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(E)-2-hexanoyl-CoA + NADPH
hexanoyl-CoA + NADP+
2-decenoyl-CoA + NADH + H+
decanoyl-CoA + NAD+
-
-
-
-
r
2-hexadecenoyl-CoA + NADH + H+
hexadecanoyl-CoA + NAD+
-
-
-
-
r
acyl-CoA + NAD+
trans-2,3-didehydroacyl-CoA + NADH
crotonyl-CoA + NAD(P)H
butyryl-CoA + NAD(P)+
-
-
-
?
crotonyl-CoA + NADH
butanoyl-CoA + NAD+
crotonyl-CoA + NADH
butyryl-CoA + NAD+
crotonyl-CoA + NADH + H+
butyryl-CoA + NAD+
crotonyl-CoA + NADPH
butanoyl-CoA + NADP+
crotonyl-CoA + NADPH + H+
butyryl-CoA + NADP+
dodec-2-enoyl-CoA + NADH + H+
dodecanoyl-CoA + NAD+
-
-
-
-
r
hexenoyl-CoA + NADH
? + NAD+
trans-2-decenoyl-CoA + NADH
decanoyl-CoA + NAD+
trans-2-dodecenoyl-CoA + NADH
dodecanoyl-CoA + NAD+
trans-2-dodecenoyl-CoA + NADH + H+
dodecanoyl-CoA + NAD+
trans-2-hexadecenoyl-CoA + NADH
hexadecanoyl-CoA + NAD+
trans-2-hexenoyl-CoA + NAD(P)H
hexanoyl-CoA + NAD(P)+
trans-2-hexenoyl-CoA + NADH + H+
hexanoyl-CoA + NAD+
-
-
-
?
trans-2-octenoyl-CoA + NADH
octanoyl-CoA + NAD+
trans-didehydroacyl-CoA + NAD(P)H
acyl-CoA + NAD(P)+
i.e. enoyl-CoA
-
-
?
additional information
?
-
(E)-2-hexanoyl-CoA + NADPH

hexanoyl-CoA + NADP+
-
-
-
?
(E)-2-hexanoyl-CoA + NADPH
hexanoyl-CoA + NADP+
-
-
-
?
acyl-CoA + NAD+

trans-2,3-didehydroacyl-CoA + NADH
-
contributes to fatty acid synthesis beta-oxidation in mitochondria
-
-
?
acyl-CoA + NAD+
trans-2,3-didehydroacyl-CoA + NADH
-
fatty acid elongation
-
-
?
crotonyl-CoA + NADH

butanoyl-CoA + NAD+
-
-
-
?, ir
crotonyl-CoA + NADH
butanoyl-CoA + NAD+
-
-
-
-
ir
crotonyl-CoA + NADH
butanoyl-CoA + NAD+
-
-
-
ir
crotonyl-CoA + NADH
butanoyl-CoA + NAD+
high activity toward crotonyl-CoA
-
-
?
crotonyl-CoA + NADH
butanoyl-CoA + NAD+
-
high activity toward crotonyl-CoA
-
-
?
crotonyl-CoA + NADH

butyryl-CoA + NAD+
-
negative cooperativity in the reaction with substrate
-
-
?
crotonyl-CoA + NADH
butyryl-CoA + NAD+
-
reductase I highest activity
-
-
?
crotonyl-CoA + NADH + H+

butyryl-CoA + NAD+
-
crotonyl-CoA is reduced to butyryl-CoA by NADH, but not by NADPH, only in the presence of flavin nucleotides (FMN or FAD)
-
-
?
crotonyl-CoA + NADH + H+
butyryl-CoA + NAD+
-
-
-
r
crotonyl-CoA + NADH + H+
butyryl-CoA + NAD+
-
-
-
-
?
crotonyl-CoA + NADH + H+
butyryl-CoA + NAD+
-
-
-
?
crotonyl-CoA + NADH + H+
butyryl-CoA + NAD+
-
-
-
?
crotonyl-CoA + NADPH

butanoyl-CoA + NADP+
-
-
-
?
crotonyl-CoA + NADPH
butanoyl-CoA + NADP+
-
-
-
?
crotonyl-CoA + NADPH
butanoyl-CoA + NADP+
-
-
-
?
crotonyl-CoA + NADPH + H+

butyryl-CoA + NADP+
NADPH is a poor cosubstrate
-
-
?
crotonyl-CoA + NADPH + H+
butyryl-CoA + NADP+
NADPH is a poor cosubstrate
-
-
?
hexenoyl-CoA + NADH

