activities expected from a GPAT enzyme involved in cutin monomer biosynthesis
physiological function
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glycerol-3-phosphate acyltransferases is essential for cuticle formation and gametophore development in Physcomitrella patens. Glycerol-3-phosphate acyltransferase (GPAT) harboring bifunctional sn-2 acyltransferase/phosphatase activity produces 2-monoacylglycerol, a precursor for cutin synthesis. The cutin layer plays an important role in plant organ formation by providing organ boundaries. Floral organ fusion and permeable cuticle phenotypes of Arabidopsis thaliana mutant gpat6-2 petals are rescued to the wild-type by the expression of PpGPAT2 or PpGPAT4
physiological function
plant sn-glycerol-3-phosphate acyltransferases are biocatalysts involved in the biosynthesis of intracellular and extracellular lipids. Isozyme GPAT4 plays a role in leaf and stem cutin biosynthetic processes and phospholipid biosynthetic process (functionally redundant with GPAT8)
physiological function
plant sn-glycerol-3-phosphate acyltransferases are biocatalysts involved in the biosynthesis of intracellular and extracellular lipids. Isozyme GPAT6 plays a role in flower cutin biosynthetic process, flower development, and the phospholipid biosynthetic process
physiological function
the enzyme is involved in cutin polymerization, cutin structures and compositions in plant tissues, detailed overview
physiological function
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glycerol-3-phosphate 2-O-acyltransferases (sn-2-GPATs) are essential for cutin biosynthesis and involved in cutin polymerization, cutin structures and compositions in plant tissues, detailed overview
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physiological function
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the enzyme is involved in cutin polymerization, cutin structures and compositions in plant tissues, detailed overview
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additional information
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constitutive expression of NbGPAT6a renders leaves resistant to Phytophthora infection, NbGPAT6-mediated resistance is associated with longer-term leaf development processes. Modulating GPAT6 expression alters the thickness of the outer cell walls of the leaf epidermis
additional information
structural modeling of SlGPAT6, based on the Methanococcus jannaschii phospho-Ser phosphatase template and restricted to the N-terminal 17 to 207 amino acid region, generated in silico, revealing that the G-to-R amino acid substitution at position 163 is close to the cluster of residues that are essential for the catalytic activity and Mg2+ binding of GPAT6
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G163R
isolation of a set of fruit brightness mutants (named glossy mutants) with abnormal fruit cutin amounts/compositions from the EMS-mutagenized tomato mutant population (cv Micro-Tom). The glossy mutant line P23F12 exhibits enhanced fruit brightness and a reduced cutin load. Whole-genome sequencing and gene mapping show that a single G613A nucleotide transition in the first exon leading to a G163R nonsynonymous mutation in gene SlGPAT (Solyc09g014350) is responsible for the mutant glossy fruit phenotype, i.e. mutant gpat6-a, overview. The inactive mutant gpat6-a has a fruit cuticle that shows reduced levels of C16 cutin monomers. No differences are observed in total wax load between wild-type and gpat6-a fruits, but differences in wax composition are apparent, such as increased or decreased levels of several minor C25 to C30 alkanes in the gpat6-a mutant. Since both even- and odd-numbered alkanes display similar trends, these variations are likely independent of the alkane biosynthetic pathways. The most striking wax compositional change in the gpat6-a mutant is an approximately 5fold increase in delta- and alpha-amyrin levels and to a lesser extent in beta-amyrin levels. In contrast to waxes, the total cutin load in gpat6-a is reduced by almost 3fold. MapMan-based general overview of genes differentially expressed between wild-type and gpat6-a mutant fruit
additional information
generation of a T-DNA insertion mutant strain of GPAT6, determination of chlorophyll content and fatty acids composition of phosphatidylglycerol (PG) in Arabidopsis thaliana GPAT6 mutant line, overview. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT6. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of PG
additional information
-
glycerol content is strongly reduced in both stem and leaf cutin from all Arabidopsis thaliana mutants analyzed (gpat4/gpat8). Knocking out of these genes individually or as a pair (gpat4/gpat8) leads to large reductions in Arabidopsis thaliana cutin monomer loads, especially in the major monomer octadeca-6,9-diene-1,18-dioate (C18:2 DCA)
additional information
glycerol content is strongly reduced in both stem and leaf cutin from all Arabidopsis thaliana mutants analyzed (gpat4/gpat8). Knocking out of these genes individually or as a pair (gpat4/gpat8) leads to large reductions in Arabidopsis thaliana cutin monomer loads, especially in the major monomer octadeca-6,9-diene-1,18-dioate (C18:2 DCA)
