Any feedback?
Information on EC 1.5.3.7 - L-pipecolate oxidase
for references in articles please use BRENDA:EC1.5.3.7Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.5.3.7 L-pipecolate oxidaseIUBMB Comments
The product reacts with water to form (S)-2-amino-6-oxohexanoate.
Specify your search results
Word Map
- 1.5.3.7
- sarcosine
- saccharopine
- medicine
-
poly2-oxazoline
-
alpha-aminoadipic
- sardh
- zellweger
- n-methyltryptophan
The enzyme appears in viruses and cellular organisms
Synonyms
pipox, l-pipecolate oxidase, pipecolate oxidase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
L-pipecolate oxidase
L-pipecolic acid oxidase
-
-
-
-
pipecolate oxidase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
L-pipecolate:oxygen 1,6-oxidoreductase
The product reacts with water to form (S)-2-amino-6-oxohexanoate.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
81669-65-0
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-pipecolate + O2
(S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
?
L-pipecolate + O2
(S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
pipecolate metabolism leads to protection against H2O2 stress in mammalian cells. The enzyme is necessary for pipecolate protection
-
-
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
the pro-6(R)-hydrogen of pipecolic acid is removed stereospecifically
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
-
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
-
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
-
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
highly specific for
-
-
?
additional information
?
-
-
enzyme is involved in L-lysine metabolism
-
-
?
additional information
?
-
-
no activity with fructosyl-amino acids, L-saccharopine, and sarcosine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-pipecolate + O2
(S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
pipecolate metabolism leads to protection against H2O2 stress in mammalian cells. The enzyme is necessary for pipecolate protection
-
-
?
additional information
?
-
-
enzyme is involved in L-lysine metabolism
-
-
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
L-pipecolate + O2
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
the product reacts with water to form 2-aminoadipate 6-semialdehyde
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(S)-1,3-thiazane-4-carboxylic acid
-
causes time-dependent, irreversible, stereospecific inactivation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
di-(2-ethylhexyl)phthalate
-
5 g per kg body weight, 500% activity enhancement in the peroxisomal fraction
glucagon
-
3 mg per kg body weight, 150% activity enhancement in the mitochondrial fraction
additional information
-
activity is maximal when FAD, phenazine ethosulfate and glycerol are included in the assay
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Breast Neoplasms
Human snoRNA-93 is processed into a microRNA-like RNA that promotes breast cancer cell invasion.
Neoplasm Metastasis
Expression of sarcosine metabolism-related proteins according to metastatic site in breast cancer.
Peroxisomal Disorders
Assay for L-pipecolate oxidase activity in human liver: detection of enzyme deficiency in hyperpipecolic acidaemia.
Zellweger Syndrome
Assay for L-pipecolate oxidase activity in human liver: detection of enzyme deficiency in hyperpipecolic acidaemia.
Zellweger Syndrome
Identification of L-pipecolate oxidase in human liver and its deficiency in the Zellweger syndrome.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
36
(S)-1,3-thiazane-4-carboxylic acid
-
competitive inhibitor that completely inactivates the enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
white matter contains a more than 2-fold higher activity than cortex
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
knockdown of the enzyme by small interference RNA abolishes pipecolate protection against hydrogen peroxide-induced cell death in HEK293 cells suggesting a critical role for this enzyme
physiological function
pipecolate metabolism leads to protection against H2O2 stress in mammalian cells. The enzyme is necessary for pipecolate protection
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SOX_BOVIN
392
0
43820
Swiss-Prot
other Location (Reliability: 2)
SOX_HUMAN
390
1
44066
Swiss-Prot
other Location (Reliability: 2)
SOX_MOUSE
390
0
43847
Swiss-Prot
other Location (Reliability: 2)
SOX_RABIT
390
1
44122
Swiss-Prot
other Location (Reliability: 2)
Y9130_DICDI
1080
0
120674
Swiss-Prot
other Location (Reliability: 2)
SWNR_ARTBC
Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371)
305
0
34471
Swiss-Prot
other Location (Reliability: 2)
SWNR_METRA
Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075)
305
0
34289
Swiss-Prot
other Location (Reliability: 2)
FAP1_SCHPO
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
412
1
46320
Swiss-Prot
Secretory Pathway (Reliability: 3)
A0A5B7AAM8_DAVIN
433
0
46376
TrEMBL
other Location (Reliability: 5)
A0A1Z5JET4_FISSO
409
0
45481
TrEMBL
other Location (Reliability: 3)
A0A481V2Q7_9CAUD
115
0
11854
TrEMBL
Mitochondrion (Reliability: 4)
A0A3B0SI08_9ZZZZ
422
0
44388
TrEMBL
other Location (Reliability: 3)
W1QG18_OGAPD
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)
428
0
48045
TrEMBL
Secretory Pathway (Reliability: 4)
A0A2I0A787_9ASPA
423
0
45143
TrEMBL
other Location (Reliability: 4)
A0A2I0A793_9ASPA
443
0
48030
TrEMBL
other Location (Reliability: 3)
A0A654KF14_TAYEM
Taylorella equigenitalis (strain MCE9)
431
0
47474
TrEMBL
-
Q88CC4_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
432
0
47023
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
-
10 min 20% activity, bovine serum albumin at 1 mg per ml protects from thermal inactivation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several weeks, 100% activity
-
4°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several days, 100% activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
-
expressed as a chimera between maltose-binding protein and L-pipecolic acid oxidase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
medicine
-
L-pipecolic acid is formed by the catabolism of lysine in humans, and its accumulation is one of the first biochemical abnormalities detected in the Zellweger syndrome
medicine
-
L-pipecolic oxidase activity is deficient in patients with peroxisome biogenesis disorders
medicine
-
L-pipecolic oxidase activity is deficient in patients with peroxisome biogenesis disorders
medicine
-
L-pipecolic oxidase activity is deficient in patients with peroxisome biogenesis disorders
medicine
-
the L-pipecolate pathway for degradation of lysine is the principal one in the brain
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Naranjo, L.; Martin de Valmaseda, E.; Banuelos, O.; Lopez, P.; Riano, J.; Casqueiro, J.; Martin, J.F.
