Information on EC 1.3.1.86 - crotonyl-CoA reductase

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bcd2

butanoyl-CoA:(acceptor) 2,3-oxidoreductase

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butyryl coenzyme A dehydrogenase

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ambiguous

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butyryl dehydrogenase

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butyryl-CoA dehydrogenase

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ambiguous

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butyryl-CoA dehydrogenase complex

CCR

4 entries

CD1054

crotonyl CoA reductase

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712233

crotonyl coenzyme A reductase

crotonyl-CoA reductase

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crotonyl-coenzyme A reductase

3 entries

enoyl-coenzyme A reductase

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ambiguous

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ethylene reductase

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ambiguous

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short-chain acyl CoA dehydrogenase

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ambiguous

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short-chain acyl-coenzyme A dehydrogenase

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ambiguous

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trans-crotonyl CoA reductase

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Biosynthesis of secondary metabolites, Butanoate metabolism, Microbial metabolism in diverse environments

unsaturated acyl coenzyme A reductase

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ambiguous

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unsaturated acyl-CoA reductase

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butanoyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + NADPH + H+

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carboxylation

Salinispora tropica

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reductive carboxylation

712235

KEGG

butanoyl-CoA:NADP+ 2,3-oxidoreductase

Catalyses the reaction in the reverse direction. This enzyme from Streptomyces collinus is specific for (E)-but-2-enoyl-CoA, and is proposed to provide butanoyl-CoA as a starter unit for straight-chain fatty acid biosynthesis.

37251-07-3

cf. EC 1.3.1.8

(E)-but-2-enoyl-CoA + NADPH + H+

butanoyl-CoA + NADP+

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711366, 712641

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?

crotonyl-CoA + NADH + ferredoxin

butyryl-CoA + ?

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-

?

crotonyl-CoA + NADPH + H+

butanoyl-CoA + NADP+

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the enzyme exhibits a high substrate specificity for crotonyl-CoA

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?

crotonyl-CoA + NADPH + H+

butyryl-CoA + NADP+

5 entries

trans-crotonyl-CoA + NADPH + H+

butyryl-CoA + NADP+

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the crotonyl-CoA reductase reaction requires NADPH as electron donor

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?

additional information

?

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FAD

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in the absence of ferredoxin, a remarkable stimulation of crotonyl-CoA reduction by FAD is observed

Ferredoxin

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butyryl-CoA dehydrogenase from C. difficile belongs to the subfamily of bifurcating enzymes capable of coupling the exergonic reduction of crotonyl-CoA by NAD Hwith the endergonic reduction of ferredoxin by NADH

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NADH

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NADPH

5 entries

2-mercaptoethanol

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at high concentrations beta-mercaptoethanol is inhibitory

2-methylcrotonyl-CoA

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CoA-activated enzyme shows competitive inhibition with the substrate analog 2-methylcrotonyl-CoA

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole

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extremely potent inhibitor

5,5'-dithiobis(2-nitrobenzoic acid)

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extremely potent inhibitor

acetyl-CoA

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competitive inhibitor

ammonium sulfate

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enzyme activity is inhibited by ammonium sulfate ; however, this inhibition is overcome by addition of 10 mM guanidine

arachidoyl-CoA

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86% residual activity at 0.1 mM

butyryl-CoA

Ca2+

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complete inhibition at 1 mM

Co2+

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complete inhibition at 1 mM

dithiothreitol

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at high concentrations dithiothreitol is inhibitory

iodoacetamide

iodoacetate

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isomyristoyl-CoA

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78% residual activity at 0.1 mM

isopalmitoyl-CoA

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95% residual activity at 0.1 mM

malonyl-CoA

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noncompetitive inhibitor

Mg2+

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30% inhibition at 1 mM

Mn2+

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complete inhibition at 1 mM

myristoyl-CoA

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36% residual activity at 0.1 mM

N-ethylmaleimide

NADP+

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NADPH

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concentrations of NADPH above 0.2 mM lead to inhibition of enzyme activity

p-chloromercuribenzoate

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a 30-min incubation of crotonyl-CoA reductase with p-chloromercuribenzoate at 0.008 mM leads to approximately 8.5% inhibition of enzyme activity

palmitoyl-CoA

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24% residual activity at 0.1 mM

stearoyl-CoA

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92% residual activity at 0.1 mM

Zn2+

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55% inhibition at 1 mM

additional information

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no significant inhibition of the activity of crotonyl-CoA reductase is observed upon addition of either flavin adenine dinucleotide (0.018 and 0.072 mM) or flavin mononucleotide (0.013 and 0.130 mM)

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CoA

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free CoA is a strong activator of the reductase reaction

L-arabinose

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about 2fold CCR activity is obtained by the addition of 0.1% (w/v) of L-arabinose

