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Information on EC 1.3.1.54 - precorrin-6A reductase for references in articles please use BRENDA:EC1.3.1.54
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EC Tree
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms
precorrin-6x reductase, precorrin 6-x reductase,
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precorrin-6 reductase
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precorrin-6Y:NADP+ oxidoreductase
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Reductase, precorrin 6x (Pseudomonas denitrificans clone pXL367 gene cobK)
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precorrin 6-X reductase
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precorrin 6-X reductase
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precorrin-6A reductase
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precorrin-6A reductase
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precorrin-6x reductase
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precorrin-6x reductase
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precorrin-6x reductase
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precorrin-6x reductase
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precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
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precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
reaction mechanism
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precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
reaction mechanism
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oxidation
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reduction
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precorrin-6B:NADP+ oxidoreductase
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precorrin-6A + NADPH
precorrin-6B + NADP+
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
additional information
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precorrin-6A + NADPH
precorrin-6B + NADP+
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precorrin-6A + NADPH
precorrin-6B + NADP+
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enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
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precorrin-6A + NADPH
precorrin-6B + NADP+
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step in the biosynthesis of cobalamine, vitamin B12
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precorrin-6A + NADPH
precorrin-6B + NADP+
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stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
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precorrin-6A + NADPH
precorrin-6B + NADP+
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step in the biosynthesis of cobalamine, vitamin B12
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precorrin-6A + NADPH
precorrin-6B + NADP+
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stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
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Precorrin-6X + NADPH
Precorrin-6Y + NADP+
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Precorrin-6X + NADPH
Precorrin-6Y + NADP+
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reduction step along the pathway to corrins
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additional information
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enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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additional information
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enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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precorrin-6A + NADPH
precorrin-6B + NADP+
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
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reduction step along the pathway to corrins
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additional information
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precorrin-6A + NADPH
precorrin-6B + NADP+
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enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
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precorrin-6A + NADPH
precorrin-6B + NADP+
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step in the biosynthesis of cobalamine, vitamin B12
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precorrin-6A + NADPH
precorrin-6B + NADP+
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step in the biosynthesis of cobalamine, vitamin B12
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additional information
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Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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additional information
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Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
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Methanococcus maripaludis cbiJ and surrounding sequence
SwissProt
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Methanococcus maripaludis cbiJ and surrounding sequence
SwissProt
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SwissProt
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SwissProt
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brenda
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brenda
recombinant strain
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brenda
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Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
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26000
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x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
28080
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calculation from nucleotide sequence
28095
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x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
31000
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1 * 31000, SDS-PAGE
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x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
monomer
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1 * 31000, SDS-PAGE
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JJ117-12
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-13
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-14
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-15
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ120
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ121
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ122
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ201
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ202
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ240
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ241
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ242
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ243
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-12
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ117-13
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ117-14
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ117-15
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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JJ120
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
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additional information
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
additional information
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cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
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recombinant soluble enzyme from Escherichia coli
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DNA and amino acid sequence determination and analysis of gene cobK, subcloning of gene cobK for mutant construction in Escherichia coli strain S17-1
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3)
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Roessner, C.A.; Spencer, J.B.; Ozaki, S.; Min, C.; Atshaves, B.P.; Nayar, P.; Anousis, N.; Stolowich, N.J.; Holderman, M.T.; Scott, A.I.
Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes
Protein Expr. Purif.
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155-163
1995
Pseudomonas denitrificans (nomen rejiciendum)
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Blanche, F.; Thibaut, D.; Famechon, A.; Debussche, L.; Cameron, B.; Crouzet, J.
Precorrin-6x reductase from Pseudomonas denitrificans: purification and characterization of the enzyme and identification of the structural gene
J. Bacteriol.
174
1036-1042
1992
Pseudomonas denitrificans (nomen rejiciendum)
brenda
Shearer, N.; Hinsley, A.P.; Van Spanning, R.J.; Spiro, S.
Anaerobic growth of Paracoccus denitrificans requires cobalamin: characterization of cobK and cobJ genes
J. Bacteriol.
181
6907-6913
1999
Paracoccus denitrificans, Paracoccus denitrificans (Q9R9K2), Paracoccus denitrificans Pd 1222 (Q9R9K2)
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Kiuchi, F.; Thibaut, D.; Debussche, L.; Leeper, F.; Blanche, F.; Battersby, A.R.
Biosynthesis of vitamin B12: stereochemistry of transfer of a hydride equivalent from NADPH by precorrin-6x reductase
J. Chem. Soc.
Chem. Commun.
306-308
1992
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 Rif
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brenda
Kim, W.; Major, T.A.; Whitman, W.B.
Role of the precorrin 6-X reductase gene in cobamide biosynthesis in Methanococcus maripaludis
Archaea
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375-384
2005
Methanococcus maripaludis, Methanococcus maripaludis (Q977V1), Methanococcus maripaludis JJ (Q977V1)
brenda
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1.3.1.54 precorrin-6A reductase
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