Information on EC 1.5.1.19 - D-nopaline dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

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EC NUMBER
COMMENTARY hide
1.5.1.19
-
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RECOMMENDED NAME
GeneOntology No.
D-nopaline dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
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SYSTEMATIC NAME
IUBMB Comments
N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming)
In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
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CAS REGISTRY NUMBER
COMMENTARY hide
64763-57-1
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Agrobacterium tumefaciens C58 / ATCC 33970
-
UniProt
Manually annotated by BRENDA team
inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTi37
-
-
Manually annotated by BRENDA team
sunflower, crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
show the reaction diagram
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
show the reaction diagram
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
show the reaction diagram
-
in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
-
?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
show the reaction diagram
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
show the reaction diagram
-
in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
-
substrate inhibition above 10fold Km
NADPH
-
substrate inhibition above 10fold Km
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
2-oxoglutarate
-
-
0.74
L-arginine
-
-
3.7
L-ornithine
-
-
0.008 - 1
NADPH
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
nopaline synthesis
6.5
-
ornaline synthesis
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
induced by Agrobacterium tumefaciens, strain C58 or T37 in Helianthus annuus L.
Manually annotated by BRENDA team
-
axenic tumor tissue and shoots regenerated from tumor tissue of Brassica juncea inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTiT37
Manually annotated by BRENDA team
additional information
-
no activity in habituated tissue or in octopine-type crown gall tissue
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
4 * 40000, SDS-PAGE
158000
-
crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37, gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 40000, SDS-PAGE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4�C, several months in 10 mM Tris
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Nicotiana tabacum NT1 cells under nos-promoter with internal deletions
-
expression of Agrobacterium tumor-inducing plasmid pTiT37 gene encoding nopaline synthase in Escherichia coli
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gene nos, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
internal deletions in the nos-promoter
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LINKS TO OTHER DATABASES (specific for EC-Number 1.5.1.19)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)