711366

additional information

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no significant activation of the activity of crotonyl-CoA reductase is observed upon addition of either flavin adenine dinucleotide (0.018 and 0.072 mM) or flavin mononucleotide (0.013 and 0.130 mM)

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0.01

butyryl-CoA

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pH 7.5, temperature not specified in the publication

0.0025 - 0.018

crotonyl-CoA

0.002

Ferredoxin

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pH 7.5, temperature not specified in the publication

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0.145

NADH

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pH 7.5, temperature not specified in the publication

0.00364 - 0.015

NADPH

0.0033

trans-crotonyl-CoA

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in 200 mM potassium phosphate buffer, pH 6.8, at 26Â°C

2 - 8

butyryl-CoA

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pH 7.5, temperature not specified in the publication

19

crotonyl-CoA

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pH 7.5, temperature not specified in the publication

37

Ferredoxin

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pH 7.5, temperature not specified in the publication

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3 - 6

NADH

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pH 7.5, temperature not specified in the publication

2800

butyryl-CoA

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pH 7.5, temperature not specified in the publication

7600

crotonyl-CoA

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pH 7.5, temperature not specified in the publication

18500

Ferredoxin

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pH 7.5, temperature not specified in the publication

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250

NADH

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pH 7.5, temperature not specified in the publication

0.0029

2-methylcrotonyl-CoA

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pH and temperature not specified in the publication

0.006

acetyl-CoA

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pH and temperature not specified in the publication

0.005 - 0.9

butyryl-CoA

0.5

isomyristoyl-CoA

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in 50 mM Tris/HCI pH 6.5 and 10% (v/v) glycerol, at 30Â°C

0.4

isopalmitoyl-CoA

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Ki above 0.4 mM, in 50 mM Tris/HCI pH 6.5 and 10% (v/v) glycerol, at 30Â°C

0.021

malonyl-CoA

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pH and temperature not specified in the publication

0.017

myristoyl-CoA

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in 50 mM Tris/HCI pH 6.5 and 10% (v/v) glycerol, at 30Â°C

0.63

NADP+

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in 50 mM Tris/HCI pH 6.5 and 10% (v/v) glycerol, at 30Â°C

0.0095

palmitoyl-CoA

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in 50 mM Tris/HCI pH 6.5 and 10% (v/v) glycerol, at 30Â°C

0.0008

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native enzyme, pH and temperature not specified in the publication

713435

0.00091

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native enzyme from crude extract, pH 7.5 at 30Â°C

0.0061

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crude extract, pH 6.8, at 26Â°C

0.0156

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recombinant enzyme, pH and temperature not specified in the publication

713435

0.0501

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after 11.2fold purification, pH 6.8, at 26Â°C

0.333

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recombinant enzyme from crude extract, pH 7.5 at 30Â°C

2.889

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native enzyme after 3068fold purification, pH 7.5 at 30Â°C

3.316

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recombinant enzyme after 10fold purification, pH 7.5 at 30Â°C

6.5

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7 - 8.5

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7.5

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assay at

37

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40

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390850, 390852, 711170

-

-

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Salinispora tropica

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712235

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Streptomyces hygroscopicus subsp. ascomyceticus

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656737, 711884, 712233, 712270, 713435

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712617

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culture condition:acetate-grown cell

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712270

mammary gland

Streptomyces hygroscopicus subsp. ascomyceticus

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390850, 390852, 711170

mycelium

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712617

physiological function

7 entries

malfunction

3 entries

metabolism

4 entries

110000

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48000

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2 * 48000, SDS-PAGE

49400

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2 * 49400, calculated from amino acid sequence

85000

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native protein, gel filtration

homodimer

40

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at 40Â°C the enzyme retains 47% of its activity after 30 min

the crotonyl-CoA reductase activity is influenced by preincubation. 0.25 M Tris-HCl buffer diminishes the crotonyl-CoA reductase activity by approximately 30%

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ammonium sulfate precipitation, calcium phosphate gel treatment, alumina gel Cgamma extract filtration, Sephadex G-200 gel filtration

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ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose column chromatography, Mono Q column chromatography, Sephadex G-100 gel filtration, and phenyl-Superose gel filtration

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expressed in Escherichia coli

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expressed in Escherichia coli BL21 (DE3)/pZYB3 cells

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expressed in Ralstonia eutropha strain PHB-4

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711366

expressed in Streptomyces cinnamonensis strain L1

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656737

expressed in Streptomyces erythraea strain EAT4

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713435

the addition of 1.0 g/l L-lysine induces expression of the ccr gene in Streptomyces cinnamonensis strain C730.1 in chemically defined medium containing mainly D-glucose (12 g/l), L-tyrosine (3.3 g/l), and either L-valine (6.7 g/l) or L-leucine (7.5 g/l) as carbon and nitrogen sources