Information on EC 1.8.98.6 - formate:CoB-CoM heterodisulfide,ferredoxin reductase

for references in articles please use BRENDA:EC1.8.98.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Methanococcus maripaludis

top print hide
EC NUMBER
COMMENTARY hide
1.8.98.6
-
top print hide
RECOMMENDED NAME
GeneOntology No.
formate:CoB-CoM heterodisulfide,ferredoxin reductase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 CO2 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ = 2 formate + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
show the reaction diagram
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme B/coenzyme M regeneration V (formate-dependent)
-
-
Methane metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
coenzyme B,coenzyme M,ferredoxin:formate oxidoreductase
-
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the protein complex consists of heterodisulfide reductase, formylmethanofuran dehydrogenase, F420-nonreducing hydrogenase, and formate dehydrogenase. Either H2 or formate can donate electrons to the heterodisulfide-H2 via F420-nonreducing hydrogenase or formate via formate dehydrogenase. When H2 is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase via F420-nonreducing hydrogenase. Flavin-mediated electron bifurcation at heterodisulfide reductase A then results in reduction of the CoM-S-S-CoB heterodisulfide and a ferredoxin that is used by formylmethanofuran dehydrogenase for the first step in methanogenesis. When hydrogen is limiting or is replaced by formate, formate dehydrogenase is highly expressed and incorporates into the complex. When formate is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase from formate via formate dehydrogenase
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
show the reaction diagram
-
-
-
-
?
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
500000
-
gel filtration
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+ resin column chromatography
-
nickel affinity column chromatography and Hiprep 16-60 Sephacryl S300HR gel filtration
-
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.8.98.6)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)