Information on EC 1.3.1.114 - 3-dehydro-bile acid DELTA4,6-reductase

for references in articles please use BRENDA:EC1.3.1.114
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Clostridium scindens

EC NUMBER
COMMENTARY hide
1.3.1.114
-
RECOMMENDED NAME
GeneOntology No.
3-dehydro-bile acid DELTA4,6-reductase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ = 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
show the reaction diagram
(4)
-
-
-
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ = 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
(3)
-
-
-
3-oxochol-4-en-24-oyl-CoA + NAD+ = 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
show the reaction diagram
(2)
-
-
-
3-oxocholan-24-oyl-CoA + NAD+ = 3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
(1)
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acids degradation
-
-
Secondary bile acid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3-oxocholan-24-oyl-CoA DELTA4,6-oxidoreductase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+
12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
show the reaction diagram
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
3-oxochol-4-en-24-oyl-CoA + NAD+
3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
show the reaction diagram
3-oxocholan-24-oyl-CoA + NAD+
3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+
12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
show the reaction diagram
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
3-oxochol-4-en-24-oyl-CoA + NAD+
3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
show the reaction diagram
3-oxocholan-24-oyl-CoA + NAD+
3-oxochol-4-en-24-oyl-CoA + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified protein is stable only for less than 12 h, then precipitates
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol does not improve stability
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli