Information on EC 1.8.5.8 - eukaryotic sulfide quinone oxidoreductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.5.8
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RECOMMENDED NAME
GeneOntology No.
eukaryotic sulfide quinone oxidoreductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrogen sulfide + glutathione + a quinone = S-sulfanylglutathione + a quinol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfide oxidation IV (metazoa)
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Sulfur metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
sulfide:glutathione,quinone oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GSH + coenzyme Q1
GSSG + reduced coenzyme Q1
show the reaction diagram
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hydrogen sulfide + coenzyme Q1
hydrogen disulfide + reduced coenzyme Q1
show the reaction diagram
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?
hydrogen sulfide + coenzyme Q2
?
show the reaction diagram
hydrogen sulfide + cysteine + coenzyme Q1
?
show the reaction diagram
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?
hydrogen sulfide + glutathione + a quinone
S-sulfanylglutathione + a quinol
show the reaction diagram
hydrogen sulfide + glutathione + coenzyme Q
S-sulfanylglutathione + reduced coenzyme Q
show the reaction diagram
hydrogen sulfide + glutathione + coenzyme Q1
?
show the reaction diagram
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?
hydrogen sulfide + glutathione + coenzyme Q1
glutathione persulfide + H+ + reduced coenzyme Q1
show the reaction diagram
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?
hydrogen sulfide + glutathione + coenzyme Q1
S-sulfanylglutathione + reduced coenzyme Q1
show the reaction diagram
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?
hydrogen sulfide + glutathione + coenzyme Q10
S-sulfanylglutathione + reduced coenzyme Q10
show the reaction diagram
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?
hydrogen sulfide + homocysteine + coenzyme Q1
?
show the reaction diagram
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?
hydrogen sulfide + sulfide + coenzyme Q
?
show the reaction diagram
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?
hydrogen sulfide + sulfide + coenzyme Q1
?
show the reaction diagram
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?
hydrogen sulfide + sulfite + coenzyme Q
?
show the reaction diagram
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?
hydrogen sulfide + sulfite + coenzyme Q1
thiosulfate + reduced coenzyme Q1
show the reaction diagram
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?
sulfide + coenzyme Q2
?
show the reaction diagram
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?
sulfide + ubiquinone
?
show the reaction diagram
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?
additional information
?
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no activity with DHLA, cysteamine, coenzyme A, hypotaurine, cysteine sulfinic acid, and thioredoxin
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hydrogen sulfide + glutathione + a quinone
S-sulfanylglutathione + a quinol
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
H2S
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the enzyme activity decreases when the ambient sulfide concentration exceeds 0.3 mM
Zn2+
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80.32% residual activity at 5 mM
additional information
not inhibited by up to 2 mM H2S
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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109.33% activity at 5 mM
glutathione
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110.52% activity at 5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 3
cysteine
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at pH 7.4 and 37C
0.008 - 22
glutathione
22
homocysteine
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at pH 7.4 and 37C
0.00287 - 2
hydrogen sulfide
0.005 - 0.35
Sulfide
0.19 - 0.26
sulfite
0.0156
ubiquinone
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at pH 8.5 and 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
89 - 128
glutathione
62 - 373
Sulfide
382 - 650
sulfite
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.1
cysteine
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at pH 7.4 and 37C
5.1 - 16
glutathione
4.2
homocysteine
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at pH 7.4 and 37C
23.1 - 29000
Sulfide
1700 - 2500
sulfite
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.12
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at pH 8.0 and 25C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9
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more than 70% activity between pH 8.0 and 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27 - 42
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more than 60% activity between 27 and 42C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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highest expression
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography and Mono Q column chromatography
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Ni-NTA column chromatography
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nickel chelate resin column chromatography and DEAE-Sephadex gel filtration
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Resource Q column chromatography, Source S column chromatography, and Superdex 75 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli XL1-Blue cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression increases significantly at 12 h in 0.05 mM sulfide and 6 h in 0.15 mM sulfide and then continues to increase with time
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