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Information on EC 1.8.2.7 - thiocyanate desulfurase
for references in articles please use BRENDA:EC1.8.2.7
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EC Tree 1 Oxidoreductases
1.8 Acting on a sulfur group of donors
1.8.2 With a cytochrome as acceptor
1.8.2.7 thiocyanate desulfurase
IUBMB Comments
The enzyme, characterized from the haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio paradoxus, contains three copper ions in its active site. It catalyses the direct conversion of thiocyanate into cyanate and elemental sulfur without involvement of molecular oxygen.
The enzyme appears in viruses and cellular organisms
- 1.8.2.7
- thioalkalivibrio
-
hydratase
- scn
-
haloalkaliphilic
-
sulfur-oxidizing
-
halophil
- copper
-
metagenomic
Reaction Schemes
show+
2
+
=
+
+
2
+
2
Synonyms
thiocyanate dehydrogenase, thiocyanate desulfurase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
TcDH
thiocyanate dehydrogenase
thiocyanate: cytochrome c oxidoreductase
TcDH
Thioalkalivibrio thiocyanoxidans ARh 2T
-
-
-
thiocyanate dehydrogenase
-
-
-
-
thiocyanate dehydrogenase
Thioalkalivibrio thiocyanoxidans ARh 2T
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
thiocyanate + 2 ferricytochrome c + H2O = cyanate + sulfur + 2 ferrocytochrome c + 2 H+
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
thiocyanate degradation I
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SYSTEMATIC NAME
IUBMB Comments
thiocyanate:cytochrome c oxidoreductase (cyanate and sulfur-forming)
The enzyme, characterized from the haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio paradoxus, contains three copper ions in its active site. It catalyses the direct conversion of thiocyanate into cyanate and elemental sulfur without involvement of molecular oxygen.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
thiocyanate + 2 potassium ferricyanide + H2O
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
thiocyanate + 2 ferricyanide + H2O
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricyanide + H2O
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c + H2O
cyanate + sulfur + 2 ferrocytochrome c + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c + H2O
cyanate + sulfur + 2 ferrocytochrome c + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c + H2O
cyanate + sulfur + 2 ferrocytochrome c + 2 H+
Thioalkalivibrio thiocyanoxidans ARh 2T
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 potassium ferricyanide + H2O
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 potassium ferricyanide + H2O
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 potassium ferricyanide + H2O
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 potassium ferricyanide + H2O
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 potassium ferricyanide + H2O
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + Wurster's blue + H2O
cyanate + sulfur + reduced Wurster's blue + H+
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
Products: -
Products: -
-
additional information
?
-
-
Substrates: low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
Products: -
Products: -
-
additional information
?
-
-
Substrates: low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
Products: -
Products: -
-
additional information
?
-
-
Substrates: low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
Products: -
Products: -
-
additional information
?
-
-
Substrates: low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
Products: -
Products: -
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
thiocyanate + 2 ferricyanide + H2O
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricyanide + H2O
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c + H2O
cyanate + sulfur + 2 ferrocytochrome c + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c + H2O
cyanate + sulfur + 2 ferrocytochrome c + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c + H2O
cyanate + sulfur + 2 ferrocytochrome c + 2 H+
Thioalkalivibrio thiocyanoxidans ARh 2T
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
thiocyanate + 2 ferricytochrome c 550 + H2O
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
Substrates: -
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Cu2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM
-
additional information
-
nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
9.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8 - 11
-
more than 50% activity between pH 8.0 and 11.0 with potassium ferricyanide as electron acceptor, more than 50% activity between pH 8.5 and 10.5 with potassium ferricyanide as electron acceptor
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
?
Thioalkalivibrio thiocyanoxidans ARh 2T
-
x * 56000, SDS-PAGE
-
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M sodium citrate, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol or 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% (w/v) PEG 8000, 15% (v/v) glycerol
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tsallagov, S.I.; Sorokin, D.Y.; Tikhonova, T.V.; Popov, V.O.; Muyzer, G.
Comparative genomics of Thiohalobacter thiocyanaticus HRh1T and Guyparkeria sp. SCN-R1, halophilic chemolithoautotrophic sulfur-oxidizing gammaproteobacteria capable of using thiocyanate as energy source
Front. Microbiol.
10
898
2019
Guyparkeria sp. SCN-R1, Thiohalobacter thiocyanaticus
brenda
Berben, T.; Balkema, C.; Sorokin, D.; Muyzer, G.
Analysis of the genes involved in thiocyanate oxidation during growth in continuous culture of the haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio thiocyanoxidans ARh 2T using transcriptomics
mSystems
2
00102-17
2017
Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio thiocyanoxidans ARh 2T
brenda
Tikhonova, T.V.; Sorokin, D.Y.; Hagen, W.R.; Khrenova, M.G.; Muyzer, G.; Rakitina, T.V.; Shabalin, I.G.; Trofimov, A.A.; Tsallagov, S.I.; Popov, V.O.
Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase
Proc. Natl. Acad. Sci. USA
117
5280-5290
2020
Thioalkalivibrio paradoxus, Thioalkalivibrio paradoxus ARh1, Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio thiocyanoxidans ARh2, Thioalkalivibrio thiocyanoxidans ARh4
brenda
Khrenova, M.; Soloveva, A.; Varfolomeeva, L.; Tikhonova, T.; Popov, V.
The O to S substitution in urea brings inhibition activity against thiocyanate dehydrogenase
Mendeleev Commun.
31
373-375
2021
Thioalkalivibrio sp.
-
brenda
EXTERNAL LINKS (specific for EC-Number 1.8.2.7)
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Release 2023.1 (January 2023)
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