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Information on EC 1.2.99.8 - glyceraldehyde dehydrogenase (FAD-containing)
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1.2.99.8 glyceraldehyde dehydrogenase (FAD-containing)IUBMB Comments
The enzyme from the archaeon Sulfolobus acidocaldarius catalyses the oxidation of D-glyceraldehyde in the nonphosphorylative Entner-Doudoroff pathway. With 2,6-dichlorophenolindophenol as artificial electron acceptor, the enzyme shows a broad substrate range, but is most active with D-glyceraldehyde. It is not known which acceptor is utilized in vivo. The iron-sulfur protein contains FAD and molybdopterin guanine dinucleotide.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
Gaor1, Gaor2, Gaor3, glyceraldehyde oxidoreductase, ST0561, ST0562, ST1781, ST1782, ST1783, ST2339, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Gaor1
Gaor2
Gaor3
glyceraldehyde oxidoreductase
ST0561
ST0562
ST1781
ST1782
ST1783
ST2339
ST2484
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-glyceraldehyde + H2O + acceptor = D-glycerate + reduced acceptor
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde:acceptor oxidoreductase (FAD-containing)
The enzyme from the archaeon Sulfolobus acidocaldarius catalyses the oxidation of D-glyceraldehyde in the nonphosphorylative Entner-Doudoroff pathway. With 2,6-dichlorophenolindophenol as artificial electron acceptor, the enzyme shows a broad substrate range, but is most active with D-glyceraldehyde. It is not known which acceptor is utilized in vivo. The iron-sulfur protein contains FAD and molybdopterin guanine dinucleotide.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetaldehyde + 2,6-dichlorophenolindophenol + H2O
acetate + reduced 2,6-dichlorophenolindophenol
D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol
D-glycerate + reduced 2,6-dichlorophenolindophenol
D-glyceraldehyde-3-phosphate + H2O + methyl viologen
3-phospho-D-glycerate + reduced methyl viologen
DL-glyceraldehyde + 2,6-dichlorophenolindophenol + H2O
glycerate + reduced 2,6-dichlorophenolindophenol
formaldehyde + 2,6-dichlorophenolindophenol + H2O
formate + reduced 2,6-dichlorophenolindophenol
glyceraldehyde-3-phosphate + 2,6-dichlorophenolindophenol + H2O
3-phospho-D-glycerate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibits activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O
indoleacetate + reduced 2,6-dichlorophenolindophenol
indolepyruvate + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii
-
-
-
?
isobutyraldehyde + 2,6-dichlorophenolindophenol + H2O
isobutyrate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibits activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
propionaldehyde + 2,6-dichlorophenolindophenol + H2O
propionate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibited activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
acetaldehyde + 2,6-dichlorophenolindophenol + H2O
acetate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibited activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
acetaldehyde + 2,6-dichlorophenolindophenol + H2O
acetate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius DSM 639
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibited activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
acetaldehyde + 2,6-dichlorophenolindophenol + H2O
acetate + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii
-
-
-
?
acetaldehyde + 2,6-dichlorophenolindophenol + H2O
acetate + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii DSM 16993
-
-
-
?
D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol
D-glycerate + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii
-
-
-
?
D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol
D-glycerate + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii DSM 16993
-
-
-
?
D-glyceraldehyde + H2O + acceptor
D-glycerate + reduced acceptor
Sulfolobus acidocaldarius
function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway
-
-
?
D-glyceraldehyde + H2O + acceptor
D-glycerate + reduced acceptor
Sulfolobus acidocaldarius DSM 639
function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway
-
-
?
D-glyceraldehyde + H2O + methyl viologen
D-glycerate + reduced methyl viologen
Sulfurisphaera tokodaii
-
-
-
?
D-glyceraldehyde + H2O + methyl viologen
D-glycerate + reduced methyl viologen
Sulfurisphaera tokodaii DSM 16993
-
-
-
?
D-glyceraldehyde-3-phosphate + H2O + methyl viologen
3-phospho-D-glycerate + reduced methyl viologen
Sulfurisphaera tokodaii
-
-
-
?
D-glyceraldehyde-3-phosphate + H2O + methyl viologen
3-phospho-D-glycerate + reduced methyl viologen
Sulfurisphaera tokodaii DSM 16993
-
-
-
?
DL-glyceraldehyde + 2,6-dichlorophenolindophenol + H2O
glycerate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibits activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
DL-glyceraldehyde + 2,6-dichlorophenolindophenol + H2O
glycerate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius DSM 639
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibits activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
formaldehyde + 2,6-dichlorophenolindophenol + H2O
formate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibits activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
formaldehyde + 2,6-dichlorophenolindophenol + H2O
formate + reduced 2,6-dichlorophenolindophenol
Sulfolobus acidocaldarius DSM 639
none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibits activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate
-
-
?
indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O
indoleacetate + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii
-
-
-
?
indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O
indoleacetate + reduced 2,6-dichlorophenolindophenol
Sulfurisphaera tokodaii DSM 16993
-
-
-
?
additional information
?
