Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acetyl-CoA + L-2,4-diaminobutanoate
CoA + (2S)-4-acetamido-2-aminobutanoate
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
propionyl-CoA + L-2,4-diaminobutanoate
CoA + N4-propionyl-L-2,4-diaminobutanoate
additional information
?
-
acetyl-CoA + L-2,4-diaminobutanoate
CoA + (2S)-4-acetamido-2-aminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + (2S)-4-acetamido-2-aminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + (2S)-4-acetamido-2-aminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + (2S)-4-acetamido-2-aminobutanoate
the enzyme catalyzes a step in ectoine biosynthesis
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
enzyme is involved in ectoine biosynthesis pathway
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
enzyme is involved in ectoine biosynthesis pathway
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
together EctA, EctB and EctC constitute the ectoine biosynthetic pathway. ectABC genes are genetically organized as an operon whose expression is strongly enhanced when the osmolarity of the growth medium is raised
-
-
?
propionyl-CoA + L-2,4-diaminobutanoate
CoA + N4-propionyl-L-2,4-diaminobutanoate
-
20% of the activity with acetyl-CoA
-
-
?
propionyl-CoA + L-2,4-diaminobutanoate
CoA + N4-propionyl-L-2,4-diaminobutanoate
-
20% of the activity with acetyl-CoA
-
-
?
additional information
?
-
-
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
-
-
?
additional information
?
-
-
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
-
-
?
additional information
?
-
-
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
-
-
?
additional information
?
-
-
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
-
-
?
additional information
?
-
-
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acetyl-CoA + L-2,4-diaminobutanoate
CoA + (2S)-4-acetamido-2-aminobutanoate
the enzyme catalyzes a step in ectoine biosynthesis
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
enzyme is involved in ectoine biosynthesis pathway
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
enzyme is involved in ectoine biosynthesis pathway
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
together EctA, EctB and EctC constitute the ectoine biosynthetic pathway. ectABC genes are genetically organized as an operon whose expression is strongly enhanced when the osmolarity of the growth medium is raised
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
crystal structures of the enzyme in complex with the cosubstrate reveals an evolutionarily conserved CoA-binding site
evolution
MH020162
phylogenetic analysis of nucleotides and amino acids reveals that the ectA, B and C sequences of Bacillus clausii NIOT-DSB04 were conserved in many eubacteria
evolution
-
phylogenetic analysis of nucleotides and amino acids reveals that the ectA, B and C sequences of Bacillus clausii NIOT-DSB04 were conserved in many eubacteria
-
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview. EctR1 negatively controls transcription of the ect genes, EctR1 function, detailed overview. But Methylomicrobium alcaliphilum may possessseveral regulatory systems
metabolism
the enzyme catalyzes a step in ectoine biosynthesis
metabolism
MH020162
the enzyme is involved in ectoine biosynthesis. Ectoine is a compatible solute, serves as a protective compound in many halophilic eubacterial cells under stress
metabolism
-
the enzyme is involved in ectoine biosynthesis. Ectoine is a compatible solute, serves as a protective compound in many halophilic eubacterial cells under stress
-
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis
-
metabolism
-
the enzyme is involved in the pathway of ectoine biosynthesis from aspartic acid, overview
-
additional information
-
osmoregulated expression of ectABC, and regulatory function of EctR1 in ectoine biosynthesis
additional information
-
regulation of expression of ectABC operon, osmotic up-shock of Halobacillus halophilus cells leads to the enhanced levels of mRNAs of ectABC, overview. Ectoine/proline ratio that is low in cells growing exponentially at different salinities, of 2 and 3 M NaCl, enhances more than 1000fold in the stationary growth phase
additional information
-
regulation of expression of ectABC operon, overview
additional information
-
regulation of expression of ectABC operon, overview
additional information
-
regulation of expression of ectABC operon, overview. In Gram-positive bacteria, ectoine biosynthesis also is triggered by an osmotic stress
additional information
-
regulation of expression of ectABC operon, overview. In Gram-positive bacteria, ectoine biosynthesis also is triggered by an osmotic stress
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-70C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
4°C or -70°C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl
-
4C, 50 mM Tris-HCl, pH 8.5, 1 month
4-70C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
-
4-70C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
-
4-70C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
-
4C, 50 mM Tris-HCl, pH 8.5, 1 month
4C, 50 mM Tris-HCl, pH 8.5, 1 month
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cloned into the expression vector pQE30 with a 6×histidine tag and expressed in Escherichia coli JM109 M15 cells
MH020162
expression in Escherichia coli
-
expression in Escherichia coli with an additional six His residues at the C-terminus
-
expression in Escherichia coli, also osmoregulated in Escherichia coli
-
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
gene ectA, ectABC operon, in Marinococcus halophilus, the transcription of ectABC genes is initiated from three individual sigma70/sigmaA-promoters locate upstream of each gene
-
gene ectA, encoded in ectABC, transcription of the ectABC-ask operon is initiated from two sigma70-dependent promoters ectAp1 and ectAp2. Gene ectR1 encodes the MarR-like transcriptional regulator EctR that is involved in the regulation of ectABC, overview
-
heterologous production of EctA in Escherichia coli, with either a N-terminal (NH2-WSHPQFEK-SG) or a C-terminal (SA-WSHPQFEK-COOH) Strep-Tag II peptide for their purification by affinity chromatography
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
our putative promoters (?ectA1-4) are located upstream of the ectA gene and one internal promoter PectB is upstream of the ectB gene
-
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Peters, P.; Galinski, E.A.; Trper, H.G.
