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Information on EC 1.3.1.54 - precorrin-6A reductase
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1.3.1.54 precorrin-6A reductaseIUBMB Comments
The enzyme, which participates in the aerobic (late cobalt insertion) pathway of adenosylcobalamin biosynthesis, catalyses the reduction of the double bond between C-18 and C-19 of precorrin-6A. See EC 1.3.1.106, cobalt-precorrin-6A reductase, for the corresponding enzyme that participates in the anaerobic cobalamin biosynthesis pathway.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
precorrin 6-X reductase, precorrin-6 reductase, precorrin-6A reductase, precorrin-6x reductase, precorrin-6Y:NADP+ oxidoreductase, Reductase, precorrin 6x (Pseudomonas denitrificans clone pXL367 gene cobK), 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
precorrin 6-X reductase
precorrin-6A reductase
precorrin-6x reductase
precorrin-6Y:NADP+ oxidoreductase
-
-
-
-
Reductase, precorrin 6x (Pseudomonas denitrificans clone pXL367 gene cobK)
-
-
-
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precorrin-6x reductase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
-
-
-
-
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
reaction mechanism
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precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
reaction mechanism
Pseudomonas denitrificans (nom. rej.) SC510 Rif
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
reduction
oxidation
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
precorrin-6B:NADP+ oxidoreductase
The enzyme, which participates in the aerobic (late cobalt insertion) pathway of adenosylcobalamin biosynthesis, catalyses the reduction of the double bond between C-18 and C-19 of precorrin-6A. See EC 1.3.1.106, cobalt-precorrin-6A reductase, for the corresponding enzyme that participates in the anaerobic cobalamin biosynthesis pathway.
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CAS REGISTRY NUMBER
COMMENTARY
137573-72-9
-
137672-84-5
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
precorrin-6A + NADPH
precorrin-6B + NADP+
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enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
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-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
Pseudomonas denitrificans (nom. rej.) SC510 Rif
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
Pseudomonas denitrificans (nom. rej.) SC510 Rif
-
stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
-
-
?
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
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reduction step along the pathway to corrins
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-
?
additional information
?
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
?
additional information
?
-
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
?
additional information
?
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
-
reduction step along the pathway to corrins
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
Pseudomonas denitrificans (nom. rej.) SC510 Rif
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
additional information
?
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
?
additional information
?
-
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
?
additional information
?
-
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Methanococcus maripaludis cbiJ and surrounding sequence
SwissProt
brenda
Methanococcus maripaludis cbiJ and surrounding sequence
SwissProt
brenda
Show AA Sequence and Transmembrane Helices (2864 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
26000
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
28095
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
JJ117-12
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mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ243
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ242
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ241
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ240
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ202
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ201
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ122
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ121
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ120
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-12
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-15
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-14
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-13
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ120
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-12
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-13
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-14
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-15
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
additional information
additional information
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
additional information
-
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
additional information
-
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis of gene cobK, subcloning of gene cobK for mutant construction in Escherichia coli strain S17-1
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roessner, C.A.; Spencer, J.B.; Ozaki, S.; Min, C.; Atshaves, B.P.; Nayar, P.; Anousis, N.; Stolowich, N.J.; Holderman, M.T.; Scott, A.I.
Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes
Protein Expr. Purif.
6
155-163
1995
Pseudomonas denitrificans (nom. rej.)
brenda
Blanche, F.; Thibaut, D.; Famechon, A.; Debussche, L.; Cameron, B.; Crouzet, J.
Precorrin-6x reductase from Pseudomonas denitrificans: purification and characterization of the enzyme and identification of the structural gene
J. Bacteriol.
174
1036-1042
1992
Pseudomonas denitrificans (nom. rej.)
brenda
Shearer, N.; Hinsley, A.P.; Van Spanning, R.J.; Spiro, S.
Anaerobic growth of Paracoccus denitrificans requires cobalamin: characterization of cobK and cobJ genes
J. Bacteriol.
181
6907-6913
1999
Paracoccus denitrificans (Q9R9K2), Paracoccus denitrificans, Paracoccus denitrificans Pd 1222 (Q9R9K2)
brenda
Kiuchi, F.; Thibaut, D.; Debussche, L.; Leeper, F.; Blanche, F.; Battersby, A.R.
Biosynthesis of vitamin B12: stereochemistry of transfer of a hydride equivalent from NADPH by precorrin-6x reductase
J. Chem. Soc.
Chem. Commun.
306-308
1992
Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) SC510 Rif
-
brenda
Kim, W.; Major, T.A.; Whitman, W.B.
Role of the precorrin 6-X reductase gene in cobamide biosynthesis in Methanococcus maripaludis
Archaea
1
375-384
2005
Methanococcus maripaludis (Q977V1), Methanococcus maripaludis, Methanococcus maripaludis JJ (Q977V1)
brenda
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