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Information on EC 1.3.1.54 - precorrin-6A reductase
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The expected taxonomic range for this enzyme is: Archaea, Bacteria
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EC NUMBER 
COMMENTARY 
1.3.1.54
-
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RECOMMENDED NAME
GeneOntology No.
precorrin-6A reductase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
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-
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precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
reaction mechanism
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precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
reaction mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
reduction
oxidation
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-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
precorrin-6B:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
137573-72-9
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137672-84-5
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Methanococcus maripaludis cbiJ and surrounding sequence
SwissProt
Methanococcus maripaludis cbiJ and surrounding sequence
SwissProt
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
precorrin-6A + NADPH
precorrin-6B + NADP+
-
-
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
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enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
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-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
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step in the biosynthesis of cobalamine, vitamin B12
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-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
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stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
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-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
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stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
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-
?
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
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-
-
-
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
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reduction step along the pathway to corrins
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-
-
additional information
?
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enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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-
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additional information
?
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
-
reduction step along the pathway to corrins
-
-
-
precorrin-6A + NADPH
precorrin-6B + NADP+
-
enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
additional information
?
-
Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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additional information
?
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Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
26000
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x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
28095
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x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
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x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant soluble enzyme from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis of gene cobK, subcloning of gene cobK for mutant construction in Escherichia coli strain S17-1
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
JJ117-12
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-13
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-14
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-15
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ120
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ121
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ122
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ201
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ202
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ240
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ241
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ242
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ243
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-12
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ117-13
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ117-14
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ117-15
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ120
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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additional information
additional information
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
additional information
-
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
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LINKS TO OTHER DATABASES (specific for EC-Number 1.3.1.54)
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