We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.2.1.60 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase for references in articles please use BRENDA:EC1.2.1.60
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Involved in the tyrosine degradation pathway in Arthrobacter sp.
The enzyme appears in viruses and cellular organisms
Synonyms
5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase
-
-
-
-
5-carboxymethyl-2-hydroxymuconate semialdehyde:NAD+ oxidoreductase
-
-
-
-
5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase
-
-
-
-
carboxymethylhydroxymuconic semialdehyde dehydrogenase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ = 5-carboxymethyl-2-hydroxymuconate + NADH + 2 H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-carboxymethyl-2-hydroxymuconic-semialdehyde:NAD+ oxidoreductase
Involved in the tyrosine degradation pathway in Arthrobacter sp.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
-
11% of the activity compared to the reduction with NAD+
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
-
1,5% of the activity compared to reduction with NAD+
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
-
1,5% of the activity compared to reduction with NAD+
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
-
1,5% of the activity compared to reduction with NAD+
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
-
18% of the activity compared to reduction with NAD+
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
-
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
-
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+
-
can not be replaced by NADP+
NAD+
-
strict dependency for NAD+ reduction
NAD+
-
strict dependency for NAD+ reduction
NADP+
-
82% lower activity compared to NAD+
NADP+
-
89% lower activity compared to NAD+
NADP+
-
98.5% lower activity compared to NAD+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP
-
competitive inhibition, Ki: 0.088 mM
m-hydroxybenzaldehyde
-
-
p-Hydroxybenzaldehyde
-
-
NADH
-
competitive inhibition, Ki: 0.025 mM
NADH
-
competitive inhibition with respect to NAD+, Ki: 0.027 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.005 - 0.009
5-carboxymethyl-2-hydroxymuconate semialdehyde
0.005
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
-
0.006
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
-
0.009
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
-
0.009
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
-
0.009
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
-
0.012
NAD+
-
-
1.2
NADP+
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
40
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
54
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5
-
-
7.8
-
less active in Tris-HCl buffer than in phosphate or glycine/NaOH buffers
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
-
brenda
-
-
brenda
-
;
-
brenda
-
;
-
brenda
-
-
brenda
-
-
-
brenda
-
-
brenda
-
strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate
brenda
-
strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
52500
-
3 * 52500, SDS-PAGE
58000
-
4 * 58000, SDS-PAGE
52000
-
2 * 52000, SDS-PAGE
52000
-
2 * 52000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
-
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
-
tetramer
-
4 * 58000, SDS-PAGE
tetramer
-
3 * 52500, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
42
-
loss of activity after 10 min
47
-
complete loss of activity after 20 min
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
to homogeneity, chromatography techniques
to homogeneity, chromatography techniques
-
to homogeneity, chromatography techniques
-
to homogeneity, chromatography techniques
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Blakley, E.R.
The catabolism of L-tyrosine by an Arthrobacter sp.
Can. J. Microbiol.
23
1128-1139
1977
Arthrobacter globiformis, Arthrobacter globiformis PRL W15
brenda
Fawcett, T.; Garrido-Pertierra, A.; Cooper, R.A.
5-Carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenases of Escherichia coli C and Klebsiella pneumoniae M5a1 show very high N-terminal sequence homology
FEMS Microbiol. Lett.
57
307-312
1989
Escherichia coli, Escherichia coli C, Klebsiella pneumoniae, Klebsiella pneumoniae M5a1
brenda
Alonso, J.M.; Garrido-Pertierra, A.
Carboxymethylhydroxymuconic semialdehyde dehydrogenase in the 4-hydroxyphenylacetate catabolic pathway of Pseudomonas putida
Biochem. Cell Biol.
64
1288-1293
1986
Pseudomonas putida
-
brenda
Alonso, J.M.; Garrido-Pertierra, A.
Kinetic properties of 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase from Escherichia coli
Biochimie
68
731-737
1986
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Alonso, J.M.; Garrido-Pertierra, A.
Carboxymethylhydroxymuconic semialdehyde dehydrogenase in the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli
Biochim. Biophys. Acta
719
165-167
1982
Escherichia coli
brenda
Cooper, R.A.; Skinner, M.A.
Catabolism of 3- and 4-hydroxyphenylacetate by the 3,4-dihydroxyphenylacetate pathway in Escherichia coli
J. Bacteriol.
143
302-306
1980
Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia coli C
brenda
Barbour, M.G.; Bayly, R.C.
Control of meta-cleavage degradation of 4-hydroxyphenylacetate in Pseudomonas putida
J. Bacteriol.
147
844-850
1981
Pseudomonas putida, Pseudomonas putida NCIB 9865
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.2.1.60 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase
more
top
Information
