Information on EC 1.3.7.13 - 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.7.13
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RECOMMENDED NAME
GeneOntology No.
3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster = 3,8-divinyl protochlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bacteriochlorophyll a biosynthesis
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bacteriochlorophyll c biosynthesis
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bacteriochlorophyll d biosynthesis
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bacteriochlorophyll e biosynthesis
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chlorophyll a biosynthesis III
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Porphyrin and chlorophyll metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
protochlorophyllide-a:ferredoxin C-81-oxidoreductase
The enzyme, found in many phototrophic bacteria, land plants, and some green and red algae, is involved in the production of monovinyl versions of (bacterio)chlorophyll pigments from their divinyl precursors. Binds two [4Fe-4S] clusters and an FAD cofactor. It can also act on 3,8-divinyl chlorophyllide a, 3,8-divinyl chlorophyll a, and chlorophyll c2. cf. EC 1.3.1.75, 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH).
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
show the reaction diagram
preferred substrate
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?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
show the reaction diagram
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
show the reaction diagram
8-vinyl protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
show the reaction diagram
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?
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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-
-
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?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
show the reaction diagram
P74473
preferred substrate
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?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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-
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
show the reaction diagram
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
show the reaction diagram
8-vinyl protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
show the reaction diagram
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?
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ferredoxin [iron-sulfur] cluster
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NADPH
[4Fe-4S] cluster
recombinant BciB binds two [4Fe-4S] clusters, one of which may not be essential for the activity of the enzyme, determined by EPR spectroscopy as well as quantitative analyses of bound iron and sulfide
[4Fe-4S]-center
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the enzyme binds two [4Fe-4S] clusters, one of which may not be essential for the activity of the enzyme
additional information
BciB requires an additional electron donor, ferredoxin, together with NADPH
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at room temperature
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
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x * 45000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene bciB, recombinant C-terminally His6-tagged from Escherichia coli strain AHS-1 under anaerobic conditions by cobalt affinity chromatography, ultrafiltration, and desalting gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803
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expressed in Escherichia coli
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gene bciB or frhB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, frhB is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
B0CAB4;
gene bciB, cloning in Escherichia coli strain JM109, recombinant expression of C-terminally His6-tagged in Escherichia coli strain BL21(DE3). Plasmid pET28-CthaBciB is cotransformed into strain BL21(DE3) along with the plasmid pDB1282, which contains the genes iscS, iscU, iscA, hscA, hscB, and fdx for Fe/S-cluster assembly from Azotobacter vinelandii under the control of an arabinose-inducible promoter to produce Escherichia coli strain AHS-1
gene slr1923, phylogenetic analysis
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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generation of an enzyme BciB knockout mutant and recombinant expression of Rhodobacter sphaeroides gene bciA in the mutant