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Enzyme Nomenclature
Information on EC 1.3.1.71 - DELTA24(241)-sterol reductase
for references in articles please use BRENDA:EC1.3.1.71
Word Map on EC 1.3.1.71
- 1.3.1.71
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pneumocystis
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carinii
- lanosterol
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opportunistic
- zymosterol
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human-derived
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jiroveci
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lamin
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aids-associated
- azasterol
- fenpropimorph
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beta-ol
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immunodeficient
- pombe
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protozoan
- tridemorph
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schizosaccharomyces
- copper
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delta7
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
1.3.1.71
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RECOMMENDED NAME
GeneOntology No.
DELTA24(241)-sterol reductase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ergosterol + NADP+ = ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
acts on a range of steroids with a 24(241)-double bond
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ergosterol + NADP+ = ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
ergosterol:NADP+ DELTA24(241)-oxidoreductase
Acts on a range of steroids with a 24(241)-double bond.
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CAS REGISTRY NUMBER
COMMENTARY
56467-82-4
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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-
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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trivial name ergosterol
?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosterol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosterol + NADP+
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?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
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?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
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step in the biosynthesis of ergosta-5,22-dien-3bta-ol, i.e. brassicasterol, the major sterol compound in Paracoccidioides brasiliensis
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ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
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preferred substrate
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?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
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terminal step in ergosterol biosynthesis, pathway overview, presursor analysis
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?
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
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?
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
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?
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
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?
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
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trivial name ergosterol
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?
additional information
?
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enzyme-deficient mutants accumulate ergosta-5,7,22,24(24 1)-tetraen-3beta-ol, ergosta-5,7,24(28)-trien-3beta-ol, ergosta-7,24(28)-dien-3beta-ol, stigmasta-5,7,22,24(28)-tetraenol, stigmasta-5,7,24(28)-trienol, stigmasta-8,24(28)-dienol, and stigmasta-7,24(28)-dienol, while ergosterol and 7-dehydroporiferasterol are absent, in contrary to the wild-type strain, overview
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additional information
?
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enzyme-deficient mutants accumulate ergosta-5,7,22,24(24 1)-tetraen-3beta-ol, ergosta-5,7,24(28)-trien-3beta-ol, ergosta-7,24(28)-dien-3beta-ol, stigmasta-5,7,22,24(28)-tetraenol, stigmasta-5,7,24(28)-trienol, stigmasta-8,24(28)-dienol, and stigmasta-7,24(28)-dienol, while ergosterol and 7-dehydroporiferasterol are absent, in contrary to the wild-type strain, overview
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additional information
?
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sterol composition with or without addition of azasterol analogue inhibitors, overview
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additional information
?
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the enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions whereas most fungal SAM:SMT transfer only one methyl group to the side chain
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additional information
?
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the enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions whereas most fungal SAM:SMT transfer only one methyl group to the side chain
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additional information
?
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ergosta-7,22,24(28)-trien-3beta-ol, episterol, and fecosterol are poor substrates, 24,25-dihydro-24-methylene lanosterol is no substrate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
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trivial name ergosterol
?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosterol + NADP+
A0A0W4ZCX9
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?
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosterol + NADP+
A0A0W4ZCX9
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?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
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step in the biosynthesis of ergosta-5,22-dien-3bta-ol, i.e. brassicasterol, the major sterol compound in Paracoccidioides brasiliensis
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-
?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
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terminal step in ergosterol biosynthesis, pathway overview, presursor analysis
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?
additional information
?
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enzyme-deficient mutants accumulate ergosta-5,7,22,24(24 1)-tetraen-3beta-ol, ergosta-5,7,24(28)-trien-3beta-ol, ergosta-7,24(28)-dien-3beta-ol, stigmasta-5,7,22,24(28)-tetraenol, stigmasta-5,7,24(28)-trienol, stigmasta-8,24(28)-dienol, and stigmasta-7,24(28)-dienol, while ergosterol and 7-dehydroporiferasterol are absent, in contrary to the wild-type strain, overview
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additional information
?