? + NAD+
weak activity toward crotonyl-CoA
-
-
?
hexenoyl-CoA + NADH
? + NAD+
-
weak activity toward crotonyl-CoA
-
-
?
trans-2-decenoyl-CoA + NADH

decanoyl-CoA + NAD+
-
reductase II: highest activity
-
-
?
trans-2-decenoyl-CoA + NADH
decanoyl-CoA + NAD+
-
-
-
-
?
trans-2-dodecenoyl-CoA + NADH

dodecanoyl-CoA + NAD+
-
-
-
-
?
trans-2-dodecenoyl-CoA + NADH
dodecanoyl-CoA + NAD+
-
-
i.e. laurate
?
trans-2-dodecenoyl-CoA + NADH + H+

dodecanoyl-CoA + NAD+
-
-
-
?
trans-2-dodecenoyl-CoA + NADH + H+
dodecanoyl-CoA + NAD+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADH

hexadecanoyl-CoA + NAD+
-
-
-
-
?
trans-2-hexadecenoyl-CoA + NADH
hexadecanoyl-CoA + NAD+
-
-
-
-
?
trans-2-hexenoyl-CoA + NAD(P)H

hexanoyl-CoA + NAD(P)+
-
-
-
?
trans-2-hexenoyl-CoA + NAD(P)H
hexanoyl-CoA + NAD(P)+
-
reductase III: highest activity, utilizes substrates of chain length C4-C12
-
-
?
trans-2-octenoyl-CoA + NADH

octanoyl-CoA + NAD+
-
reductase II: highest activity
-
-
?
trans-2-octenoyl-CoA + NADH
octanoyl-CoA + NAD+
-
-
-
-
?
trans-2-octenoyl-CoA + NADH
octanoyl-CoA + NAD+
-
-
-
-
?
additional information

?
-
no activity with butanoyl-CoA
-
-
?
additional information
?
-
-
no activity with butanoyl-CoA
-
-
?
additional information
?
-
-
reductase isoforms are chain length-specific
-
-
?
additional information
?
-
enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation
-
-
?
additional information
?
-
-
enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation
-
-
?
additional information
?
-
-
inert to substrates with chain length below C8
-
-
?
additional information
?
-
-
inert to substrates with chain length below C8
-
-
?
additional information
?
-
-
rat hepatic microsomal long-chain and short-chain trans-2-enoyl-CoA reductases with cofactor requirement (NADH or NADPH) depending on substrate chain length are characterized. Since no cis-2-enoyl-CoA compounds are tested as substrates a classification according to EC 1.3.1.8, EC 1.3.1.38 or EC 1.3.1.44 is impossible
-
-
?
additional information
?
-
-
long-chain substrates preferred
-
-
?
additional information
?
-
-
not active with NADPH or hexenoyl-CoA
-
-
?
additional information
?
-
-
no activity with butanoyl-CoA
-
-
?
additional information
?
-
no activity with butanoyl-CoA
-
-
?
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0.2327
(2E)-oct-2-enoyl-CoA
-
-
0.0893
2-decenoyl-CoA
-
-
0.004
2-dodecenoyl-CoA
pH and temperature not specified in the publication, mutant I287A
0.0027
crotonoyl-CoA
-
in potassium phosphate buffer pH 6.2, at 30°C
0.049 - 0.21
crotonyl-CoA
0.0558
dodec-2-enoyl-CoA
-
-
0.0295
hexadecenoyl-CoA
-
-
0.0034 - 0.038
hexanoyl-CoA
0.0011 - 0.004
trans-2-dodecenoyl-CoA
0.0034 - 0.091
trans-2-hexenoyl-CoA
0.109
Biochanin A

recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate
0.109
Biochanin A
cosubstrate crotonyl-CoA, pH 6.2, 30°C
0.049
crotonyl-CoA

mutant Y240F, pH not specified in the publication, temperature not specified in the publication
0.049
crotonyl-CoA
pH and temperature not specified in the publication, mutant Y240F
0.06
crotonyl-CoA
mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication
0.06
crotonyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A
0.068
crotonyl-CoA
pH 6.2, 30°C
0.068
crotonyl-CoA
recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor
0.07
crotonyl-CoA
wild-type, pH not specified in the publication, temperature not specified in the publication
0.07
crotonyl-CoA
pH and temperature not specified in the publication, wild-type
0.1
crotonyl-CoA
mutant F295A, pH not specified in the publication, temperature not specified in the publication
0.1
crotonyl-CoA
pH and temperature not specified in the publication, mutant F295A
0.11
crotonyl-CoA
mutant L291A, pH not specified in the publication, temperature not specified in the publication
0.11
crotonyl-CoA
pH and temperature not specified in the publication, mutant L291A
0.15
crotonyl-CoA
mutant Y370A, pH not specified in the publication, temperature not specified in the publication
0.15
crotonyl-CoA
pH and temperature not specified in the publication, mutant Y370A
0.16
crotonyl-CoA
mutant I287A, pH not specified in the publication, temperature not specified in the publication
0.16
crotonyl-CoA
pH and temperature not specified in the publication, mutant I287A
0.21
crotonyl-CoA
mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication
0.21
crotonyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A/F295A
0.0034
hexanoyl-CoA

