Information on EC 1.2.1.60 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase

for references in articles please use BRENDA:EC1.2.1.60
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.2.1.60
-
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RECOMMENDED NAME
GeneOntology No.
5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ = 5-carboxymethyl-2-hydroxymuconate + NADH + 2 H+
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-hydroxyphenylacetate degradation
Tyrosine metabolism
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-
Microbial metabolism in diverse environments
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-
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SYSTEMATIC NAME
IUBMB Comments
5-carboxymethyl-2-hydroxymuconic-semialdehyde:NAD+ oxidoreductase
Involved in the tyrosine degradation pathway in Arthrobacter sp.
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CAS REGISTRY NUMBER
COMMENTARY hide
63241-20-3
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Escherichia coli B / ATCC 11303
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
show the reaction diagram
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
competitive inhibition, Ki: 0.088 mM
m-hydroxybenzaldehyde
-
-
p-Hydroxybenzaldehyde
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.009
5-carboxymethyl-2-hydroxymuconate semialdehyde
0.012 - 0.052
NAD+
1.2 - 3.6
NADP+
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40
5-carboxymethyl-2-hydroxymuconate semialdehyde
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52500
-
3 * 52500, SDS-PAGE
58000
-
4 * 58000, SDS-PAGE
102000
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gel filtration
125000
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gel filtration
200000
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gel filtration
210000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable for 30 min
37
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stable for 30 min
42
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loss of activity after 10 min
47
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complete loss of activity after 20 min
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
frozen, 12 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
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Show AA Sequence (335 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 1.2.1.60)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)