Information on EC 1.9.98.1 - iron-cytochrome-c reductase

for references in articles please use BRENDA:EC1.9.98.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Acidithiobacillus ferrooxidans

top print hide
EC NUMBER
COMMENTARY hide
1.9.98.1
-
top print hide
RECOMMENDED NAME
GeneOntology No.
iron-cytochrome-c reductase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ferrocytochrome c + Fe3+ = ferricytochrome c + Fe2+
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
ferrocytochrome-c:Fe3+ oxidoreductase
An iron protein.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
37256-52-3
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ferrocytochrome c + Fe3+
ferricytochrome c + Fe2+
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ferrocytochrome c + Fe3+
ferricytochrome c + Fe2+
show the reaction diagram
-
close association of enzyme with respiration chain
-
-
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome
-
most highly purified fraction of the enzyme contains cytochrome c and apparently nonfunctional cytochrome b
-
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxo-3-phenylpropanoate
-
100% inhibition at 0.08 mM
Cr2+
-
slight inhibition
Cu2+
-
100% inhibition at 0.1 mM
cysteine
-
100% inhibition at 0.2 mM
Fe3+
-
48% inhibition at 1 mM
Ni2+
-
slight inhibition
p-hydroxymercuribenzoate
-
100% inhibition at 0.5 mM, inhibition can be reversed by reduced glutathione or cysteine
reduced glutathione
-
67% inhibition at 1 mM
additional information
-
not inhibitory: atebrin, amytal, antimycin A
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0275
cytochrome c
-
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.7
-
pH 5: about 50% of maximum activity, pH 6.7: about 25% of maximum activity
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
-
several weeks, no loss of activity
22
-
8 h, no loss of activity
40
-
20 min, 10% loss of activity
60
-
5 min, 46% loss of activity
90
-
1 min, 74% loss of activity
100
-
1 min, complete loss of activity
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-5°C, several weeks without loss of activity
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.9.98.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)