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(+)-cis-2',3'-dihydroxy-2',3'-dihydro-2-chlorobiphenyl + NAD+
2,3-dihydroxy-2'-chlorobiphenyl + NADH + H+
-
-
-
?
(+)-cis-2',3'-dihydroxy-2',3'-dihydro-3-chlorobiphenyl + NAD+
2,3-dihydroxy-3'-chlorobiphenyl + NADH + H+
-
-
-
?
(+)-cis-2',3'-dihydroxy-2',3'-dihydro-4-chlorobiphenyl + NAD+
2,3-dihydroxy-4'-chlorobiphenyl + NADH + H+
-
-
-
?
(1S)-1-indanol + NAD+
1-indanone + NADH + H+
with racemic 1-indanol, the enzyme enantioselectively transforms the (1S) enantiomer to 1-indanone
-
-
?
3,4-dichlorobenzene cis-dihydrodiol + NAD+
3,4-dichlorocatechol + NADH + H+
3,5-dichlorobenzene cis-dihydrodiol + NAD+
3,5-dichlorocatechol + NADH + H+
3,6-dichlorobenzene cis-dihydrodiol + NAD+
3,6-dichlorocatechol + NADH + H+
3,6-dimethylbenzene cis-dihydrodiol + NAD+
3,6-dimethylcatechol + NADH + H+
3-chloro-5-methylbenzene dihydrodiol + NAD+
3-chloro-5-methylcatechol + NADH + H+
-
-
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
3-chlorocatechol + NADH + H+
3-fluorobenzene cis-dihydrodiol + NAD+
3-fluorocatechol + NADH + H+
-
activity is 145% compared to the activity with benzene cis-dihydrodiol
-
-
?
3-methylbenzene cis-dihydrodiol + NAD+
3-methylcatechol + NADH + H+
-
activity is 185% compared to the activity with benzene cis-dihydrodiol
-
-
?
5-chloro-3-methylbenzene dihydrodiol + NAD+
5-chloro-3-methylcatechol + NADH + H+
-
-
-
-
?
benzene cis-dihydrodiol + NAD+
catechol + NADH + H+
-
-
-
-
?
cis-(1R,2S)-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene + NAD+
1,2-dihydroxy-3,4-dihydronaphthalene + NADH + H+
the enzyme oxidizes both enantiomers of cis-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene, but oxidation of the cis-(1S,2R) enantiomer is delayed until the cis-(1R,2S) enantiomer is completely depleted
-
-
?
cis-(1R,2S)-1,2-indandiol + NAD+
1,2-indenediol + NADH + H+
no activity with the cis-(1S,2R) enantiomer
-
-
?
cis-(1S,2R)-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene + NAD+
1,2-dihydroxy-3,4-dihydronaphthalene + NADH + H+
the enzyme oxidizes both enantiomers of cis-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene, but oxidation of the cis-(1S,2R) enantiomer is delayed until the cis-(1R,2S) enantiomer is completely depleted
-
-
?
cis-toluene dihydrodiol + NAD+
3-methylcatechol + NADH + H+
-
-
-
?
additional information
?
-
the enzyme also acts as an alcohol dehydrogenase
-
-
?
3,4-dichlorobenzene cis-dihydrodiol + NAD+
3,4-dichlorocatechol + NADH + H+
-
activity is 30% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,4-dichlorobenzene cis-dihydrodiol + NAD+
3,4-dichlorocatechol + NADH + H+
-
activity is 30% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,5-dichlorobenzene cis-dihydrodiol + NAD+
3,5-dichlorocatechol + NADH + H+
-
activity is 195% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,5-dichlorobenzene cis-dihydrodiol + NAD+
3,5-dichlorocatechol + NADH + H+
-
activity is 195% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,6-dichlorobenzene cis-dihydrodiol + NAD+
3,6-dichlorocatechol + NADH + H+
-
activity is 60% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,6-dichlorobenzene cis-dihydrodiol + NAD+
3,6-dichlorocatechol + NADH + H+
-
activity is 60% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,6-dimethylbenzene cis-dihydrodiol + NAD+
3,6-dimethylcatechol + NADH + H+
-
activity is 105% compared to the activity with benzene cis-dihydrodiol
-
-
?
3,6-dimethylbenzene cis-dihydrodiol + NAD+
3,6-dimethylcatechol + NADH + H+
-
activity is 105% compared to the activity with benzene cis-dihydrodiol
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
3-chlorocatechol + NADH + H+
-
activity is 170% compared to the activity with benzene cis-dihydrodiol
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
3-chlorocatechol + NADH + H+
-
activity is 170% compared to the activity with benzene cis-dihydrodiol
-
-
?
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Raschke, H.; Fleischmann, T.; Van Der Meer, J.R.; Kohler, H.P.
cis-chlorobenzene dihydrodiol dehydrogenase (TcbB) from Pseudomonas sp. strain P51, expressed in Escherichia coli DH5alpha(pTCB149), catalyzes enantioselective dehydrogenase reactions
Appl. Environ. Microbiol.
65
5242-5246
1999
Pseudomonas sp. P51 (Q52387)
brenda
Pollmann, K.; Beil, S.; Pieper, D.H.
Transformation of chlorinated benzenes and toluenes by Ralstonia sp. strain PS12 tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene dihydrodiol dehydrogenase) gene products
Appl. Environ. Microbiol.
67
4057-4063
2001
Cupriavidus sp. PS12 (O69264)
brenda
Spiess, E.; Goerisch, H.
Purification and characterization of chlorobenzene cis-dihydrodiol dehydrogenase from Xanthobacter flavus 14p1
Arch. Microbiol.
165
201-205
1996
Xanthobacter flavus, Xanthobacter flavus DSM 10330
brenda
Sommer, C.; Goerisch, H.
Enzymology of the degradation of (di)chlorobenzenes by Xanthobacter flavus 14p1
Arch. Microbiol.
167
384-391
1997
Xanthobacter flavus, Xanthobacter flavus DSM 10330
brenda
Pollmann, K.; Kaschabek, S.; Wray, V.; Reineke, W.; Pieper, D.H.
Metabolism of dichloromethylcatechols as central intermediates in the degradation of dichlorotoluenes by Ralstonia sp. strain PS12
J. Bacteriol.
184
5261-5274
2002
Cupriavidus sp. PS12 (O69264)
brenda
Pollmann, K.; Wray, V.; Pieper, D.H.
Chloromethylmuconolactones as critical metabolites in the degradation of chloromethylcatechols recalcitrance of 2-chlorotoluene
J. Bacteriol.
187
2332-2340
2005
Cupriavidus sp. PS12
brenda
Werlen, C.; Kohler, H.P.; van der Meer, J.R.
The broad substrate chlorobenzene dioxygenase and cis-chlorobenzene dihydrodiol dehydrogenase of Pseudomonas sp. strain P51 are linked evolutionarily to the enzymes for benzene and toluene degradation
J. Biol. Chem.
271
4009-4016
1996
Pseudomonas sp. P51 (Q52387)
brenda
Kunze, M.; Zerlin, K.F.; Retzlaff, A.; Pohl, J.O.; Schmidt, E.; Janssen, D.B.; Vilchez-Vargas, R.; Pieper, D.H.; Reineke, W.
Degradation of chloroaromatics by Pseudomonas putida GJ31 assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome
Microbiology
155
4069-4083
2009
Pseudomonas putida GJ31 (Q49KD4), Pseudomonas putida GJ31
brenda
1.3.1.119 chlorobenzene dihydrodiol dehydrogenase
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