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enzyme-deficient mutants accumulate ergosta-5,7,22,24(24 1)-tetraen-3beta-ol, ergosta-5,7,24(28)-trien-3beta-ol, ergosta-7,24(28)-dien-3beta-ol, stigmasta-5,7,22,24(28)-tetraenol, stigmasta-5,7,24(28)-trienol, stigmasta-8,24(28)-dienol, and stigmasta-7,24(28)-dienol, while ergosterol and 7-dehydroporiferasterol are absent, in contrary to the wild-type strain, overview
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additional information
?
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sterol composition with or without addition of azasterol analogue inhibitors, overview
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
20-piperidin-2-yl-5alpha-pregnan-20(R),3beta-diol
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i.e. AZA-1, inhibits the enzyme and the DELTA24-sterol methyltransferase, leads to accumulation of ergosta-5,7,24(28)-trien-3beta-ol in vivo
22-piperidin-2-yl-5alpha-pregnan-3beta,22(S)-diol
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i.e. AZA-2, specific inhibition of the enzyme, leads to accumulatin of ergosta-5,7,22,24(28)-tetraen-3beta-ol in vivo
22-piperidin-3-yl-5alpha-pregnan-3beta,22(S)-diol
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i.e. AZA-3, inhibits the enzyme, major proliferative effect and accumulation of lanosterol in vivo
additional information
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sterol composition with or without addition of azasterol analogue inhibitors, overview
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00032
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enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
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organism in its pathogenic yeast-like phase
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
25% glycerol and cofactor NADPH or NADH at 1.3 mM stabilize the enzyme
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, after 24 h: completely stable in presence of NADPH or NADH, 42% remaining activity in presence of 10 mM GSH or 2-mercaptoethanol, 33% remaining activity without additives, after 48 h: 93% remianing activity in prsence of 25% glycerol, 37% with 10% glycerol, and 13% remaining activity without additives
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
combined overexpression with sterol acyltransferase in Saccharomyces cerevisiae strain YEH56(pHXA42)
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gene erg6, recombinant expression in transgenic Saccharomyces cerevisiae erg6 knockout mutant strain and functional complementation
green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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construction of 3 enzyme-deficient strains resistant to nystatin, analysis of sterol content and spetra in the mutants, overview
additional information
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construction of 3 enzyme-deficient strains resistant to nystatin, analysis of sterol content and spetra in the mutants, overview
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additional information
analysis of sterol compositions of wild-type Sacchromyces cerevisiae strain BY4742, the erg6 knockout mutant (DELTAerg6), and DELTAerg6 expressing the Pneumocystis carinii or the Saccharomyces cerevisiae erg6 gene by a variety of chromatographic and spectroscopic procedures. The Pneumocystis carinii SAM:SMT in the DELTAerg6 restores its ability to produce the C28 sterol ergosterol as the major sterol, and also results in low levels of C29 sterols, overview. While the Pneumocystis carinii SAM:SMT in the yeast DELTAerg6 cells is able to transfer a second methyl group to the side chain, the action of DELTA24(28)-sterol reductase (coded by the erg4 gene) in the yeast cells prevents the formation and accumulation of as many C29 sterols as that found in Pneumocystis carinii
additional information
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analysis of sterol compositions of wild-type Sacchromyces cerevisiae strain BY4742, the erg6 knockout mutant (DELTAerg6), and DELTAerg6 expressing the Pneumocystis carinii or the Saccharomyces cerevisiae erg6 gene by a variety of chromatographic and spectroscopic procedures. The Pneumocystis carinii SAM:SMT in the DELTAerg6 restores its ability to produce the C28 sterol ergosterol as the major sterol, and also results in low levels of C29 sterols, overview. While the Pneumocystis carinii SAM:SMT in the yeast DELTAerg6 cells is able to transfer a second methyl group to the side chain, the action of DELTA24(28)-sterol reductase (coded by the erg4 gene) in the yeast cells prevents the formation and accumulation of as many C29 sterols as that found in Pneumocystis carinii
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