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EC Tree
Word Map
1.7.1.B3
hypoplasia
dermal
x-linked
goltz
ectodermal
malformation
ocular
porcupine
mesoderm
blaschko
skew
x-chromosome
postzygotic
teeth
goltz-gorlin
multisystemic
herniation
diaphragmatic
x-inactivation
angioma
papillomas
hernia
wingless
cutis
nail
o-acyltransferase
aplasia
patchy
environmental protection
medicine
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
nitrofurazone reductase,
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NADPH-nitrofurazone reductase
-
-
nitro/flavin reductase
-
-
nitrofurazone reductase
-
non-luminescent-bacterial NfsA/Frp-type enzyme
-
-
oxygen-insensitive nitroreductase (NADPH)
-
-
-
NfsA
-
-
ambiguous
nitroreductase A
-
-
additional information
-
cf. EC 1.5.1.30
additional information
-
cf. EC 1.6.5.5
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an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
an aromatic amine + NADP+ + H2O = an aromatic hydroxylamine + NADPH + H+
(1a)
-
-
an aromatic hydroxylamine + NADP+ = an aromatic nitric oxide + NADPH + H+
(1b)
-
-
an aromatic nitric oxide + NADP+ + H2O = an aromatic nitrate + NADPH + H+
(1c)
-
-
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
(overall reaction)
-
-
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
in vivo the reaction is obseverved in the opposite direction from that shown in the equation
-
-
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
catalytic mechanism analysis, two (four)-electron reduction of nitrobenzenes and single-step (H-) hydride transfer mechanism. NfsA follows a ping-pong mechanism, overview
-
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
the enzyme catalysis obeys the ping-pong Bi-Bi kinetic mechanism with substrate FMN as well as substrate nitrofurazone, but upon coupling with the bioluminescent reaction of luciferase, it changes to the sequential mechanism
-
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
the enzyme uses NADPH as the hydrogen donor to catalyze the reduction of a nitro group by a ping-pong Bi-Bi kinetic mechanism
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
the enzyme catalysis obeys the ping-pong Bi-Bi kinetic mechanism with substrate FMN as well as substrate nitrofurazone, but upon coupling with the bioluminescent reaction of luciferase, it changes to the sequential mechanism
-
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aromatic nitrate:NADPH oxidroreductase
-
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2,4,6-trinitrotoluene + NADPH + H+
4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O
2,4,6-trinitrotoluene + NADPH + H+
? + NADP+ + H2O
2,4-dinitrotoluene + NADPH + H+
? + NADP+ + H2O
-
-
?
2-amino-4,6-dinitrotoluene + NADPH + H+
? + NADP+ + H2O
-
-
?
4-amino-2,6-dinitrotoluene + NADPH + H+
? + NADP+ + H2O
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
? + NADP+
m-dinitrobenzene + NADPH + H+
? + NADP+
-
-
-
?
methyl 4-nitrobenzoate + NADPH + H+
? + NADP+ + H2O
-
-
-
?
nifuroxime + NADPH + H+
? + NADP+
-
-
-
?
nitro-CBI-5-[(dimethylamino)ethoxy]indole + NAD(P)H + H+
? + NAD(P)+
a nitro-CBI prodrug, low activity
-
?
nitrobenzene + NADPH + H+
? + NADP+
-
-
-
?
nitrofurantoin + NADPH + H+
? + NADP+
-
-
-
?
nitrofurazone + NADPH + H+
5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O
o-dinitrobenzene + NADPH + H+
? + NADP+
-
-
-
?
p-dinitrobenzene + NADPH + H+
? + NADP+
-
-
-
?
p-nitroacetophenone + NADPH + H+
? + NADP+
-
-
-
?
p-nitrobenzaldehyde + NADPH + H+
? + NADP+
-
-
-
?
p-nitrobenzoic acid + NADPH + H+
? + NADP+
-
-
-
?
tetryl + NADPH + H+
? + NADP+
-
-
-
?
additional information
?
-
2,4,6-trinitrotoluene + NADPH + H+
4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O
-
-
?
2,4,6-trinitrotoluene + NADPH + H+
4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O
i.e. TNT, NfsA reduces TNT to the para isomer 4-hydroxylamino-2,6-dinitrotoluene more easily than the ortho isomer 2-hydroxylamino-4,6-dinitrotoluene
-
?
