The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that usually further reduces the product to (+)-secoisolariciresinol [EC 184.108.40.206, (-)-lariciresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) , Linum perenne (perennial flax) and Arabidopsis thaliana (thale cress) .
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The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that usually further reduces the product to (+)-secoisolariciresinol [EC 220.127.116.11, (-)-lariciresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) , Linum perenne (perennial flax)  and Arabidopsis thaliana (thale cress) .
the parenchymatous cell layer of the outer integument of flaxseed coats, the enzyme is strongly expressed in the seed coats of developing flaxseed, quantitative RT-PCR expression analysis or recombinant PLR1 promoter construct in transgenic flaxseed
RNAi-generated plants exhibit LuPLR1 gene silencing and fail to accumulate secoisolariciresinol diglucoside (SDG). The accumulation of pinoresinol, the substrate of the PLR1 enzyme, in its diglucosylated form (PDG) is increased in transgenic seeds but does not compensate the overall loss of SDG. The monolignol flux is also deviated through the synthesis of 8-5'-linked neolignans dehydrodiconiferyl alcohol glucoside (DCG) and dihydro-dehydrodiconiferyl alcohol glucoside (DDCG)
the loss-of-function mutant of PrR1 shows, in addition to elevated levels of pinoresinol, significantly decreased lignin content and a slightly altered lignin structure with lower abundance of cinnamyl alcohol end groups. Stimulated Raman scattering (SRS) microscopy analysis indicates that the lignin content of the prr1-1 loss-of-function mutant is similar to that of wild-type plants in xylem cells, which exhibit a normal phenotype, but is reduced in the fiber cells. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin. The PrR1 isozyme mutation alters the expression level of diverse genes, overview
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
the enzyme is involved in the biosynthetic pathway of secoisolariciresinol diglucoside in flax Linum usitatissimum, overview. The correlation coefficient between LuPLR1 gene expression and lignan yatein accumulation is low
recombinant expression of gene pinZ in Arabidopsis thaliana causes dynamic metabolic changes in stems, but not in roots and leaves. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers
the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, responsible for the synthesis of the enantiomer (+)-secoisolariciresinol, spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview
pinoresinol reductase (PrR) catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. Association of the lignan biosynthetic enzyme encoded by PrR1 with secondary cell wall biosynthesis in fiber cells. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46. PrR1 plays a role in lignan biosynthesis in aerial tissue
the prr1-1 knock-out mutant SALK_058467 contains a T-DNA insertion within an exonic region of the PrR1 gene. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin
RNAi-mediated pinoresinol lariciresinol reductase gene silencing, via Agrobacterium tumefaciens strain GV301 transformation, in Linum usitatissimum seed coat with consequences on lignans and neolignans accumulation, LuPLR1 transcript level is strongly reduced in the seeds ofPLR-RNAi plants, phenotype, overview
gene pinZ, recombinant expression in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter, pinZ expression causes dynamic metabolic changes in stems, but not in roots and leaves. Accumulation of the glucoside of secoisolariciresinol appears to be elevated in the transgenic plant. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers, recombinant enzyme tissue expression pattern in plant seedlings
gene PrR1, PrR1 is co-expressed with many characterized genes involved in secondary cell wall biosynthesis, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46
the plasmid is transferred into the disarmed Agrobacter tumefaciens strain GV3101 by triparental mating with Escherichia coli strain HB101 and then introduced into Linum usitatissimum (cv. Barbara) transgenic plants
the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis
the expression of the PLR1 gene in the seed coat is up-regulated about 3fold by 0.1 mM exogenous abscisic acid. There is a high mid-maturation expression of PLR1 gene between developmental stage 2 and stage