The biosynthesis of ectoine
FEMS Microbiol. Lett.
71
157-162
1990
Halorhodospira halochloris
-
brenda
Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.
Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata
J. Bacteriol.
181
91-99
1999
Halomonas elongata, Halomonas elongata OUT30018
brenda
Kuhlmann, A.U.; Bremer, E.
Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp.
Appl. Environ. Microbiol.
68
772-783
2002
Sporosarcina pasteurii
brenda
Reshetnikov, A.S.; Khmelenina, V.N.; Trotsenko, Y.A.
Characterization of the ectoine biosynthesis genes of haloalkalotolerant obligate methanotroph "Methylomicrobium alcaliphilum 20Z"
Arch. Microbiol.
184
286-297
2006
Methylotuvimicrobium alcaliphilum, Methylotuvimicrobium alcaliphilum 20Z
brenda
Reshetnikov, A.S.; Mustakhimov, II; Khmelenina, V.N.; Trotsenko, Y.A.
Cloning, purification, and characterization of diaminobutyrate acetyltransferase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z
Biochemistry (Moscow)
70
878-883
2005
Methylotuvimicrobium alcaliphilum, Methylotuvimicrobium alcaliphilum 20Z
brenda
Louis, P.; Galinski, E.A.
Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli
Microbiology
143
1141-1149
1997
Marinococcus halophilus
brenda
Mustakhimov, I.I.; Rozova, O.N.; Reshetnikov, A.S.; Khmelenina, V.N.; Murrell, J.C.; Trotsenko, Y.A.
Characterization of the recombinant diaminobutyric acid acetyltransferase from Methylophaga thalassica and Methylophaga alcalica
FEMS Microbiol. Lett.
283
91-96
2008
Methylophaga thalassica (A9YZX5), Methylophaga thalassica, Methylophaga alcalica (A9YZY0), Methylophaga alcalica
brenda
Khmelenina, V.; Mustakhimov, I.; Reshetnikov, A.; Kalyuzhnaya, M.; Trotsenko, Y.
Genetic and biochemical aspects of ectoine biosynthesis in moderately halophilic and halotolerant methylotrophic bacteria
Am. J. Agric. Biol. Sci.
5
446-458
2010
Sporosarcina pasteurii, Halomonas elongata, Methylophaga thalassica, Marinococcus halophilus, Methylotuvimicrobium alcaliphilum, Chromohalobacter salexigens, Halobacillus halophilus, Methylophaga alcalica, Brevibacterium epidermidis, Halomonas elongata KS3
-
brenda
Reshetnikov, A.S.; Khmelenina, V.N.; Mustakhimov, I.I.; Trotsenko, Y.A.
Genes and enzymes of ectoine biosynthesis in halotolerant methanotrophs
Methods Enzymol.
495
15-30
2011
Halomonas elongata, Methylophaga thalassica, Methylotuvimicrobium alcaliphilum, Methylophaga alcalica, Methylotuvimicrobium alcaliphilum 20Z
brenda
Anburajan, L.; Meena, B.; Vinithkumar, N.; Kirubagaran, R.; Dharani, G.
Functional characterization of a major compatible solute in Deep Sea halophilic eubacteria of active volcanic Barren Island, Andaman and Nicobar Islands, India
Infect. Genet. Evol.
73
261-265
2019
Alkalihalobacillus clausii (MH020162), Alkalihalobacillus clausii NIOT-DSB04 (MH020162)
brenda
Richter, A.A.; Kobus, S.; Czech, L.; Hoeppner, A.; Zarzycki, J.; Erb, T.J.; Lauterbach, L.; Dickschat, J.S.; Bremer, E.; Smits, S.H.J.
The architecture of the diaminobutyrate acetyltransferase active site provides mechanistic insight into the biosynthesis of the chemical chaperone ectoine
J. Biol. Chem.
295
2822-2838
2020
Paenibacillus lautus (A0A385TZ63), Paenibacillus lautus
brenda