2,4,6-trinitrotoluene + NADPH + H+
? + NADP+ + H2O
-
-
-
?
2,4,6-trinitrotoluene + NADPH + H+
? + NADP+ + H2O
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
? + NADP+
-
i.e. CB1954, an aziridinyl dinitrobenzamide prodrug
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
? + NADP+
i.e. CB1954, an aziridinyl dinitrobenzamide prodrug
-
?
nitrofurazone + NADPH + H+
5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O
-
-
-
?
nitrofurazone + NADPH + H+
5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O
-
-
?
additional information
?
-
-
the enzyme reduces both nitrofurazone and FMN effectively, see for EC 1.5.1.38, it is bifunctional as flavin reductase and nitroreductase. Two different FMN molecules are involved in the FMN reduction process: FMN tightly associated to the enzyme as prosthetic group and FMN as a substrate. The enzyme is also active with FAD, riboflavin, and lumiflavin, the two latter give the highest activity
-
?
additional information
?
-
an intrinsically oxygen-insensitive nature of the two-electron reduction mechanism
-
?
additional information
?
-
-
NADPH-dependent reduction of quinones, cf. EC 1.6.5.5, and nitroaromatic compounds by NfsA, overview. The reactivity of nitroaromatic compounds (log kcat/Km) follows a linear dependence on their single-electron reduction potential, indicating a limited role for compound structure or active site flexibility in their reactivity. The reactivity of quinones is lower than that of nitroaromatics having similar single-electron reduction potential values, except for the significantly enhanced reactivity of 2-OH-1,4-naphthoquinones. The reduction of quinones by NfsA is most consistent with a single-step (H-) hydride transfer mechanism, quantitative analysis of two-electron reduction of quinones and nitroaromatics, overview
-
?
additional information
?
-
NfsA has a broad substrate spectrum. Various nitroaromatic substrates, including 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954), 2,4,6-trinitrotoluene (TNT), and 2,4-dinitrotoluene (2,4-DNT), can serve as substrates
-
?
additional information
?
-
no activity with 2,4-diamino-6-nitrotoluene and 2,6-diamino-4-nitrotoluene
-
?
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2,4,6-trinitrotoluene + NADPH + H+
4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O
-
-
-
?
nitrofurazone + NADPH + H+
5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O
-
-
-
-
?
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additional information
-
NADPH is much more effective than NADH
-
FMN
a flavoenzyme which contains FMN as the prosthetic group
FMN
-
prosthetic group involved in the reaction
FMN
-
tightly associated to, two different FMN molecules are involved in the FMN reduction process: FMN tightly associated to the enzyme as prosthetic group and FMN as a substrate
NADPH
-
-
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dicoumarol
-
inhibits Escherichia coli enzyme NfsB far more strongly than enzyme NfsA, by acting as a competitive inhibitor towards NADPH, uncompetitive towards substrate tetryl
NADP+
-
inhibition of NfsA-catalyzed tetryl reduction by NADP+
additional information
the enzyme is O2-insensitive
-
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0.013 - 0.033
2,4,6-trinitrotoluene
0.176 - 0.366
2,4-dinitrotoluene
0.3257
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
0.6427
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
0.23 - 0.487
5-(aziridin-1-yl)-2,4-dinitrobenzamide
0.046
m-dinitrobenzene
-
pH 7.0, 25°C
0.031
nifuroxime
-
pH 7.0, 25°C
1.5
nitrobenzene
-
pH 7.0, 25°C
0.019
Nitrofurantoin
-
pH 7.0, 25°C
0.012 - 0.064
nitrofurazone
0.018
o-dinitrobenzene
-
pH 7.0, 25°C
0.037
p-dinitrobenzene
-
pH 7.0, 25°C
0.39
p-nitroacetophenone
-
pH 7.0, 25°C
0.17
p-nitrobenzaldehyde
-
pH 7.0, 25°C
2.5
p-nitrobenzoic acid
-
pH 7.0, 25°C
0.011
tetryl
-
pH 7.0, 25°C
additional information
additional information
-
0.013
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
0.02
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
0.029
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
0.029
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
0.033
2,4,6-trinitrotoluene
-
pH 7.0, 25°C
0.176
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
0.206
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
0.313
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
0.366
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
0.23
5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
pH 7.0, 25°C
0.322
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
0.327
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
0.332
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
0.487
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
0.00085
NADPH
-
pH 7.0, 23°C, recombinant enzyme, with nitrofurazone
0.049
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
0.054
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
0.085
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
0.085
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
0.012
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
0.0163
nitrofurazone
-
pH 7.0, 23°C, recombinant enzyme
0.022
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
0.023