Conversion of pipecolic acid into lysine in Penicillium chrysogenum requires pipecolate oxidase and saccharopine reductase: characterization of the lys7 gene encoding saccharopine reductase
J. Bacteriol.
183
7165-7172
2001
Penicillium chrysogenum
brenda
Dodt, G.; Kim, D.; Reimann, S.; McCabe, K.; Gould, S.J.; Mihalik, S.J.
The human L-pipecolic acid oxidase is similar to bacterial monomeric sarcosine oxidases rather than D-amino acid oxidases
Cell Biochem. Biophys.
32
313-316
2000
Homo sapiens
brenda
Ijlst, L.; de Kromme, I.; Oostheim, W.; Wanders, R.J.A.
Molecular cloning and expression of human L-pipecolate oxidase
Biochem. Biophys. Res. Commun.
270
1101-1105
2000
Homo sapiens
brenda
Zabriskie, T.M.; Liang, X.
Mechanism-based inactivity of L-pipecolate oxidase by a sulfur-containing substrate analog, 5-thia-L-pipecolic acid
Bioorg. Med. Chem. Lett.
7
457-462
1997
Macaca mulatta
-
brenda
Zabriskie, T.M.; Kelly, W.L.; Liang, X.
Stereochemical course of the oxidation of L-pipecolic acid by the flavoenzyme L-pipecolate oxidase
J. Am. Chem. Soc.
119
6446-6447
1997
Macaca mulatta
-
brenda
Rao, V.V.; Tsai, M.J.; Pan, X.; Chang, Y.F.
L-Pipecolic acid oxidation in rat: subcellular localization and developmental study
Biochim. Biophys. Acta
1164
29-35
1993
Rattus norvegicus
brenda
Rao, V.V.; Chang, Y.F.
L-Pipecolic acid metabolism in human liver: detection of L-pipecolate oxidase and identification of its reaction product
Biochim. Biophys. Acta
1038
295-299
1990
Homo sapiens
brenda
Wanders, R.J.A.; Romeyn, G.J.; Schutgens, R.B.H.; Tager, J.M.
L-pipecolate oxidase: a distinct peroxisomal enzyme in man
Biochem. Biophys. Res. Commun.
164
550-555
1989
Homo sapiens
brenda
Mihalik, S.J.; Rhead, W.J.
L-Pipecolic acid oxidation in the rabbit and cynomolgus monkey. Evidence for differing organellar locations and cofactor requirements in each species
J. Biol. Chem.
264
2509-2517
1989
Oryctolagus cuniculus, Macacine alphaherpesvirus 1
brenda
Kinzel, J.J.; Bhattacharjee, J.K.
Lysine biosynthesis in Rhodotorula glutinis: properties of pipecolic acid oxidase
J. Bacteriol.
151
1073-1077
1982
Rhodotorula glutinis
brenda
Yoshida, N.; Akazawa, S.I.; Katsuragi, T.; Tani, Y.
Characterization of two fructosyl-amino acid oxidase homologs of Schizosaccharomyces pombe
J. Biosci. Bioeng.
97
278-280
2004
Schizosaccharomyces pombe
brenda
Natarajan, S.K.; Muthukrishnan, E.; Khalimonchuk, O.; Mott, J.L.; Becker, D.F.
Evidence for pipecolate oxidase in mediating protection against hydrogen peroxide stress
J. Cell. Biochem.
118
1678-1688
2017
Homo sapiens, Homo sapiens (Q9P0Z9)
brenda
Posset, R.; Opp, S.; Struys, E.A.; Voelkl, A.; Mohr, H.; Hoffmann, G.F.; Koelker, S.; Sauer, S.W.; Okun, J.G.
Understanding cerebral L-lysine metabolism the role of L-pipecolate metabolism in Gcdh-deficient mice as a model for glutaric aciduria type I
J. Inherit. Metab. Dis.
38
265-272
2015
Mus musculus
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 1.5.3.7)