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EC Tree
IUBMB Comments Catalyses the NADPH-dependent reduction of succinyl-CoA to succinate semialdehyde. The enzyme has been described in Clostridium kluyveri, where it participates in succinate fermentation , and in Metallosphaera sedula, where it participates in the 3-hydroxypropanonate/4-hydroxybutanoate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [2,3].
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
Tneu_0421,
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Tneu_0421
locus name
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succinate semialdehyde + CoA + NADP+ = succinyl-CoA + NADPH + H+
succinate semialdehyde + CoA + NADP+ = succinyl-CoA + NADPH + H+
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succinate semialdehyde + CoA + NADP+ = succinyl-CoA + NADPH + H+
ping-pong mechanism
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succinate semialdehyde:NADP+ oxidoreductase (CoA-acylating)
Catalyses the NADPH-dependent reduction of succinyl-CoA to succinate semialdehyde. The enzyme has been described in Clostridium kluyveri, where it participates in succinate fermentation [1], and in Metallosphaera sedula, where it participates in the 3-hydroxypropanonate/4-hydroxybutanoate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [2,3].
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caproaldehyde + coenzyme A + NADP+
n-hexanoyl-CoA + NADPH + H+
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at 8.6% of the rate with succinate-semialdehyde
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r
glutaryl-CoA + NADPH + H+
? + coenzyme A + NADP+
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at 6.7% of the rate with succinyl-coA
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r
glycolaldehyde + coenzyme A + NADP+
? + NADPH + H+
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at 9.1% of the rate with succinate-semialdehyde
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r
n-butyraldehyde + coenzyme A + NADP+
n-butyryl-CoA + NADPH + H+
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at 1% of the rate with succinate-semialdehyde
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r
n-hexanoyl-CoA + NADPH + H+
caproaldehyde + coenzyme A + NADP+
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at 8.1% of the rate with succinyl-coA
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r
palmitoyl-CoA + NADPH + H+
? + coenzyme A + NADP+
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at 15.1% of the rate with succinyl-coA
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r
succinate-semialdehyde + coenzyme A + NADP+
succinyl-CoA + NADPH + H+
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r
succinyl-CoA + NADPH + H+
succinate-semialdehyde + coenzyme A + NADP+
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r
valeraldehyde + coenzyme A + NADP+
n-valeryl-CoA + NADPH + H+
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at 6.0% of the rate with succinate-semialdehyde
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r
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2.9
coenzyme A
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pH 8.5, 30°C
4.3
NADPH
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pH 7.0, 30°C
2.7
Succinate-semialdehyde
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pH 8.5, 30°C
3.2
succinyl-CoA
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pH 7.0, 30°C
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7
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reduction of succinyl-CoA
8.5
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oxidation of succinate-semialdehyde
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UniProt
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UniProt
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metabolism
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the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
physiological function
the enzyme is involved in autotrophic CO2 fixation
physiological function
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the enzyme is involved in autotrophic CO2 fixation
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115000
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sucrose gradient centrifugation
55000
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2 * 55000, SDS-PAGE
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dimer
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2 * 55000, SDS-PAGE
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enzyme is air-sensitive. Exposure to air at room temperature or 4°C results in a complete inactivation within 6 h or 24 h, respectively
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expression in Escherichia coli
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approximately 10fold downregulated in presence of acetate, 4-hydroxybutyrate, succinate or pyruvate
extracts of autotrophically grown cells exhibit an NADPH-dependent succinyl-CoA reductase activity of 200 nM/min*mg of soluble protein, whereas extracts of heterotrophically grown cells exhibit an activity of 10 nM/min*mg of protein
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approximately 10fold downregulated in presence of acetate, 4-hydroxybutyrate, succinate or pyruvate
approximately 10fold downregulated in presence of acetate, 4-hydroxybutyrate, succinate or pyruvate
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Sohling, B.; Gottschalk, G.
Purification and characterization of a coenzyme-A-dependent succinate-semialdehyde dehydrogenase from Clostridium kluyveri
Eur. J. Biochem.
212
121-127
1993
Clostridium kluyveri
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Ramos-Vera, W.H.; Labonte, V.; Weiss, M.; Pauly, J.; Fuchs, G.
Regulation of autotrophic CO2 fixation in the archaeon Thermoproteus neutrophilus
J. Bacteriol.
192
5329-5340
2010
Pyrobaculum neutrophilum (B1YBY5), Pyrobaculum neutrophilum DSM 2338 (B1YBY5)
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1.2.1.76 succinate-semialdehyde dehydrogenase (acylating)
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