-
Sulfolobus acidocaldarius
no activity with D-glucose. None of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol
-
-
?
additional information
?
-
Sulfolobus acidocaldarius
-
no activity with D-glucose. None of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol
-
-
?
additional information
?
-
Sulfolobus acidocaldarius DSM 639
no activity with D-glucose. None of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde + H2O + acceptor
D-glycerate + reduced acceptor
Sulfolobus acidocaldarius
function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
molybdopterin cytosine dinucleotide
Sulfurisphaera tokodaii
-
molybdopterin guanine dinucleotide
Sulfolobus acidocaldarius
contains 1 molybdopterin guanine dinucleotide per enzyme molecule
[2Fe-2S]-center
Sulfurisphaera tokodaii
small subunit contains two [2Fe-2S] clusters
FAD
Sulfurisphaera tokodaii
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
copper
Iron
Molybdenum
copper
Sulfurisphaera tokodaii
0.3 nmol/mg protein
copper
Sulfurisphaera tokodaii
0.56 nmol/mg protein
copper
Sulfurisphaera tokodaii
0.76 nmol/mg protein
Iron
Sulfolobus acidocaldarius
contains either one [4Fe-4S]-cluster or two [2Fe-2S]-clusters per molecule
Iron
Sulfurisphaera tokodaii
10.5 nmol/mg protein
Iron
Sulfurisphaera tokodaii
18.6 nmol/mg protein
Iron
Sulfurisphaera tokodaii
26.2 nmol/mg protein
Molybdenum
Sulfurisphaera tokodaii
2.9 nmol/mg protein
Molybdenum
Sulfurisphaera tokodaii
4.46 nmol/mg protein
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.86
Indolepyruvate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.24
acetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
1.33
acetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.011
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C
0.018
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.03
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C
0.091
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.138
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C
0.63
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.0035
D-Glyceraldehyde-3-phosphate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.012
D-Glyceraldehyde-3-phosphate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.085
D-Glyceraldehyde-3-phosphate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.037
Indoleacetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.092
Indoleacetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
11
Indolepyruvate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
3.23
acetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
3.38
acetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.72
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
3.58
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C
3.78
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C
5.23
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
11.2
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
50.5
D-glyceraldehyde
Sulfurisphaera tokodaii
cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C
0.43
D-Glyceraldehyde-3-phosphate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.65
D-Glyceraldehyde-3-phosphate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
0.98
D-Glyceraldehyde-3-phosphate
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
2.77
Indoleacetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
3.46
Indoleacetaldehyde
Sulfurisphaera tokodaii
cosubstrate methyl viologen, pH 7.0, 70°C
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Sulfurisphaera tokodaii
Sulfurisphaera tokodaii DSM 16993
Sulfurisphaera tokodaii
F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7
UniProt
brenda
Sulfurisphaera tokodaii
Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
Q96XN5; Q974U9; Q974V0
UniProt
brenda
Sulfurisphaera tokodaii
Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
Q96Y29; Q974U9; Q974V0
UniProt
brenda
Sulfurisphaera tokodaii DSM 16993
F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7
UniProt
brenda
Sulfurisphaera tokodaii DSM 16993
Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
Q96XN5; Q974U9; Q974V0
UniProt
brenda
Sulfurisphaera tokodaii DSM 16993
Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
Q96Y29; Q974U9; Q974V0
UniProt
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Sulfolobus acidocaldarius
present in the cytosol to at least 0.4% of the soluble protein
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
Sulfolobus acidocaldarius
function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway
physiological function
Sulfolobus acidocaldarius DSM 639
-
function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
CUTA_SULAC
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
748
0
82572
Swiss-Prot
CUTC_SULAC
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
163
0
17856
Swiss-Prot
CUTB_SULAC
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
281
0
30863
Swiss-Prot
A0A564Q9Z1_9EURY
Candidatus Methanolliviera sp. GoM_oil
274
0
30475
TrEMBL
A0A1Q9ND78_9ARCH
Candidatus Heimdallarchaeota archaeon LC_3
478
0
53998
TrEMBL
A0A1Q9NDK4_HEILC
Heimdallarchaeota archaeon (strain LC_2)
483
0
53852
TrEMBL
A0A484HE52_9DELT
uncultured Desulfobacteraceae bacterium
161
0
17258
TrEMBL
A0A0J8DD11_CLOCY
Clostridium cylindrosporum DSM 605
159
0
17350
TrEMBL
A0A0H3IZH6_CLOPA
Clostridium pasteurianum DSM 525 = ATCC 6013
287
0
32283
TrEMBL
A0A1Q9ND68_9ARCH
Candidatus Heimdallarchaeota archaeon LC_3
278
0
31364
TrEMBL
A0A1Q9NT51_HEILC
Heimdallarchaeota archaeon (strain LC_2)
274
0
31492
TrEMBL
A0A0J8DCW0_CLOCY
Clostridium cylindrosporum DSM 605
158
0
17228
TrEMBL
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
19500
Sulfolobus acidocaldarius
x * 80500 + x * 32000 + x * 19500, the exact subunit composition (alphabeta(x)gamma(y)) could not be determined, SDS-PAGE
20000
Sulfurisphaera tokodaii
-
200000
Sulfurisphaera tokodaii
gel filtration
21000
Sulfurisphaera tokodaii
-
29000
Sulfurisphaera tokodaii
-
30000
Sulfurisphaera tokodaii
-
32000
Sulfolobus acidocaldarius
x * 80500 + x * 32000 + x * 19500, the exact subunit composition (alphabeta(x)gamma(y)) could not be determined, SDS-PAGE
372000
Sulfurisphaera tokodaii
gel filtration
80500
Sulfolobus acidocaldarius
x * 80500 + x * 32000 + x * 19500, the exact subunit composition (alphabeta(x)gamma(y)) could not be determined, SDS-PAGE
81000
Sulfurisphaera tokodaii
-
82000
Sulfurisphaera tokodaii
-
83000
Sulfurisphaera tokodaii
-
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dodecamer
heterohexamer
heterotrimer
?