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
0.064
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
-
enzyme kinetic analysis, overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics of recombinant His6-tagged enzyme
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics. For NfsA the oxidative half-reaction (i.e., the reoxidation of FMNH by the oxidant substrate) is a rate-limiting step, because the values of kcat at infinite concentrations of tetryl or 2,4,6-trinitrotoluene are substantially lower than the lowest rate of the reductive half reaction (the reduction of FMN by NADPH) measured in the preliminary rapid reaction experiments. Stopped-flow and single-turnover measurements
-
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0.67 - 89
2,4,6-trinitrotoluene
0.7 - 24.94
2,4-dinitrotoluene
12.36
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
1.05
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
1.08 - 36.64
5-(aziridin-1-yl)-2,4-dinitrobenzamide
55
m-dinitrobenzene
-
pH 7.0, 25°C
180
nifuroxime
-
pH 7.0, 25°C
14
nitrobenzene
-
pH 7.0, 25°C
136
Nitrofurantoin
-
pH 7.0, 25°C
0.8 - 19.37
nitrofurazone
60
o-dinitrobenzene
-
pH 7.0, 25°C
27
p-dinitrobenzene
-
pH 7.0, 25°C
59
p-nitroacetophenone
-
pH 7.0, 25°C
30
p-nitrobenzaldehyde
-
pH 7.0, 25°C
64
p-nitrobenzoic acid
-
pH 7.0, 25°C
0.67
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
4.95
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
27.06
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
56.2
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
56.21
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
89
2,4,6-trinitrotoluene
-
pH 7.0, 25°C
0.7
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
3.64
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
17.31
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
24.94
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
1.08
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
2.99
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
15
5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
pH 7.0, 25°C
24.27
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
36.64
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
0.46
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
0.78
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
9.01
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
16.3
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
0.8
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
1.82
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
15.73
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
19.37
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
50 - 2697
2,4,6-trinitrotoluene
2 - 142
2,4-dinitrotoluene
38
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
1.6
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
2 - 112
5-(aziridin-1-yl)-2,4-dinitrobenzamide
1195.7
m-dinitrobenzene
-
pH 7.0, 25°C
5806.5
nifuroxime
-
pH 7.0, 25°C
9.3
nitrobenzene
-
pH 7.0, 25°C
7157.9
Nitrofurantoin
-
pH 7.0, 25°C
3333.3
o-dinitrobenzene
-
pH 7.0, 25°C
729.7
p-dinitrobenzene
-
pH 7.0, 25°C
151.3
p-nitroacetophenone
-
pH 7.0, 25°C
176.5
p-nitrobenzaldehyde
-
pH 7.0, 25°C
25.6
p-nitrobenzoic acid
-
pH 7.0, 25°C
7727.27
tetryl
-
pH 7.0, 25°C
50
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
250
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
1350
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
1931
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged enzyme
1940
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
2697
2,4,6-trinitrotoluene
-
pH 7.0, 25°C
2
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
12
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
84
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
142
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
2
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
9
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
65.2
5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
pH 7.0, 25°C
75
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
112
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
5
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
16
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
167
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
191.7
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
10
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
80
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
720
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
1610
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
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0.018
dicoumarol
-
pH 7.0, 25°C
additional information
additional information
-
inhibition kinetics
-
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0.1
purified recombinant mutant F46A, pH 7.0, 22°C, substrate nitrofurazone
0.29
purified recombinant mutant F46N, pH 7.0, 22°C, substrate nitrofurazone
3.74
purified recombinant mutant F46Y, pH 7.0, 22°C, substrate nitrofurazone
52.5
-
purified recombinant untagged enzyme, pH 7.0, 23°C
7.75
purified recombinant wild-type enzyme, pH 7.0, 22°C, substrate nitrofurazone
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7
-
