Information on EC 1.5.98.3 - coenzyme F420:methanophenazine dehydrogenase

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The expected taxonomic range for this enzyme is: Methanosarcinales

EC NUMBER
COMMENTARY hide
1.5.98.3
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RECOMMENDED NAME
GeneOntology No.
coenzyme F420:methanophenazine dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
reduced coenzyme F420 + methanophenazine = oxidized coenzyme F420 + dihydromethanophenazine
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme B/coenzyme M regeneration I (methanophenazine-dependent)
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SYSTEMATIC NAME
IUBMB Comments
reduced coenzyme F420:methanophenazine oxidoreductase
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oxidized coenzyme F420 + reduced 2,3-dimethyl-1,4-naphthoquinone
reduced coenzyme F420 + oxidized 2,3-dimethyl-1,4-naphthoquinone
show the reaction diagram
oxidized coenzyme F420 + reduced 2-bromophenazine
reduced coenzyme F420 + oxidized 2-bromophenazine
show the reaction diagram
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
show the reaction diagram
oxidized coenzyme F420 + reduced ferredoxin
reduced coenzyme F420 + oxidized ferredoxin
show the reaction diagram
oxidized coenzyme F420 + reduced methyl viologen
reduced coenzyme F420 + oxidized methyl viologen
show the reaction diagram
oxidized coenzyme F420 + reduced phenazine
reduced coenzyme F420 + oxidized phenazine
show the reaction diagram
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?
oxidized coenzyme F420 + reduced tetramethyl-4-benzoquinone
reduced coenzyme F420 + oxidized tetramethyl-4-benzoquinone
show the reaction diagram
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-
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?
reduced coenzyme F420 + 2-bromophenazine
?
show the reaction diagram
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?
reduced coenzyme F420 + 2-hydroxyphenazine
?
show the reaction diagram
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?
reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
show the reaction diagram
reduced coenzyme F420 + methyl viologen
?
show the reaction diagram
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?
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
show the reaction diagram
reduced coenzyme F420 + oxidized metronidazole
oxidized coenzyme F420 + reduced metronidazole
show the reaction diagram
reduced coenzyme F420 + phenazine
?
show the reaction diagram
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-
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?
reduced coenzyme F420 + phenazine-1-carboxylic acid
?
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iron-sulfur center
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the enzyme contains iron-sulfur clusters
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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16 mol non-heme iron per mol enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
2-hydroxyphenazine
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at pH 7.0 and 25C
0.25
phenazine
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at pH 7.0 and 25C
0.0054
reduced coenzyme F420
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at pH 6.8 and 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25.5
reduced coenzyme F420
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at pH 6.8 and 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.6
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with reduced tetramethyl-4-benzoquinone as cosubstrate, at pH 7.0 and 37C
1.1
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with reduced 2,3-dimethyl-1,4-naphthoquinone as cosubstrate, at pH 7.0 and 37C; with reduced methyl viologen as cosubstrate, at pH 7.0 and 37C; with reduced phenazine as cosubstrate, at pH 7.0 and 37C
1.7
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with reduced 2-bromophenazine as cosubstrate, at pH 7.0 and 37C
1.8
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with phenazine as cosubstrate, at pH 7.0 and 25C
4.6
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with phenazine-1-carboxylic acid as cosubstrate, at pH 7.0 and 25C
8.4
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with 2-bromophenazine as cosubstrate, at pH 7.0 and 25C
8.8
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with 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 25C
10
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with methyl viologen as cosubstrate, at pH 7.0 and 25C
10.4
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with reduced 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
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isoelectric focusing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
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gel filtration
120000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the purified enzyme becomes rapidly inactivated by repeated freezing and thawing
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography
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