Information on EC 1.3.1.71 - DELTA24(241)-sterol reductase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.3.1.71
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RECOMMENDED NAME
GeneOntology No.
DELTA24(241)-sterol reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ergosterol + NADP+ = ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ergosterol biosynthesis I
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Steroid biosynthesis
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Metabolic pathways
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
ergosterol:NADP+ DELTA24(241)-oxidoreductase
Acts on a range of steroids with a 24(241)-double bond.
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CAS REGISTRY NUMBER
COMMENTARY hide
56467-82-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
dimorphic fungus
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
show the reaction diagram
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
show the reaction diagram
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
show the reaction diagram
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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about 10fold less active than NADPH
NADPH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20-piperidin-2-yl-5alpha-pregnan-20(R),3beta-diol
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i.e. AZA-1, inhibits the enzyme and the DELTA24-sterol methyltransferase, leads to accumulation of ergosta-5,7,24(28)-trien-3beta-ol in vivo
22-piperidin-2-yl-5alpha-pregnan-3beta,22(S)-diol
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i.e. AZA-2, specific inhibition of the enzyme, leads to accumulatin of ergosta-5,7,22,24(28)-tetraen-3beta-ol in vivo
22-piperidin-3-yl-5alpha-pregnan-3beta,22(S)-diol
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i.e. AZA-3, inhibits the enzyme, major proliferative effect and accumulation of lanosterol in vivo
23-Azacholesterol
additional information
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sterol composition with or without addition of azasterol analogue inhibitors, overview
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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5fold increase in enzyme activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0108
ergosta-5,7,22,24(241)-tetraen-3beta-ol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00032
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enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes
0.74
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recombinant enzyme, at 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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organism in its pathogenic yeast-like phase
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
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x * 56000, deduced from immunoblot
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 56000, deduced from immunoblot
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
25% glycerol and cofactor NADPH or NADH at 1.3 mM stabilize the enzyme
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anaerobiosis leads to loss of 15% activity after 24 h
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, after 24 h: completely stable in presence of NADPH or NADH, 42% remaining activity in presence of 10 mM GSH or 2-mercaptoethanol, 33% remaining activity without additives, after 48 h: 93% remianing activity in prsence of 25% glycerol, 37% with 10% glycerol, and 13% remaining activity without additives
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
combined overexpression with sterol acyltransferase in Saccharomyces cerevisiae strain YEH56(pHXA42)
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green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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LINKS TO OTHER DATABASES (specific for EC-Number 1.3.1.71)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)