assay at
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22
assay at room temperature
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-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
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evolution
-
analysis of evolutionary or molecular mechanism of divergence of the nitroreductase/flavin reductase family, overview. The enzyme is similar to NfsA from Escherichia coli, overview
evolution
nitroreductases are a family of flavoproteins that catalyze the NAD(P)H-dependent reduction of nitro groups to hydroxylamino and/or amino groups on various nitro-substituted compounds
evolution
-
analysis of evolutionary or molecular mechanism of divergence of the nitroreductase/flavin reductase family, overview. The enzyme is similar to NfsA from Escherichia coli, overview
physiological function
-
NfsA has potential applications in the biodegradation of nitroaromatic environment pollutants, e.g. explosives, and is also of interest for the anticancer strategy gene-directed enzyme prodrug therapy
physiological function
residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA, Phe42 is important for maintaining NfsA activity and structure
additional information
-
comparisons of the quantitative structure-activity relationships of single-electron reduction of quinones and nitroaromatic compounds by dehydrogenase (electron transferase) flavoenzymes, overview
additional information
the enzyme crystal structure of NfsA, PDB ID 1F5V, is used for molecular ligand docking studies
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NFSA_ECOLI
Escherichia coli (strain K12)
240
0
26801
Swiss-Prot
-
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53000
-
recombinant untagged enzyme, gel filtration
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homodimer
-
2 * 28320, sequence calculation, 2 * 28000, recombinant untagged enzyme, SDS-PAGE
homodimer
-
2 * 28320, sequence calculation, 2 * 28000, recombinant untagged enzyme, SDS-PAGE
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F42A
site-directed mutagenesis, the mutant enzymes shows 99% reduced activity toward nitrofurazone compared to the wild-type, and loss of helix content
F42N
site-directed mutagenesis, the mutant enzymes shows 96% reduced activity toward nitrofurazone compared to the wild-type, and loss of helix content
F42Y
site-directed mutagenesis, the mutant enzymes shows 52% reduced activity toward nitrofurazone compared to the wild-type
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the enzyme is O2-insensitive
742167
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recombinant enzyme 23fold from Escherichia coli to homogeneity by dialysis, anion exchange chromatography, dialysis, two steps of Blue Sepharose affinity chromatography, and again dialysis
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% purity
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gene ipa-43d, DNA and amino acid sequence determination and analysis, genetic structure and sequence comparison, recombinant overexpression in Escherichia coli J83
-
gene nfsA, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene nfsA, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene nfsA, recombinant overexpression in Escherichia coli reporter strain SOS-R2, which contains a lacZ reporter gene under control of the SOS responsive promoter sfiA, functional nitroreductase NfsA expression in human HCT-116 cells
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environmental protection
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NfsA has potential applications in the biodegradation of nitroaromatic environment pollutants, e.g. explosives
medicine
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NfsA has potential applications in the anticancer strategy gene-directed enzyme prodrug therapy
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Valiauga, B.; Williams, E.M.; Ackerley, D.F.; Cenas, N.
Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA) Characterization of kinetics and substrate specificity
Arch. Biochem. Biophys.
614
14-22
2017
Escherichia coli
brenda
Zenno, S.; Kobori, T.; Tanokura, M.; Saigo, K.
Purification and characetrizatio of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase
Biosci. Biotechnol. Biochem.
62
1978-1987
1998
Bacillus subtilis, Bacillus subtilis ISW 1214
brenda
Yang, J.; Zhan, J.; Bai, J.; Liu, P.; Xue, Y.; Yang, Q.
Residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA
Biotechnol. Lett.
35
1693-1700
2013
Escherichia coli (P17117)
brenda
Green, L.K.; Syddall, S.P.; Carlin, K.M.; Bell, G.D.; Guise, C.P.; Mowday, A.M.; Hay, M.P.; Smaill, J.B.; Patterson, A.V.; Ackerley, D.F.
Pseudomonas aeruginosa NfsB and nitro-CBI-DEI - a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy
Mol. Cancer
12
58
2013
Escherichia coli (P17117)
brenda
Bai, J.; Yang, J.; Liu, P.; Yang, Q.
Transformation pathway of 2,4,6-trinitrotoluene by Escherichia coli nitroreductases and improvement of activity using structure-based mutagenesis
Process Biochem.
50
705-711
2015
Escherichia coli (P17117)
brenda
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1.7.1.B3 aromatic nitroreductase [NADPH]
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