Information on EC 1.23.1.2 - (+)-lariciresinol reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.23.1.2
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RECOMMENDED NAME
GeneOntology No.
(+)-lariciresinol reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(-)-secoisolariciresinol + NADP+ = (+)-lariciresinol + NADPH + H+
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
justicidin B biosynthesis
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matairesinol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(–)-secoisolariciresinol:NADP+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that also reduces (+)-pinoresinol [EC 1.23.1.1, (+)-pinoresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) [3], Linum perenne (perennial flax) [5] and Linum corymbulosum [6].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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G2IMF2
UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
additional information
analysis of transcriptional regulation of the LuPLR2 gene from flax, overview. Spatiotemporal LuPLR2 gene expression pattern in relation to yatein biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-lariciresinol + NADPH + H+
(-)-secoisolariciresinol + NADP+
show the reaction diagram
(+)-lariciresinol + NADPH + H+
secoisolariciresinol + NADP+
show the reaction diagram
(+)-pinoresinol + NADPH + H+
(+)-lariciresinol + NADP+
show the reaction diagram
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-
-
?
medioresinol + NADPH + H+
? + NADP+
show the reaction diagram
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-
-
-
?
syringaresinol + NADPH + H+
? + NADP+
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-lariciresinol + NADPH + H+
(-)-secoisolariciresinol + NADP+
show the reaction diagram
(+)-pinoresinol + NADPH + H+
(+)-lariciresinol + NADP+
show the reaction diagram
E6Y2X0
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-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000083
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crude extract, at pH 7.4 and 30°C
0.255
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after 3060fold purification, at pH 7.4 and 30°C
131.4
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using (+)-secoisolariciresinol and [4R]-NADPH as substrates, at pH 8.0 and 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
G2IMF2;
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
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isoelectric focusing
7.1
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calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
identification of subcellular location of LuPLR2-derived lignan biosynthesis transgenic tobacco mesophyll cells that express LuPLR2-eGFP fusion proteins, LuPLR2 gene expression analysis
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36400
x * 36400, calculated from amino acid sequence
39400
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x * 39400, calculated from amino acid sequence
59000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 36400, calculated from amino acid sequence
monomer or dimer
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x * 35000-36000, SDS-PAGE; x * 39400, calculated from amino acid sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, Affinity Blue Gel filtration, phenyl Sepharose column chromatography, hydroxyapatite column chromatography, 2’,5’ ADP-Sepharose column chromatography, Affinity-Yellow gel filtration, and Superose 12 gel filtration
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ammonium sulfate precipitation and Affi-Gel Blue Gel column chromatography
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Ni-NTA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli Nova Blue cells
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expressed in Escherichia coli Nova-Blue cells
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gene pinZ, recombinant expression in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter, pinZ expression causes dynamic metabolic changes in stems, but not in roots and leaves. Accumulation of the glucoside of secoisolariciresinol appears to be elevated in the transgenic plant. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers, recombinant enzyme tissue expression pattern in plant seedlings
G2IMF2;
gene PLR2, recombinant expression of N-terminal and C-terminal fusions of LuPLR2 with EGFP or GUS in transgenic tobacco mesophyll cells, and LuPLR2 overexpression in transgenic flax plants, via transformation by Agrobacterium tumefaciens strain GV3101, quantitative RT-PCR expression analysis of LuPLR1 and LuPLR2 expressions in leaves and seeds, subcloning in Escherichia coli strain HB101. cis-Elements, responsible for gene expression in response to stress, are located in separate portions of the LuPLR2 promoter, presence of two MYB-binding sites. Quantitative RT-PCR expression analysis of LuPLR2 in transgenic seedlings, analysis of the transcriptional regulation of the LuPLR2 gene
gene PrP2, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR2 expression clusters with a different set of genes, not involved in secondary cell wall biosynthesis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
analysis of transcriptional regulation of the LuPLR2 gene from flax by a PCR walking strategy, overview
methyljasmonate triggers the expression of LuPLR2 leading to an over-accumulation of yatein, while salicylic acid fails to act strongly on the expression of this gene and yatein accumulation is not stimulated. LuPLR2 gene expression shows an increase in wounded plants, LuPLR2 gene expression is induced in wounded leaves compared to the control, cis-elements, responsible for gene expression in response to stress, are located in separate portions of the LuPLR2 promoter. LuPLR2 expression and yatein production are increased by methyl jasmonate and wounding
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information