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Information on EC - glutamate synthase (NADPH)

for references in articles please use BRENDA:EC1.4.1.13
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IUBMB Comments
Binds FMN, FAD, 2 [4Fe-4S] clusters and 1 [3Fe-4S] cluster. The reaction takes place in the direction of L-glutamate production. The protein is composed of two subunits, alpha and beta. The alpha subunit is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC, glutamate dehydrogenase [NADP+]). The beta subunit transfers electrons to the cosubstrate. The NH3 is channeled through a 31 A channel in the active protein. In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced. In the intact alphabeta complex, ammonia production only takes place as part of the overall reaction.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
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EC, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), more
2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+
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L-glutamate + NADP+ + H2O = NH3 + 2-oxoglutarate + NADPH + H+
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L-glutamate + NH3 = L-glutamine + H2O
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