pH and temperature not specified in the publication, mutant I287A
0.007
hexanoyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A
0.012
hexanoyl-CoA
pH and temperature not specified in the publication, wild-type
0.015
hexanoyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A/F295A
0.018
hexanoyl-CoA
pH and temperature not specified in the publication, mutant Y370A
0.019
hexanoyl-CoA
pH and temperature not specified in the publication, mutant F295A
0.038
hexanoyl-CoA
pH and temperature not specified in the publication, mutant L291A
0.0052
NADH

wild-type, pH not specified in the publication, temperature not specified in the publication
0.0316
NADH
pH 6.2, 25°C, mutant F11K
0.0316
NADH
mutant enzyme F11K, at pH 6.2 and 25°C
0.0697
NADH
pH 6.2, 25°C
0.0697
NADH
wild type enzyme, at pH 6.2 and 25°C
0.1054
NADH
pH 6.2, 25°C
0.1054
NADH
wild type enzyme, at pH 6.2 and 25°C
0.129
NADH
pH 6.2, 25°C, mutant Y225A
0.1299
NADH
mutant enzyme Y225A, at pH 6.2 and 25°C
0.132
NADH
pH 6.2, 25°C, mutant K245A
0.1323
NADH
mutant enzyme K245A, at pH 6.2 and 25°C
0.119
NADPH

recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate
0.119
NADPH
cosubstrate crotonyl-CoA, pH 6.2, 30°C
0.19
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0011
trans-2-dodecenoyl-CoA

mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication
0.003
trans-2-dodecenoyl-CoA
wild-type, pH not specified in the publication, temperature not specified in the publication
0.004
trans-2-dodecenoyl-CoA
mutant I287A, pH not specified in the publication, temperature not specified in the publication
0.0034
trans-2-hexenoyl-CoA

mutant I287A, pH not specified in the publication, temperature not specified in the publication
0.007
trans-2-hexenoyl-CoA
mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication
0.012
trans-2-hexenoyl-CoA
wild-type, pH not specified in the publication, temperature not specified in the publication
0.015
trans-2-hexenoyl-CoA
mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication
0.018
trans-2-hexenoyl-CoA
mutant Y370A, pH not specified in the publication, temperature not specified in the publication
0.019
trans-2-hexenoyl-CoA
mutant F295A, pH not specified in the publication, temperature not specified in the publication
0.038
trans-2-hexenoyl-CoA
mutant L291A, pH not specified in the publication, temperature not specified in the publication
0.091
trans-2-hexenoyl-CoA
pH 6.2, 30°C
0.091
trans-2-hexenoyl-CoA
recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor
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6 - 90
trans-2-dodecenoyl-CoA
15.3 - 112
trans-2-hexenoyl-CoA
6
2-dodecenoyl-CoA

pH and temperature not specified in the publication, mutant I287A
24
2-dodecenoyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A/F295A
90
2-dodecenoyl-CoA
pH and temperature not specified in the publication, wild-type
0.73
crotonyl-CoA

mutant Y240F, pH not specified in the publication, temperature not specified in the publication
0.73
crotonyl-CoA
pH and temperature not specified in the publication, mutant Y240F
7.1
crotonyl-CoA
mutant I287A, pH not specified in the publication, temperature not specified in the publication
7.1
crotonyl-CoA
pH and temperature not specified in the publication, mutant I287A
15.6
crotonyl-CoA
mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication
15.6
crotonyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A
18.9
crotonyl-CoA
mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication
18.9
crotonyl-CoA
pH and temperature not specified in the publication, mutant L276A/V277A/F295A
37
crotonyl-CoA
mutant L291A, pH not specified in the publication, temperature not specified in the publication
37
crotonyl-CoA
pH and temperature not specified in the publication, mutant L291A
73
crotonyl-CoA
mutant F295A, pH not specified in the publication, temperature not specified in the publication
73
crotonyl-CoA
pH and temperature not specified in the publication, mutant F295A
91
crotonyl-CoA
wild-type, pH not specified in the publication, temperature not specified in the publication