Information on EC 1.8.1.20 - 4,4'-dithiodibutanoate disulfide reductase

for references in articles please use BRENDA:EC1.8.1.20
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The expected taxonomic range for this enzyme is: Rhodococcus erythropolis

EC NUMBER
COMMENTARY hide
1.8.1.20
-
RECOMMENDED NAME
GeneOntology No.
4,4'-dithiodibutanoate disulfide reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 4-sulfanylbutanoate + NAD+ = 4,4'-disulfanediyldibutanoate + NADH + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4,4'-disulfanediyldibutanoate degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
4-sulfanylbutanoate:NAD+ oxidoreductase
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4,4'-disulfanediyldibutanoate + NADH + H+
4-mercaptobutyric acid + NAD+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4,4'-disulfanediyldibutanoate + NADH + H+
4-mercaptobutyric acid + NAD+
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal cofactors are required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
4,4'-disulfanediyldibutanoate
-
at pH 8.0 and 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
4,4'-disulfanediyldibutanoate
-
at pH 8.0 and 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.36
-
at pH 8.0 and 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
-
calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65 - 85
-
after heating the enzyme for 15 min at 85C, it retains about 48% of its original activity, however, a stable activity could be recorded at 65C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
-
Ni-NTA column chromatography, and Superdex 200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) pLysS cells
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expressed in Escherichia coli BL21(DE3)pLysS cells