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EC Tree
IUBMB Comments In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
nopaline synthase, nopaline dehydrogenase,
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D-nopaline synthase
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dehydrogenase, nopaline
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flavin-containing opine dehydrogenase
nopaline dehydrogenase
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Atu6015
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flavin-containing opine dehydrogenase
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flavin-containing opine dehydrogenase
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nopaline synthase
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NOS
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N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
ter-bi rapid-equilibrium random-order mechanism
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N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
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N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming)
In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
additional information
?
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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?
L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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i.e. nopaline
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
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?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
the reduction reaction is highly preferred
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?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
the reduction reaction is highly preferred
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?
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
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i.e. ornaline
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L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
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?
additional information
?
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analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+
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?
additional information
?
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analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+
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?
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
-
?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
-
in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
-
?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
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NADPH
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2-oxoglutarate
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substrate inhibition above 10fold Km
NADPH
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substrate inhibition above 10fold Km
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0.008
NADPH
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nopaline synthesis
0.009
NADPH
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ornaline synthesis
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UniProt
brenda
inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTi37
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brenda
sunflower, crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
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brenda
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brenda
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UniProt
brenda
expression in Nicotiana tabacum NT1 cells
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brenda
expression of Agrobacterium tumor-inducing plasmid pTiT37 encoding nopaline synthase gene in Escherichia coli
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brenda
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induced by Agrobacterium tumefaciens, strain C58 or T37 in Helianthus annuus L.
brenda
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axenic tumor tissue and shoots regenerated from tumor tissue of Brassica juncea inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTiT37
brenda
additional information
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no activity in habituated tissue or in octopine-type crown gall tissue
brenda
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DHNO_AGRT7
413
0
45387
Swiss-Prot
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DHNO_AGRVI
412
0
45279
Swiss-Prot
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A0A1S7S7T4_9RHIZ
412
0
45303
TrEMBL
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A0A1S7NGQ8_9RHIZ
170
0
17899
TrEMBL
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A3NGV8_BURP6
Burkholderia pseudomallei (strain 668)
430
0
44340
TrEMBL
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Q59158_RHIRD
434
0
46162
TrEMBL
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Q59157_RHIRD
371
1
39979
TrEMBL
-
A3S0A4_RALSL
466
0
49376
TrEMBL
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A8EIQ9_BURPE
430
0
44298
TrEMBL
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A2QFT2_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
384
0
41808
TrEMBL
other Location (Reliability: 4 )
A0A1S7NHB7_9RHIZ
242
0
27407
TrEMBL
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A0A0E1S0W0_BURPE
430
0
44342
TrEMBL
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A0A0E1W223_BURPE
430
0
44298
TrEMBL
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B1HBZ5_BURPE
430
0
44372
TrEMBL
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A3P2F9_BURP0
Burkholderia pseudomallei (strain 1106a)
430
0
44372
TrEMBL
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A9CL08_AGRFC
Agrobacterium fabrum (strain C58 / ATCC 33970)
413
0
45434
TrEMBL
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158000
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crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37, gel filtration
40000
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4 * 40000, SDS-PAGE
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tetramer
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4 * 40000, SDS-PAGE
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additional information
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internal deletions in the nos-promoter
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4°C, several months in 10 mM Tris
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crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
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recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration
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expression in Nicotiana tabacum NT1 cells under nos-promoter with internal deletions
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expression of Agrobacterium tumor-inducing plasmid pTiT37 gene encoding nopaline synthase in Escherichia coli
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gene nos, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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Kemp, J.D.; Sutton, D.W.; Hack, E.
Purification and characterization of the crown gall specific enzyme nopaline synthase
Biochemistry
18
3755-3760
1979
Helianthus annuus
brenda
Gafni, Y.; Chilton, M.D.
Expression of the nopaline synthase gene in Escherichia coli
Gene
39
141-146
1985
Agrobacterium tumefaciens
brenda
Mathews, V.H.; Bhatia, C.R.; Mitra, R.; Krishna, T.G.; Rao, P.S.
Regeneration of shoots from Brassica juncea (Linn) czern and coss cells transformed by agrobacterium tumefaciens and expression of nopaline dehydrogenase genes
Plant Sci.
39
49-54
1985
Brassica juncea
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brenda
Kim, Y.; An, G.
Characterization of a hexamer motif and b element of the nopaline synthase (nos) promoter
Mol. Cells
7
84-89
1997
Agrobacterium tumefaciens
brenda
Monneuse, M.O.; Rouze, P.
Sequence comparisons between Agrobacterium tumefaciens T-DNA-encoded octopine and nopaline dehydrogenases and other nucleotide-requiring enzymes: structural and evolutionary implications
J. Mol. Evol.
25
46-57
1987
Agrobacterium tumefaciens
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brenda
Watanabe, S.; Sueda, R.; Fukumori, F.; Watanabe, Y.
Characterization of flavin-containing opine dehydrogenase from bacteria
PLoS ONE
10
e0138434
2015
Agrobacterium tumefaciens (A9CL08), Agrobacterium tumefaciens C58 / ATCC 33970 (A9CL08)
brenda
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1.5.1.19 D-nopaline dehydrogenase
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