Sulfolobus acidocaldarius
x * 80500 + x * 32000 + x * 19500, the exact subunit composition (alphabeta(x)gamma(y)) could not be determined, SDS-PAGE
?
Sulfolobus acidocaldarius DSM 639
-
x * 80500 + x * 32000 + x * 19500, the exact subunit composition (alphabeta(x)gamma(y)) could not be determined, SDS-PAGE
dodecamer
Sulfurisphaera tokodaii
4 * 83000, gene product ST1781, plus 4 * 29000, gene product ST1783, plus 4 * 20000, gene product ST1782. Isoform Gaor1 is a tetramer of the gene products ST1781/ST1783/ST1782 hetero-trimer
dodecamer
Sulfurisphaera tokodaii DSM 16993
-
4 * 83000, gene product ST1781, plus 4 * 29000, gene product ST1783, plus 4 * 20000, gene product ST1782. Isoform Gaor1 is a tetramer of the gene products ST1781/ST1783/ST1782 hetero-trimer
heterohexamer
Sulfurisphaera tokodaii
2 * 81000, gene product ST2339, plus 2 * 30000, gene product ST0562, plus 2 * 21000, gene product ST0561. Isoform Gaor1 is a dimer of the gene products ST1781/ST1783/ST1782 heterotrimer
heterohexamer
Sulfurisphaera tokodaii DSM 16993
-
2 * 81000, gene product ST2339, plus 2 * 30000, gene product ST0562, plus 2 * 21000, gene product ST0561. Isoform Gaor1 is a dimer of the gene products ST1781/ST1783/ST1782 heterotrimer
heterotrimer
Sulfurisphaera tokodaii
1 * 82000, gene product ST2484, plus 1 * 30000, gene product ST0562, plus 1 * 21000, gene product ST0561
heterotrimer
Sulfurisphaera tokodaii DSM 16993
-
1 * 82000, gene product ST2484, plus 1 * 30000, gene product ST0562, plus 1 * 21000, gene product ST0561
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
phosphoprotein
Sulfolobus acidocaldarius
phosphate content of 3.7 phosphates per molecule of protei
phosphoprotein
Sulfolobus acidocaldarius DSM 639
-
phosphate content of 3.7 phosphates per molecule of protei
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 2.2 A resolution. The asymmetric unit contains one hetero-trimer, one Mo-PCD (large subunit), one FAD (medium subunit), two [2Fe-2S] clusters (designated as I and II, small subunit), and solvent molecules
Sulfurisphaera tokodaii
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
92
Sulfolobus acidocaldarius
apparent melting temperature, in association with other proteins the enzyme must be even more stable since it survived the prolonged heat treatment during the purification procedure
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kardinahl, S.; Schmidt, C.L.; Hansen, T.; Anemueller, S.; Petersen, A.; Schaefer, G.
The strict molybdate-dependence of glucose-degradation by the thermoacidophile Sulfolobus acidocaldarius reveals the first crenarchaeotic molybdenum containing enzyme - an aldehyde oxidoreductase
Eur. J. Biochem.
260
540-548
1999
Sulfolobus acidocaldarius (Q4J6M3 AND Q4J6M6 AND Q4J6M5
), Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639 (Q4J6M3 AND Q4J6M6 AND Q4J6M5
)
brenda
Wakagi, T.; Nishimasu, H.; Miyake, M.; Fushinobu, S.
Archaeal Mo-containing glyceraldehyde oxidoreductase isozymes exhibit diverse substrate specificities through unique subunit assemblies
PLoS ONE
11
e0147333
2016
Sulfurisphaera tokodaii (F9VNL4 and F9VNL6 and F9VNL5), Sulfurisphaera tokodaii (Q96XN5 and Q974U9 and Q974V0), Sulfurisphaera tokodaii (Q96Y29 and Q974U9 and Q974V0), Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993 (F9VNL4 and F9VNL6 and F9VNL5), Sulfurisphaera tokodaii DSM 16993 (Q96XN5 and Q974U9 and Q974V0), Sulfurisphaera tokodaii DSM 16993 (Q96Y29 and Q974U9 and Q974V0)
brenda
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