additional information
-
glycerol content is strongly reduced in both stem and leaf cutin from all Arabidopsis thaliana mutants analyzed (gpat4/gpat8). Knocking out of these genes individually or as a pair (gpat4/gpat8) leads to large reductions in Arabidopsis thaliana cutin monomer loads, especially in the major monomer octadeca-6,9-diene-1,18-dioate (C18:2 DCA)
-
additional information
-
generation of a T-DNA insertion mutant strain of GPAT6, determination of chlorophyll content and fatty acids composition of phosphatidylglycerol (PG) in Arabidopsis thaliana GPAT6 mutant line, overview. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT6. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of PG
-
additional information
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gene GPAT6 knockout phenotype, overview
additional information
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targeted disruption of PpGPAT2, DELTAppgpat2 phenotype, overview
additional information
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targeted mutagenesis of PpGPAT4, DELTAppgpat4 phenotype, overview
additional information
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leaves of 6-wk-old tomato cv. Micro-Tom or gpat6-a mutant plants are subjected to a detached leaf infection assay, gpat6-a tomato fruits have increased cuticle permeability, gpat6-a tomato leaves have fewer stomata and show an increased rate of water loss compared with the wild-type. Mutant gpat6-a leaf epidermal cells possess a thicker cell wall. The gpat6-a leaf transcriptome reflects changes in cuticle and cell wall processes and stomatal patterning. Phenotype, overview
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cloned in yeast microsomes and wheat germ cells
gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis
gene GPAT6, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis
gene SlGPAT6, located on chromosome 9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of SlGPAT6 wild-type and DELTASlGPAT6 mutant carrying the G163R mutation in a Saccharomyces cerevisiae gat1DELTA mutant strain with a GAT1 knockout
phylogenetic analysis, recombinant expression of eYFP-tagged GPAT3 in Nicotiana benthamiana leaves via transfection by Agrobacterium tumefaciens strain GV3101, functional overexpression under control of the CaMV35S promoter in and complementation of Arabidopsis thaliana mutant gpat6-2
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phylogenetic analysis, recombinant expression of eYFP-tagged GPAT4 in Nicotiana benthamiana leaves via transfection by Agrobacterium tumefaciens strain GV3101, functional overexpression under control of the CaMV35S promoter in and complementation of Arabidopsis thaliana mutant gpat6-2
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gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis
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gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis
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Yang, W.; Pollard, M.; Li-Beisson, Y.; Beisson, F.; Feig, M.; Ohlrogge, J.
A distinct type of glycerol-3-phosphate acyltransferase with sn-2 preference and phosphatase activity producing 2-monoacylglycerol
Proc. Natl. Acad. Sci. USA
107
12040-12045
2010
Arabidopsis thaliana (O80437), Arabidopsis thaliana (Q9CAY3), Arabidopsis thaliana (Q9LMM0)
brenda
Chen, X.; Chen, G.; Truksa, M.; Snyder, C.L.; Shah, S.; Weselake, R.J.
Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
J. Exp. Bot.
65
4201-4215
2014
Brassica napus (H9XTI6 and H9XTI4 and H9XTI5), Brassica napus
brenda
Sui, N.; Tian, S.; Wang, W.; Wang, M.; Fan, H.
Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis
Front. Plant Sci.
8
1337
2017
Arabidopsis thaliana (O80437), Arabidopsis thaliana Col-0 (O80437)
brenda
Jayawardhane, K.N.; Singer, S.D.; Weselake, R.J.; Chen, G.
Plant sn-glycerol-3-phosphate acyltransferases biocatalysts involved in the biosynthesis of intracellular and extracellular lipids
Lipids
53
469-480
2018
Arabidopsis thaliana (O80437), Arabidopsis thaliana (Q9LMM0)
brenda
Fawke, S.; Torode, T.A.; Gogleva, A.; Fich, E.A.; S?rensen, I.; Yunusov, T.; Rose, J.K.C.; Schornack, S.
Glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato and Nicotiana benthamiana leaf epidermis
New Phytol.
223
1547-1559
2019
Solanum lycopersicum, Nicotiana benthamiana
brenda
Lee, S.B.; Yang, S.U.; Pandey, G.; Kim, M.S.; Hyoung, S.; Choi, D.; Shin, J.S.; Suh, M.C.
Occurrence of land-plant-specific glycerol-3-phosphate acyltransferases is essential for cuticle formation and gametophore development in Physcomitrella patens
New Phytol.
225
2468-2483
2020
Physcomitrium patens
brenda
Yang, W.; Pollard, M.; Li-Beisson, Y.; Ohlrogge, J.
Quantitative analysis of glycerol in dicarboxylic acid-rich cutins provides insights into Arabidopsis cutin structure
Phytochemistry
130
159-169
2016
Arabidopsis thaliana, Arabidopsis thaliana (Q5XF03), Arabidopsis thaliana Col-0, Arabidopsis thaliana Col-0 (Q5XF03)
brenda
Petit, J.; Bres, C.; Mauxion, J.P.; Tai, F.W.; Martin, L.B.; Fich, E.A.; Joubes, J.; Rose, J.K.; Domergue, F.; Rothan, C.
The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis
Plant Physiol.
171
894-913
2016
Solanum lycopersicum (A0A3Q7I0S2)
brenda