Information on EC 1.3.1.B13 - polyprenol reductase (NADPH)

for references in articles please use BRENDA:EC1.3.1.B13
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.B13
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
polyprenol reductase (NADPH)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a dolichol + NADP+ = a polyprenol + NADPH + H+
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
dolichol:NADP+ alpha-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
no activity in Escherichia coli
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a dolichol + NADP+
a polyprenol + NADPH + H+
show the reaction diagram
reduction of the alpha-isoprene subunit of polyprenol
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-
?
a polyprenol + NADPH + H+
a dolichol + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a polyprenol + NADPH + H+
a dolichol + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in HEK293T cells
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recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of isozyme PPRD1 in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis; recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of wild-type an dmutant isozyme PPRD1s in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H321L
site-directed mutagenesis, the mutant shows 47% of wild-type reductase activity
H336L
site-directed mutagenesis, the mutant shows 45% of wild-type reductase activity
H321L
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site-directed mutagenesis, the mutant shows 47% of wild-type reductase activity
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H336L
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site-directed mutagenesis, the mutant shows 45% of wild-type reductase activity
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H296G
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inactive mutant enzyme, shows no reduction of polyprenol
additional information