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EC Tree
IUBMB Comments In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
Atu6015 , D-nopaline synthase, dehydrogenase, nopaline, flavin-containing opine dehydrogenase, nopaline dehydrogenase, nopaline synthase,
NOS ,
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D-nopaline synthase
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dehydrogenase, nopaline
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flavin-containing opine dehydrogenase
nopaline dehydrogenase
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Atu6015
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flavin-containing opine dehydrogenase
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flavin-containing opine dehydrogenase
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nopaline synthase
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NOS
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N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
ter-bi rapid-equilibrium random-order mechanism
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N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
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N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming)
In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
additional information
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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i.e. nopaline
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
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L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
the reduction reaction is highly preferred
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L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
the reduction reaction is highly preferred
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L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
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i.e. ornaline
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L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
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additional information
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analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+
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additional information
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analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+
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L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
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?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
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in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
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?
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
L-arginine + 2-oxoglutarate + NADPH + H+
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
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ir
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NADPH
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2-oxoglutarate
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substrate inhibition above 10fold Km
NADPH
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substrate inhibition above 10fold Km
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Hepatitis
Agroinoculation of the cloned infectious cDNAs of Lettuce chlorosis virus results in systemic plant infection and production of whitefly transmissible virions.
Hepatitis
Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones.
Hepatitis
Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector.
Infections
A disarmed binary vector from Agrobacterium tumefaciens functions in Agrobacterium rhizogenes : Frequent co-transformation of two distinct T-DNAs.
Infections
Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector.
Infertility
Transgenic sterility in Populus: expression properties of the poplar PTLF, Agrobacterium NOS and two minimal 35S promoters in vegetative tissues.
Neoplasms
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
Neoplasms
Detection of bacterial chitinase activity in transformed plant tumour cells using a specific exochitinase substrate.
Neoplasms
Differential expression of crown gall tumor markers in transformants obtained after in vitro Agrobacterium tumefaciens-induced transformation of cell wall regenerating protoplasts derived from Nicotiana tabacum.
Neoplasms
The expression of a nopaline synthase - human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissue.
Neoplasms
The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants.
Neoplasms
The role of cytosine methylation in the control of nopaline synthase gene expression in a plant tumor.
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0.008
NADPH
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nopaline synthesis
0.009
NADPH
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ornaline synthesis
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UniProt
brenda
inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTi37
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brenda
sunflower, crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
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brenda
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brenda
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UniProt
brenda
expression in Nicotiana tabacum NT1 cells
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brenda
expression of Agrobacterium tumor-inducing plasmid pTiT37 encoding nopaline synthase gene in Escherichia coli
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brenda
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induced by Agrobacterium tumefaciens, strain C58 or T37 in Helianthus annuus L.
brenda
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axenic tumor tissue and shoots regenerated from tumor tissue of Brassica juncea inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTiT37
brenda
additional information
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no activity in habituated tissue or in octopine-type crown gall tissue
brenda
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DHNO_AGRT7
413
0
45387
Swiss-Prot
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DHNO_AGRVI
412
0
45279
Swiss-Prot
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Q59157_RHIRD
371
1
39979
TrEMBL
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A3NGV8_BURP6
Burkholderia pseudomallei (strain 668)
430
0
44340
TrEMBL
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A0A1S7NHB7_9RHIZ
242
0
27407
TrEMBL
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A0A1S7NGQ8_9RHIZ
170
0
17899
TrEMBL
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A8EIQ9_BURPE
430
0
44298
TrEMBL
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A3S0A4_RALSL
466
0
49376
TrEMBL
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Q59158_RHIRD
434
0
46162
TrEMBL
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A0A1S7S7T4_9RHIZ
412
0
45303
TrEMBL
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A2QFT2_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
384
0
41808
TrEMBL
other Location (Reliability: 4 )
A3P2F9_BURP0
Burkholderia pseudomallei (strain 1106a)
430
0
44372
TrEMBL
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A0A0E1S0W0_BURPE
430
0
44342
TrEMBL
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A0A0E1W223_BURPE
430
0
44298
TrEMBL
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B1HBZ5_BURPE
430
0
44372
TrEMBL
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A9CL08_AGRFC
Agrobacterium fabrum (strain C58 / ATCC 33970)
413
0
45434
TrEMBL
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158000
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crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37, gel filtration
40000
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4 * 40000, SDS-PAGE
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tetramer
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4 * 40000, SDS-PAGE
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additional information
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internal deletions in the nos-promoter
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4°C, several months in 10 mM Tris
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crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
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recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration
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expression in Nicotiana tabacum NT1 cells under nos-promoter with internal deletions
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expression of Agrobacterium tumor-inducing plasmid pTiT37 gene encoding nopaline synthase in Escherichia coli
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gene nos, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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Kemp, J.D.; Sutton, D.W.; Hack, E.
Purification and characterization of the crown gall specific enzyme nopaline synthase
Biochemistry
18
3755-3760
1979
Helianthus annuus
brenda
Gafni, Y.; Chilton, M.D.
Expression of the nopaline synthase gene in Escherichia coli
Gene
39
141-146
1985
Agrobacterium tumefaciens
brenda
Mathews, V.H.; Bhatia, C.R.; Mitra, R.; Krishna, T.G.; Rao, P.S.
Regeneration of shoots from Brassica juncea (Linn) czern and coss cells transformed by agrobacterium tumefaciens and expression of nopaline dehydrogenase genes
Plant Sci.
39
49-54
1985
Brassica juncea
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brenda
Kim, Y.; An, G.
Characterization of a hexamer motif and b element of the nopaline synthase (nos) promoter
Mol. Cells
7
84-89
1997
Agrobacterium tumefaciens
brenda
Monneuse, M.O.; Rouze, P.
Sequence comparisons between Agrobacterium tumefaciens T-DNA-encoded octopine and nopaline dehydrogenases and other nucleotide-requiring enzymes: structural and evolutionary implications
J. Mol. Evol.
25
46-57
1987
Agrobacterium tumefaciens
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brenda
Watanabe, S.; Sueda, R.; Fukumori, F.; Watanabe, Y.
Characterization of flavin-containing opine dehydrogenase from bacteria
PLoS ONE
10
e0138434
2015
Agrobacterium tumefaciens (A9CL08), Agrobacterium tumefaciens C58 / ATCC 33970 (A9CL08)
brenda
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1.5.1.19 D-nopaline dehydrogenase
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