Information on EC 1.3.1.116 - 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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The expected taxonomic range for this enzyme is: Clostridium scindens

EC NUMBER
COMMENTARY hide
1.3.1.116
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RECOMMENDED NAME
GeneOntology No.
7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Secondary bile acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
7beta-hydroxy-3-oxochol-24-oyl-CoA DELTA4-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
FAD + NADH + H+
FADH2 + NAD+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no cofactor: NADP+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
-
1 mol of copper per mol of subunit
Iron
-
2 mol of iron per mol of subunit
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Acriflavine
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0.2 mM, 13% residual activity
Cu2+
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1 mM, 1% residual activity
EDTA
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5 mM, 52% residual activity
N-bromosuccinimide
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0.2 mM, 8% residual activity
N-ethylmaleimide
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1 mM, 4% residual activity
o-phenanthroline
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5 mM, 58% residual activity
p-Chloromercuriphenyl sulfonate
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0.2 mM, 5% residual activity
Zn2+
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1 mM, 27% residual activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16.4
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pH 6.8, 25°C
48.36
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pH 6.8, 20°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
210000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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