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EC Tree
The enzyme appears in viruses and cellular organisms
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GOGAT
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2 L-glutamate + NAD(P)+ = L-glutamine + 2-oxoglutarate + NAD(P)H + H+
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L-glutamate:NAD(P)+ oxidoreductase (transaminating)
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2 L-glutamate + NAD+
L-glutamine + 2-oxoglutarate + NADH + H+
2 L-glutamate + NADP+
L-glutamine + 2-oxoglutarate + NADPH + H+
2 L-glutamate + NAD+
L-glutamine + 2-oxoglutarate + NADH + H+
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r
2 L-glutamate + NAD+
L-glutamine + 2-oxoglutarate + NADH + H+
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r
2 L-glutamate + NADP+
L-glutamine + 2-oxoglutarate + NADPH + H+
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r
2 L-glutamate + NADP+
L-glutamine + 2-oxoglutarate + NADPH + H+
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r
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2 L-glutamate + NAD+
L-glutamine + 2-oxoglutarate + NADH + H+
2 L-glutamate + NADP+
L-glutamine + 2-oxoglutarate + NADPH + H+
2 L-glutamate + NAD+
L-glutamine + 2-oxoglutarate + NADH + H+
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r
2 L-glutamate + NAD+
L-glutamine + 2-oxoglutarate + NADH + H+
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r
2 L-glutamate + NADP+
L-glutamine + 2-oxoglutarate + NADPH + H+
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r
2 L-glutamate + NADP+
L-glutamine + 2-oxoglutarate + NADPH + H+
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r
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NAD+
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NADH
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NADP+
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NADPH
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azaserine
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strong inhibition
azaserine
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strong inhibition
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0.6
2-oxoglutarate
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pH 7.5, 30°C, L3 stage enzyme
0.8
2-oxoglutarate
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pH 7.5, 30°C, adult worm enzyme
0.9
2-oxoglutarate
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pH 7.5, 30°C
0.6
L-glutamine
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pH 7.5, 30°C, L3 stage enzyme
1
L-glutamine
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pH 7.5, 30°C
1.5
L-glutamine
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pH 7.5, 30°C, adult worm enzyme
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0.024 - 0.041
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pH 7.5, 30°C, L3 stage
0.033 - 0.059
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pH 7.5, 30°C, adult worm
0.051 - 0.095
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pH 7.5, 30°C, adult worm
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7.5
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30
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assay at
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brenda
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brenda
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L3 stage
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additional information
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no activity in L3 stage
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Umair, S.; Bland, R.J.; Simpson, H.V.
Glutamate synthase, but not GABA shunt enzymes, contributes to nitrogen metabolism of the sheep abomasal nematode parasites Haemonchus contortus and Teladorsagia circumcincta
Exp. Parasitol.
127
9-13
2011
Haemonchus contortus, Teladorsagia circumcincta
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1.4.1.B5 glutamate synthase (NAD(P)H)
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