Any feedback?
No. of entries
Information on EC 1.2.1.49 - 2-oxoaldehyde dehydrogenase (NADP+)
for references in articles please use BRENDA:EC1.2.1.49
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 1 Oxidoreductases
1.2 Acting on the aldehyde or oxo group of donors
1.2.1 With NAD+ or NADP+ as acceptor
1.2.1.49 2-oxoaldehyde dehydrogenase (NADP+)
IUBMB Comments
Not identical with EC 1.2.1.23 2-oxoaldehyde dehydrogenase (NAD+).
The enzyme appears in viruses and cellular organisms
showSynonyms
2-ketoaldehyde dehydrogenase, alpha-ketoaldehyde dehydrogenase, methylglyoxal dehydrogenase, NADP-dependent alpha-ketoaldehyde dehydrogenase, NADP-linked alpha-ketoaldehyde dehydrogenase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-ketoaldehyde dehydrogenase
-
-
-
-
alpha-ketoaldehyde dehydrogenase
-
-
-
-
methylglyoxal dehydrogenase
-
-
-
-
NADP-dependent alpha-ketoaldehyde dehydrogenase
-
-
-
-
NADP-linked alpha-ketoaldehyde dehydrogenase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a 2-oxoaldehyde + NADP+ + H2O = a 2-oxo carboxylate + NADPH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
2-oxoaldehyde:NADP+ 2-oxidoreductase
Not identical with EC 1.2.1.23 2-oxoaldehyde dehydrogenase (NAD+).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
83588-97-0
-
97162-76-0
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconate + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconic acid + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconic acid + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
2-oxoaldehydes + NADP+ + H2O
2-oxo acids + NADPH
-
Substrates: -
Products: -
Products: -
?
2-oxoaldehydes + NADP+ + H2O
2-oxo acids + NADPH
-
Substrates: enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23
Products: -
Products: -
?
2-oxoaldehydes + NADP+ + H2O
2-oxo acids + NADPH
-
Substrates: -
Products: -
Products: -
?
2-oxoaldehydes + NADP+ + H2O
2-oxo acids + NADPH
-
Substrates: enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH
-
Substrates: contributes to the detoxification of methylglyoxal
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: ir
Products: -
Products: -
?
additional information
?
-
-
Substrates: the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: the presence of amines like Tris, 2-amino-2-methylpropane-1,3-diol, 2-amino-2-methylpropan-1-ol, 2-aminopropane-1,3-diol, 2-aminopropane or 2-aminobutan-1-ol is necessary to activate the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: L-serine methyl ester or L-2-aminopropan-1-ol are necessary to activate the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: the only known amine which activates the enzyme at physiological pH is L-serine methyl ester, L-2-amino-1-propanol gives very little activity but D,L-2-amino-1-propanol activates the enzyme
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
methylglyoxal + NADP+ + H2O
pyruvate + NADPH
-
Substrates: contributes to the detoxification of methylglyoxal
Products: -
Products: -
?
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconic acid + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconic acid + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
-
Substrates: ir
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
3-acetyl-pyridine adenine dinucleotide and thionicotinamide adenine dinucleotide are also effective oxidants
-
NAD+
-
Mgd1 and Mgd2 enzymes use also NAD+ as cofactor, see also EC 1.2.1.23
NAD+
-
enzyme from sheep or rat liver, see also EC 1.2.1.23
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.83
methylglyoxal
-
in the presence of L-2-aminopropan-1-ol (Km 83 mM), at pH 9.3 and 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200
-
using ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50 column, Blue-dextran affinity column and CM-Sephadex C-50 column
-
using ammonium sulfate fractionation and chromatography on DEAE-Sephadex column, Blue-dextran affinity column and CM-Sephadex column
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-200, hydroxylapatite, and DEAE-cellulose
-
using treatment with acetone, ammonium sulfate fractionation, and gel filtration on Sephadex G-25
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
detection of three Mgd loci, which show a restrictive tissue expression, Mgd1 and Mgd2 are preferentially expressed in liver and kidney, Mgd3 is expressed apparently exclusively in heart
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ray, S.; Ray, M.
Purification and characterization of NAD and NADP-linked alpha-ketoaldehyde dehydrogenases involved in catalyzing the oxidation of methylglyoxal to pyruvate
J. Biol. Chem.
257
10566-10570
1982
Capra hircus
brenda
Ray, M.; Ray, S.
On the interaction of nucleotides and glycolytic intermediates with NAD-linked alpha-ketoaldehyde dehydrogenase
J. Biol. Chem.
257
10571-10574
1982
Capra hircus
brenda
Dunkerton, J.; James, S.P.
Some observations on the NADP+-linked oxidation of methylglyoxal catalysed by 2-Oxoaldehyde dehydrogenase
Biochem. J.
153
503-504
1976
Ovis aries
brenda
Dunkerton, J.; James, S.P.
Purification of 2-oxoaldehyde dehydrogenase and its dependence on unusual amines
Biochem. J.
149
609-617
1975
Ovis aries
brenda
Jellum, E.
Metabolism of the ketoaldehyde 2-keto-3-deoxyglucose
Biochim. Biophys. Acta
165
357-363
1968
Ovis aries, Rattus norvegicus
brenda
Monder, C.
alpha-Keto aldehyde dehydrogenase, an enzyme that catalyzes the enzymic oxidation of methylglyoxal to pyruvate
J. Biol. Chem.
242
4603-4609
1967
Ovis aries
brenda
Bender, K.; Seibert, R.T.; Wienker, T.F.; Kren, V.; Pravenec, M.; Bissbort, S.
Biochemical genetics of methylglyoxal dehydrogenases in the laboratory rat (Rattus norvegicus)
Biochem. Genet.
32
147-154
1994
Rattus norvegicus
brenda
Vander Jagt, D.L.
2-Ketoaldehyde dehydrogenase from rat liver
Methods Enzymol.
89
513-515
1982
Rattus norvegicus
brenda
Vander Jagt, D.L.; Davidson, L.M.
Purification and characterization of 2-oxoaldehyde dehydrogenase from rat liver
Biochim. Biophys. Acta
484
260-267
1977
Rattus norvegicus
brenda
Vander Jagt, D.L.; Hunsaker, L.A.
Methylglyoxal metabolism and diabetic complications: roles of aldose reductase, glyoxalase-I, betaine aldehyde dehydrogenase and 2-oxoaldehyde dehydrogenase
Chem. Biol. Interact.
143-144
341-351
2003
Homo sapiens
brenda
EXTERNAL LINKS (specific for EC-Number 1.2.1.49)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
